18 Questions
What is the primary purpose of the ion source in a GC-MS instrument?
To ionize and fragment the remaining molecules
Which of the following applications is NOT typically associated with GC-MS analysis?
Astronomy
What is the relationship between the area under a peak in a chromatogram and the amount of analyte injected?
The area under the peak is directly proportional to the amount of analyte
What is the primary function of the mass analyzer in a GC-MS instrument?
To separate the ions by their mass-to-charge ratio
Which of the following is a quantitative application of GC-MS?
Analysis of a diverse array of samples
What is typically used to determine the peak area in a chromatogram?
All of the above
Which detector type is selective for compounds containing phosphorus and sulfur?
Flame photometric detector
What is the primary advantage of using GC-MS with continuous total ion scan?
Ability to identify a mixture's components using mass spectra
Which of the following detectors is not typically used for analyte identification?
Flame ionization detector (FID)
What is the primary purpose of selective-ion monitoring in GC-MS?
To achieve some degree of selectivity for specific analytes
What is the typical range of the limit of detection (LOD) for GC-MS?
25 fg to 100 pg
Which of the following is a benefit of using GC-MS with continuous recording of the mass spectrum?
Ability to examine the mass spectrum for any time increment
What is the primary advantage of using fluorescence detection in HPLC?
It provides higher sensitivity and selectivity compared to UV detection
In reversed-phase HPLC, what is the primary factor that determines the retention of organic molecules in the column?
Their degree of hydrophobicity
In ion-exchange chromatography, what is the primary factor that determines the elution order of organic acids and bases?
Their pKa or pKb values
What is the primary mechanism of separation in gel filtration chromatography?
Size exclusion of analytes based on their molecular size and shape
What is the primary advantage of using mass spectrometry in detection?
It allows for the specific identification of compounds based on their mass-to-charge ratio
What is the primary purpose of using a flow cell in HPLC detection?
To allow for the measurement of differential light output
Understand the principles of Gas Chromatography-Mass Spectrometry (GC-MS) instrumentation, including the ionization chamber, mass analyzer, and detector. Learn about the applications of GC and GC-MS in various fields.
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