23 Questions
Which type of proteins bind DNA exclusively as homo- or heterodimers with their extended alpha-helices?
Leucine zipper proteins
What is the characteristic feature of helix-loop-helix (HLH) proteins?
They are characterized by two alpha-helices connected by a short loop
What is the main function of the GAL4 transcription factor from yeast?
To stimulate transcription
Which type of zinc finger DNA binding domains usually contain three or more finger units and bind to DNA as monomers?
C2H2 types
What is the role of the Mediator complex in transcription?
To bridge vast sections of chromatin to enhance transcriptional initiation
What is the function of homeodomain proteins?
To bind homeodomain binding sites
What is the characteristic feature of C6 Zinc Finger transcription factor?
It contains six cysteine metal ligands that coordinately bind two Zn2+ ions
What is the purpose of ChIP-Chromatin Immunoprecipitation (ChIP-seq)?
To determine DNA binding elements
What is the main function of leucine zipper proteins?
To bind DNA exclusively as homo- or heterodimers with their extended alpha-helices
What do helix-loop-helix (HLH) proteins contain in the C-terminal region of the DNA binding domain?
Hydrophobic amino acids spaced at intervals characteristic of an amphipathic alpha-helix
What is the role of transcription factors of unrelated classes in binding DNA?
They can bind cooperatively to enhance transcriptional responses
What is used as a probe in Electrophoretic mobility shift assays (EMSA)?
Radiolabelled dsDNA segment
What type of genes are regulated by multiple transcriptional control elements in eukaryotes?
Most genes
Which of the following is a common reporter gene used for relative quantification of transcriptional efficacy?
GFP
What type of assay is used to test DNA binding transcription factors?
Electrophoretic mobility shift assay (EMSA)
What is altered in the non-denaturing polyacrylamide gel in EMSA?
Protein:DNA mobility
What do transcription factors recognize in DNA sequences?
Specific DNA sequence motifs
What domain contains the recognition helix in transcription factors?
Alpha-helical domain
What type of interactions occur during the binding of transcription factors to DNA?
Noncovalent interactions
What is co-transfected with the candidate protein in the assay to test DNA binding transcription factors?
Plasmid 1 and 2
What is not revealed by EMSA/Gel Shifts?
The precise sequence bound by the protein
What is used for DNA binding activity in EMSA?
Gel/band/mobility shift assays
Which of the following is a subregion of the promoter required for activation of transcription?
Thymidine kinase (tk)
Test your knowledge of transcriptional regulation in eukaryotic genes with this McGill BIOL200 quiz. Topics include proteins that regulate transcription, activators, cis-acting regulatory sites, and reporter genes for quantifying transcriptional efficacy. Prepare for your exam by practicing with this quiz.
Make Your Own Quizzes and Flashcards
Convert your notes into interactive study material.
Get started for free
