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24 Chromatography & Instrumentation MCQ1

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15 Questions

What is the principle behind the separation of positively charged ions in a mass spectrometer?

Their relative atomic masses

What is the purpose of vaporisation in a mass spectrometer?

To break the molecules into ions and free electrons

What is the formula for Rf in chromatography?

Distance travelled by the component / Distance travelled by the solvent front

What type of spectrum is obtained in infrared spectroscopy?

Absorption spectrum

What is the relationship between absorbance and concentration in ultraviolet absorption spectroscopy?

Absorbance is directly proportional to concentration

Which type of chromatography uses a solid stationary phase and a liquid mobile phase?

Column chromatography

What is the principle of High Performance Liquid Chromatography?

Different components of a mixture have different tendencies to adsorb onto very fine particles of a solid

What is the purpose of passing UV light through a sample and a blank in ultraviolet absorption spectroscopy?

To obtain an absorption spectrum

What is the purpose of the flame in Atomic Absorption Spectroscopy?

To convert the sample element into atoms of that element

What is the result of the absorption of infrared radiation by molecules in infrared spectroscopy?

The molecules are excited to a higher energy state

What is the relationship between absorbance and concentration in Atomic Absorption Spectroscopy?

Absorbance is directly proportional to concentration

What type of pollutants can be identified using infrared spectroscopy?

Organic pollutants in water

What is the main application of Chromatography in food analysis?

All of the above

What is the purpose of obtaining an absorption spectrum in ultraviolet absorption spectroscopy?

To identify the presence of a specific substance

What is the main difference between Gas Chromatography and High Performance Liquid Chromatography?

The type of mobile phase used

Test your understanding of chromatography, its principles, processes, and uses in organic chemistry. Learn about different types of chromatography, such as paper, thin layer, and column chromatography. Perfect for chemistry students and professionals.

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