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TLC and HPTLC in Analytical Chemistry

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Questions and Answers

What is the purpose of the TLC-MS Interface in HPTLC?

To visualize separated compounds on the plate
To calculate the Rf values of separated compounds
To directly identify compounds that respresnt each spot through it's molecular weight (correct)
To detect substances through fluorescence mode

What is measured in the TLC Scanner with Video Scan Detection?

The identity of substances through derivatization reactions
The Rf values of separated compounds
The mass spectra of separated compounds
The reflection of separated compounds in absorbance and/or fluorescence mode (correct)

What is the purpose of derivatization reactions in TLC?

To visualize separated compounds on the plate (correct)
To identify the structure of separated compounds
To separate compounds based on their molecular weight
To calculate the Rf values of separated compounds

What is the unit of measurement for the Rf value?

Unitless ratio (A) Signup and view all the answers

What is the advantage of using the TLC-MS Interface?

It enables the direct analysis of zones from TLC and HPTLC plates by Mass Spectrometry (D) Signup and view all the answers

What is the result of analyzing zones from TLC and HPTLC plates by Mass Spectrometry?

A substance confirmation in less than a minute via its mass spectrum (A) Signup and view all the answers

What is a crucial consideration when comparing Rf values?

The conditions under which the TLC plates were run (B) Signup and view all the answers

Which of the following groups uses TLC to monitor chemical reactions in real-time?

Researchers (A) Signup and view all the answers

What can be concluded if two substances have different Rf values?

They are definitely different substances (A) Signup and view all the answers

What is an application of TLC in drug analysis?

Determining the purity of manufactured drugs (A) Signup and view all the answers

What is the purpose of a reference compound in TLC?

To verify the identity of an unknown compound (C) Signup and view all the answers

What is the range of Rf values?

Zero to one (C) Signup and view all the answers

What is the primary advantage of using High Performance Thin Layer Chromatography (HPTLC) over conventional Thin Layer Chromatography (TLC)?

Improved speed and optimized mobile phase flow (C) Signup and view all the answers

Which of the following reagents is used to detect zones on a TLC plate with a yellow color?

N,N dimethyl amino benzene (D) Signup and view all the answers

What is the primary function of the TLC Visualizer in HPTLC?

Detection of even the faintest zones (A) Signup and view all the answers

What is the importance of automatic sampling in HPTLC?

It ensures the precision of the applied volume and exact positioning (A) Signup and view all the answers

What is the primary advantage of using standardized methodology in HPTLC?

It ensures reproducible results (C) Signup and view all the answers

What is the purpose of using suitable software in HPTLC?

To create a virtual plate for comparison (A) Signup and view all the answers

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