1. Determine the gene and protein sequence for the lactate dehydrogenase (LDH) gene from Bacillus subtilis str 168. Report these in FASTA form. 2. From what you learn on the NCBI G... 1. Determine the gene and protein sequence for the lactate dehydrogenase (LDH) gene from Bacillus subtilis str 168. Report these in FASTA form. 2. From what you learn on the NCBI GenBank View_NP page, please comment on each of the following: a. What redox cofactor does LDH use? (NAD, FAD, NADP, or Heme). b. This enzyme converts (S)-lactate and NAD+ to what two products? c. How many amino acids are present in LDH from B. subtilis?
Understand the Problem
The question is asking for information about the lactate dehydrogenase (LDH) gene from Bacillus subtilis, including its gene and protein sequences, redox cofactor usage, conversion products, and the number of amino acids in the enzyme.
Answer
1. Get sequences in FASTA form. 2a. NAD. 2b. Pyruvate, NADH. 2c. 316 amino acids.
The final answer is: 1. Use NCBI GenBank and UniProt databases to find and format LDH sequences in FASTA form. 2a. The redox cofactor used is NAD. 2b. The enzyme converts (S)-lactate and NAD+ to pyruvate and NADH. 2c. LDH from B. subtilis has 316 amino acids.
Answer for screen readers
The final answer is: 1. Use NCBI GenBank and UniProt databases to find and format LDH sequences in FASTA form. 2a. The redox cofactor used is NAD. 2b. The enzyme converts (S)-lactate and NAD+ to pyruvate and NADH. 2c. LDH from B. subtilis has 316 amino acids.
More Information
Lactate dehydrogenase in Bacillus subtilis is responsible for catalyzing the conversion of substrates in glycolytic and gluconeogenic pathways, utilizing NAD as a cofactor.
Tips
Ensure the correct strain and organism is selected when searching for sequences. Cross-reference multiple databases for accuracy.
Sources
- ldh - L-lactate dehydrogenase - Bacillus subtilis (strain 168) - UniProt - uniprot.org
- GenBank Overview - NCBI - ncbi.nlm.nih.gov
- 3PQD: Crystal structure of L-lactate dehydrogenase from Bacillus ... - rcsb.org
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