Diagnosis of Infectious Disease
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Questions and Answers

What is the primary goal of genomics in disease control?

  • To prove immunity due to past infection or vaccination
  • To cultivate pathogens directly
  • To characterize pathogens and help control the disease they cause (correct)
  • To reduce the analytical error in immune assays

What is the primary advantage of laboratory automation?

  • It increases the analytical error in immune assays
  • It helps in cultivating pathogens directly
  • It is essential for indirect diagnosis
  • It reduces the time and increases the throughput of samples (correct)

What is the first antibody to appear in most patients after exposure?

  • IgE
  • IgM (correct)
  • IgA
  • IgG

What is the primary application of WGA in SARSCov-2 surveillance?

<p>Surveillance of VOI and VOC (D)</p> Signup and view all the answers

What is the primary purpose of serodiagnosis?

<p>To detect the presence of antibodies in a patient's serum (C)</p> Signup and view all the answers

What is syndromic testing used for?

<p>To diagnose a range of infectious diseases (D)</p> Signup and view all the answers

What is multiplex PCR used for?

<p>To amplify several DNA sequences simultaneously (C)</p> Signup and view all the answers

What is the benefit of using ELISA in immune assays?

<p>It is a highly sensitive and specific test (A)</p> Signup and view all the answers

What is the primary limitation of syndromic testing?

<p>It is expensive (B)</p> Signup and view all the answers

What is the primary advantage of indirect diagnosis?

<p>It is used to detect the presence of antibodies in a patient's serum (C)</p> Signup and view all the answers

What is the primary reason for diagnosing an infection?

<p>To determine the infectious agent (A)</p> Signup and view all the answers

What is the purpose of infection control in relation to diagnosis?

<p>To prevent the spread of infection (D)</p> Signup and view all the answers

What type of sample can be collected for laboratory tests?

<p>Various types including blood, saliva, urine, pus, and feces (A)</p> Signup and view all the answers

Why is it essential to minimize specimen contamination during collection?

<p>To obtain accurate test results (A)</p> Signup and view all the answers

What is the importance of collecting sufficient quantity of specimens?

<p>To allow for complete microbiologic examination (C)</p> Signup and view all the answers

Why is it essential to determine the type of infection?

<p>To determine the appropriate treatment and management (A)</p> Signup and view all the answers

What is the primary purpose of Matrix-assisted laser desorption/ionization time of flight (MALDI-TOF)?

<p>To identify the species of microorganisms through protein peak analysis (D)</p> Signup and view all the answers

What is the process by which protein molecules are converted into ions in the gas phase in MALDI-TOF?

<p>A laser strikes the sample (A)</p> Signup and view all the answers

What is the principle behind the measurement of time of flight in MALDI-TOF?

<p>Lighter ions take less time to travel to the detector (D)</p> Signup and view all the answers

What is the primary role of the matrix solution in MALDI-TOF?

<p>To allow the sample to dry (B)</p> Signup and view all the answers

What is the primary advantage of using MALDI-TOF for microbial identification?

<p>It is a rapid and accurate method for identifying microorganisms (C)</p> Signup and view all the answers

What is the primary limitation of colony and cellular morphology in microbial identification?

<p>It may not provide a definitive identification (C)</p> Signup and view all the answers

What is the primary advantage of using serology in diagnostic methods?

<p>It can provide indirect evidence of past infection or vaccination (A)</p> Signup and view all the answers

What is the primary application of genomics in disease control?

<p>To characterize pathogens and help control the disease they cause (C)</p> Signup and view all the answers

What is the primary advantage of multiplex PCR?

<p>It can detect multiple DNA sequences simultaneously (B)</p> Signup and view all the answers

What is the primary goal of syndromic testing?

<p>To provide a rapid diagnosis of common infectious diseases (D)</p> Signup and view all the answers

What is the primary limitation of syndromic testing?

<p>It is a costly approach (A)</p> Signup and view all the answers

What is the primary role of IgM in immune assays?

<p>To appear first in most patients after exposure (B)</p> Signup and view all the answers

What is the most suitable diagnostic test for a 26-year-old teacher who has been in contact with a measles case at school?

<p>Both Measles IgM and IgG (C)</p> Signup and view all the answers

What is the significance of a negative Measles IgM and a positive Measles IgG result in the teacher?

<p>The patient is immune to measles (B)</p> Signup and view all the answers

What type of sample is required to diagnose a sore throat caused by Streptococcus pyogenes?

<p>Charcoal swab (B)</p> Signup and view all the answers

What is the characteristic of Streptococcus pyogenes growth on blood agar?

<p>Beta haemolysis (D)</p> Signup and view all the answers

What is the appropriate antibiotic choice for treating pharyngitis caused by Streptococcus pyogenes?

<p>Penicillin or amoxicillin (A)</p> Signup and view all the answers

What is the significance of a positive Lancefield group A result in a throat swab?

