Set 20 questions quiz for fourth year undergraduate students on the following: isolation of plasmid DNA, the significance of the reagents used in the technique.

A set of 20 quiz questions for fourth-year undergraduates on isolation of plasmid DNA and the significance of reagents used.

["1. What is the purpose of isolating plasmid DNA in molecular biology experiments?","2. What is the role of lysis buffer in the plasmid DNA isolation process?","3. Why is RNase A used during plasmid DNA purification?","4. Explain the significance of alkaline lysis in the isolation of plasmid DNA.","5. What step follows the use of alkaline lysis in plasmid DNA isolation?","6. How is plasmid DNA separated from chromosomal DNA during isolation?","7. What is the function of the neutralization buffer in plasmid DNA isolation?","8. Why is it important to use a cold ethanol solution in DNA precipitation?","9. What are some common methods for quantifying the concentration of isolated plasmid DNA?","10. What could be a reason for low yield in plasmid DNA isolation?","11. Describe one method to check the purity of isolated plasmid DNA.","12. Why might a researcher use a spin column during the plasmid DNA purification process?","13. What is the role of isopropanol in plasmid DNA isolation?","14. Which reagent is used to remove proteins during plasmid DNA purification?","15. Explain the use of silica matrices in plasmid DNA isolation.","16. What are the potential risks associated with the use of phenol during DNA purification?","17. What is the purpose of washing the DNA pellet with ethanol after centrifugation?","18. How does the quality of plasmid DNA affect downstream applications such as cloning?","19. What might cause contamination in a plasmid DNA prep and how can it be avoided?","20. Describe a troubleshooting step if no plasmid DNA is obtained after purification."]