The % base pair values of four nucleic acid samples are provided. Which sample has the highest Tm? (A) A = 31; T = 21; G = 20; C = 28 (B) A = 26; T = 14; G = 34; C = 26 (C) A = 17;... The % base pair values of four nucleic acid samples are provided. Which sample has the highest Tm? (A) A = 31; T = 21; G = 20; C = 28 (B) A = 26; T = 14; G = 34; C = 26 (C) A = 17; T = 19; G = 33; C = 31 (D) A = 20; T = 30; G = 25; C = 25
Understand the Problem
The question is asking us to determine which of the provided nucleic acid samples has the highest melting temperature (Tm) based on their given percentage base pair values.
Answer
Sample C has the highest Tm.
Sample C has the highest Tm.
Answer for screen readers
Sample C has the highest Tm.
More Information
The melting temperature (Tm) of nucleic acid samples is influenced by the GC-content, because guanine-cytosine pairs form three hydrogen bonds compared to the two formed by adenine-thymine pairs, making GC pairs more stable and resistant to denaturation. Sample C has the highest combined percentage of G and C at 64%.
Tips
A common mistake is not correctly calculating the GC content of the DNA, which is crucial as it determines stability and melting temperature of the DNA strand.
Sources
- GC-content - Wikipedia - en.wikipedia.org
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