15 Questions
What is the purpose of using tissue processing baskets?
To transfer tissues from reagent to reagent
What is the most satisfactory embedding medium used in routine histology?
Paraffin wax
What is the principle of tissue processing?
Replacing tissue water with paraffin wax
Why is it necessary to remove tissue water before embedding the tissue in paraffin wax?
To prevent damage to the tissue during sectioning
Which process occurs before tissue impregnation with paraffin wax?
Fixation
What is the purpose of using a tissue tek system?
To write the tissue identity on the cassette
What factor influences the rate of tissue impregnation by paraffin wax?
Agitation
Which method can be used to achieve efficient agitation for tissue impregnation?
Pumping system
What does heat do to the rate of tissue penetration by paraffin wax?
Increases the rate
What influences the viscosity and rate of penetration of tissue impregnation?
Molecule size
What is the first step in paraffin wax embedding?
Fixation
Why is it inadvisable to use a higher concentration of alcohol initially for dehydration?
It causes rapid removal of water, leading to cell shrinkage
What is the purpose of using anhydrous copper sulphate in higher grade dehydrating alcohols?
To remove water from alcohol
What does the change of colour of copper sulphate from white to blue indicate?
Complete tissue dehydration
In which type of fixative is tissue transferred directly to 95% alcohol without prior dehydration?
Heidenhain's Susa fixative
Test your knowledge of tissue processing baskets and the Tissue Tek system with this quiz. Learn about the principles of tissue processing and the use of these systems to ensure correct labeling and retention of tissue identity during sectioning and storage.
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