Q3. Now, you finally get a better separation and understanding of the nature of the mixture. In brief, the mixture contains three classes of compounds. An analytical flow LC chroma... Q3. Now, you finally get a better separation and understanding of the nature of the mixture. In brief, the mixture contains three classes of compounds. An analytical flow LC chromatogram of these compounds is shown here (Figure 2) together with a zoomed view (upper panel of Figure 2). (4 marks total, 1 mark for each question under Q3). A. Describe what you can do to alter the gradient to better separate compounds highlighted in Figure 2 (Compound 1, 2, and 3). Draw your gradient with details. B. Suppose you can only load samples at half of the column's loading capacity. How many L.C. runs you need to meet sample amount requirements for LC-MS, NMR, and X-ray identification? C. Suppose for each minute, the cost for LC is around $S1. What is the total cost for collecting enough of these samples? D. Now, what type of LC and column you plan to use, and why?
Understand the Problem
The question is asking for a detailed response regarding the separation of compounds in a mixture using liquid chromatography techniques. It covers altering chromatographic gradients, calculating sample amounts needed for different analysis methods, and estimating costs for these analyses. Each part requires technical knowledge of chromatography and analytical chemistry.
Answer
Alter gradient for better separation; 1 run LC-MS, 5 NMR, 6 X-ray; Use reverse-phase LC with C18 column.
A. Alter the gradient to a slower, shallower gradient around the elution times of compounds. B. 1 run for LC-MS, 5 runs for NMR, 6 runs for X-ray. C. Total cost is the number of runs multiplied by the run time and cost per minute. D. Use reverse-phase LC with a C18 column.
Answer for screen readers
A. Alter the gradient to a slower, shallower gradient around the elution times of compounds. B. 1 run for LC-MS, 5 runs for NMR, 6 runs for X-ray. C. Total cost is the number of runs multiplied by the run time and cost per minute. D. Use reverse-phase LC with a C18 column.
More Information
Reverse-phase LC with C18 columns is commonly used for diverse compounds due to its ability to handle mixtures of varying polarity.
Tips
Ensure proper gradient alteration by observing compound elution times. Miscalculations in sample weights can lead to multiple unnecessary runs.
Sources
- Principles of chromatography | Stationary phase (article) - khanacademy.org
- Q. 26.23 A chromatogram of a two-componen... [FREE SOLUTION] - vaia.com
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