<p>It indicates the presence of Streptococcus pyogenes (D)</p> Signup and view all the answers

What is essential when collecting specimens for microbiologic examination?

<p>Obtaining enough tissue for both histopathologic and microbiologic examination (D)</p> Signup and view all the answers

What is the purpose of selective media in microbiologic diagnosis?

<p>To permit the isolation of specific types of microorganisms (D)</p> Signup and view all the answers

Which of the following is a direct diagnosis method?

<p>Immunofluorescence (D)</p> Signup and view all the answers

What is a key consideration when diagnosing infections in the lab?

<p>How fast do we need the result? (B)</p> Signup and view all the answers

What is an additional step in microbial identification?

<p>Colony and cellular morphology (D)</p> Signup and view all the answers

What is the purpose of antimicrobial susceptibility testing?

<p>To determine the best treatment option (C)</p> Signup and view all the answers

The GI tract is essentially a long ______, ascending from the mouth to the anus.

<p>tube</p> Signup and view all the answers

The GI tract is heavily colonised with ______ and viruses and encounters millions of new ones every day.

<p>microorganisms</p> Signup and view all the answers

All intestinal surfaces are coated with layers of ______, which provides mechanical protection.

<p>mucus</p> Signup and view all the answers

The muscular surfaces of the GI tract ensures movement termed ______.

<p>peristalsis</p> Signup and view all the answers

Various fluids in the GI tract also have ______ properties.

<p>antimicrobial</p> Signup and view all the answers

Normal ______ is also vitally important to prevent infection.

<p>flora</p> Signup and view all the answers

The respiratory system is the most commonly infected ______ system.

<p>human</p> Signup and view all the answers

Health care providers will see more respiratory infections than any other type of ______.

<p>infection</p> Signup and view all the answers

The respiratory tract is divided into two ______ tracts: upper and lower.

<p>respiratory</p> Signup and view all the answers

The upper respiratory tract includes the ______, sinuses, pharynx, and larynx.

<p>nasal</p> Signup and view all the answers

Respiratory pathogens can be transmitted from human to human and can ______ within a community.

<p>circulate</p> Signup and view all the answers

Some respiratory pathogens exist as part of the normal ______ flora.

<p>human</p> Signup and view all the answers

The respiratory viruses that most commonly circulate in all continents as endemic or epidemic agents are ______ virus and others.

<p>influenza</p> Signup and view all the answers

Respiratory Syncytial Virus is an ______ RNA virus and belongs to the Paramyxoviridae family.

<p>enveloped</p> Signup and view all the answers

The very young (under ______ year of age) and the elderly are at the greatest risk of developing severe disease.

<p>1</p> Signup and view all the answers

Diagnosis is typically made via ______-transcription real-time PCR (RNA) or RT-PCR (DNA).

<p>reverse</p> Signup and view all the answers

Children born prematurely, or with underlying ______ lung disease, are also at increased risk of developing severe disease.

<p>chronic</p> Signup and view all the answers

Monitored by UKHSA, outbreaks of seasonal ______/Influenza occur every year.

<p>RSV</p> Signup and view all the answers

Lower respiratory tract infection can be caused by a virus or so-called ‘atypical’ bacteria, including ______ pneumoniae.

<p>Mycoplasma</p> Signup and view all the answers

Nosocomial pneumonia usually occurs approximately ______ hours after admission to hospital.

<p>48</p> Signup and view all the answers

Aspiration pneumonia is a major problem in ______ patients.

<p>hospital</p> Signup and view all the answers

Community-acquired pneumonia usually presents as a ______ pneumonia.

<p>lobar</p> Signup and view all the answers

Staphylococcus aureus is usually associated with ______ pneumonia.

<p>Nosocomial</p> Signup and view all the answers

Fluid losses of nearly 1 litre per hour have been reported in severe cases of a disease, and an untreated patient can lose up to 50% of ______ during the course of the disease.

<p>body weight</p> Signup and view all the answers

Don’t forget about ______-2 in lower respiratory tract infections.

<p>SARS-CoV</p> Signup and view all the answers

The voided fluid contains flecks of ______, giving the description of ‘rice-water stool’.

<p>mucus</p> Signup and view all the answers

______ Spp Thioshulphate citrate bile salts (TCBS) Agar is used for the selective isolation of cholera vibrios and Vibrio parahaemolyticus from a variety of clinical and nonclinical specimens.

<p>Vibrio</p> Signup and view all the answers

Clostridium difficile is a Gram-positive, ______ rod-shaped bacterium found as part of the normal intestinal biota.

<p>endosporeforming</p> Signup and view all the answers

The predominant symptoms of C. difficile is ______, with abdominal cramps, fever and leucocytosis in more severe cases.

<p>diarrhoea</p> Signup and view all the answers

Gastritis is experienced as a sharp or burning ______ emanating from the upper abdomen.

<p>pain</p> Signup and view all the answers

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