24 Chromatography and Instrumentation SAQ1
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Questions and Answers

What is the principle on which positively charged ions are separated in a Mass Spectrometer?

Their relative atomic masses

What is the purpose of vaporisation in a Mass Spectrometer?

To convert a substance into its gaseous state

How does the absorbance of ultraviolet radiation relate to the concentration of a substance?

Absorbance is directly proportional to concentration

What type of radiation is used to detect the absorption of molecules in Infrared Spectroscopy?

<p>Infrared radiation</p> Signup and view all the answers

What is the fundamental principle behind chromatography?

<p>Different components of a mixture have different interactions with the stationary and mobile phases.</p> Signup and view all the answers

What is the formula for Rf in chromatography?

<p>Rf = Distance travelled by the component / Distance travelled by the solvent front</p> Signup and view all the answers

What is the purpose of the stationary phase in chromatography?

<p>To interact with the components of the mixture, causing them to separate</p> Signup and view all the answers

What is the difference between High Performance Liquid Chromatography (HPLC) and Gas Chromatography?

<p>HPLC uses a liquid mobile phase, while Gas Chromatography uses a gas mobile phase.</p> Signup and view all the answers

What are some examples of applications of chromatography in food analysis?

<p>Analysis of growth promoters in food and analysis of vitamins in food</p> Signup and view all the answers

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