18 Questions
What is the purpose of the TLC-MS Interface in HPTLC?
To directly identify compounds that respresnt each spot through it's molecular weight
What is measured in the TLC Scanner with Video Scan Detection?
The reflection of separated compounds in absorbance and/or fluorescence mode
What is the purpose of derivatization reactions in TLC?
To visualize separated compounds on the plate
What is the unit of measurement for the Rf value?
Unitless ratio
What is the advantage of using the TLC-MS Interface?
It enables the direct analysis of zones from TLC and HPTLC plates by Mass Spectrometry
What is the result of analyzing zones from TLC and HPTLC plates by Mass Spectrometry?
A substance confirmation in less than a minute via its mass spectrum
What is a crucial consideration when comparing Rf values?
The conditions under which the TLC plates were run
Which of the following groups uses TLC to monitor chemical reactions in real-time?
Researchers
What can be concluded if two substances have different Rf values?
They are definitely different substances
What is an application of TLC in drug analysis?
Determining the purity of manufactured drugs
What is the purpose of a reference compound in TLC?
To verify the identity of an unknown compound
What is the range of Rf values?
Zero to one
What is the primary advantage of using High Performance Thin Layer Chromatography (HPTLC) over conventional Thin Layer Chromatography (TLC)?
Improved speed and optimized mobile phase flow
Which of the following reagents is used to detect zones on a TLC plate with a yellow color?
N,N dimethyl amino benzene
What is the primary function of the TLC Visualizer in HPTLC?
Detection of even the faintest zones
What is the importance of automatic sampling in HPTLC?
It ensures the precision of the applied volume and exact positioning
What is the primary advantage of using standardized methodology in HPTLC?
It ensures reproducible results
What is the purpose of using suitable software in HPTLC?
To create a virtual plate for comparison
This quiz covers the principles and applications of Thin Layer Chromatography (TLC) and High Performance Thin Layer Chromatography (HPTLC) in analytical chemistry, including instrumentations and detection methods.
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