Draw cholinesterase activity vs pH plot.
Understand the Problem
The question involves calculations related to enzyme activity and requires drawing a plot of cholinesterase activity versus pH based on provided data. It combines concepts of biochemistry, specifically enzyme kinetics.
Answer
Plot cholinesterase activity against pH using the given data.
Use the given formula to calculate cholinesterase activity at different pH values, plot these on a graph with pH on the x-axis and enzyme activity on the y-axis.
Answer for screen readers
Use the given formula to calculate cholinesterase activity at different pH values, plot these on a graph with pH on the x-axis and enzyme activity on the y-axis.
More Information
Cholinesterase activity is influenced by pH, typically increasing up to a certain point before declining as pH becomes too high or too low.
Tips
Ensure precise measurements of absorbance changes at 412 nm and consistent sample preparation to accurately compute enzyme activity.
Sources
- The Relationship between pH and the Activity of Cholinesterase - jstor.org
- Effect of pH on inhibition and spontaneous reactivation - pmc.ncbi.nlm.nih.gov
- Effect of pH on the activity of nervous cholinesterases - link.springer.com
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