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24 Chromatography and Instrumentation SAQ1

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What is the principle on which positively charged ions are separated in a Mass Spectrometer?

Their relative atomic masses

What is the purpose of vaporisation in a Mass Spectrometer?

To convert a substance into its gaseous state

How does the absorbance of ultraviolet radiation relate to the concentration of a substance?

Absorbance is directly proportional to concentration

What type of radiation is used to detect the absorption of molecules in Infrared Spectroscopy?

Infrared radiation

What is the fundamental principle behind chromatography?

Different components of a mixture have different interactions with the stationary and mobile phases.

What is the formula for Rf in chromatography?

Rf = Distance travelled by the component / Distance travelled by the solvent front

What is the purpose of the stationary phase in chromatography?

To interact with the components of the mixture, causing them to separate

What is the difference between High Performance Liquid Chromatography (HPLC) and Gas Chromatography?

HPLC uses a liquid mobile phase, while Gas Chromatography uses a gas mobile phase.

What are some examples of applications of chromatography in food analysis?

Analysis of growth promoters in food and analysis of vitamins in food

Test your understanding of chromatography, its principles, processes, and uses in organic chemistry. Learn about different types of chromatography, including paper, thin layer, and column chromatography.

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