30 Questions
What type of regulation is exhibited by the Lac Operon?
Inducible regulation
Which protein allows for positive gene regulation in the Lac Operon?
Activator protein
What is the main role of the cAMP-dependent catabolite activator protein (CAP) in the Lac Operon?
Activation of transcription in the absence of glucose
Which type of gene regulation is associated with the LacI repressor protein?
Negative gene regulation
What synchronizes sets of operons controlled by a single regulator protein?
Regulon
What is the consequence of Repressor protein binding in the Lac Operon?
Reduction of RNA polymerase binding
Which proteins alter gene expression by acting on DNA binding sites?
Activator & Repressor proteins
What are sets of operons controlled by a single regulator protein called?
Regulons
What is the main function of the LacI repressor protein in the Lac Operon?
Inhibiting gene expression
Which molecule binds to the cAMP-dependent catabolite activator protein (CAP) in the absence of glucose in the Lac Operon?
cAMP
What is the consequence of an inducer binding to the LacI repressor protein in the Lac Operon?
Prevents the repressor from binding to the operator
Which type of mRNA is produced in prokaryotes that allows for the co-transcription of multiple genes?
Polycistronic mRNA
What is the role of activator proteins in prokaryotic gene regulation?
Promoting RNA polymerase binding
In the absence of glucose, what is the status of cAMP levels and CAP activity in the Lac Operon?
High cAMP levels, high CAP activity
What is the function of a repressor protein in prokaryotic gene regulation?
Inhibit transcription initiation
What is unique about monocistronic mRNA compared to polycistronic mRNA?
It codes for a single protein only
What is the effect of lactose availability on Lac Operon gene expression?
Induces CAP-cAMP complex formation
Which regulator protein binds directly to DNA in prokaryotic gene regulation?
Repressor protein
What is the role of DNA binding proteins in gene regulation?
Suppress transcription
In prokaryotes, what type of mRNA allows for the co-transcription of multiple genes?
Polycistronic mRNA
Which DNA segment do regulatory proteins bind to in gene regulation?
Promoter site
In prokaryotes, what is the function of monocistronic mRNA?
Express a single gene per transcript
What characterizes genes usually found in an operon?
'One promoter to one gene' organization
What type of mRNA is produced in eukaryotes?
Monocistronic mRNA
What is the function of DNA binding proteins in gene regulation?
DNA binding proteins bind to specific regulatory DNA sequences to enhance or suppress transcription.
What is the characteristic of genes usually found in an operon?
Bacterial genes usually found in an operon are functionally related and efficiently packed on the chromosome.
How do DNA binding proteins enhance or suppress transcription?
DNA binding proteins bind to operator sites adjacent to or overlapping promoter sites to regulate transcription.
What type of mRNA is produced in prokaryotes that allows for the co-transcription of multiple genes?
Prokaryotes produce polycistronic mRNA that allows for the co-transcription of multiple genes.
What is the role of operons in bacterial genes?
Operons in bacterial genes help in the coordinated regulation and expression of functionally related genes.
Which proteins alter gene expression by acting on DNA binding sites?
DNA binding proteins (BPs) alter gene expression by binding to specific regulatory DNA sequences.
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