What are the steps to prepare a slide to observe the structure of a plant cell?
Understand the Problem
The question outlines a procedure for observing the structure of a plant cell using an onion peel. It seeks to explain the steps needed to prepare the slide and what to look for under the microscope.
Answer
1. Peel onion membrane. 2. Place in distilled water. 3. Add safranine stain. 4. Transfer to slide. 5. Add glycerine. 6. Lower coverslip. 7. Remove excess. 8. Observe under microscope.
The steps to prepare a slide to observe the structure of a plant cell.
Answer for screen readers
The steps to prepare a slide to observe the structure of a plant cell.
More Information
Preparing slides for observation under a microscope helps highlight the structures within plant cells. Staining, typically using iodine or safranine, increases the contrast to make cell structures like the nucleus and cell wall more visible.
Tips
Common mistakes include failing to remove air bubbles when lowering the coverslip and not adequately staining the sample which can result in poor visibility of cell structures.
Sources
- Practical - preparing light microscope slides - Cell structure - Edexcel - bbc.co.uk
- Activity: Identifying Cells and Cell Parts Using a Microscope - manoa.hawaii.edu
- Plant Cell Slide Preparation - eiaab.com
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