Tissue Processing Baskets and Tissue Tek System Quiz
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Questions and Answers

What is the purpose of using tissue processing baskets?

  • To make the tissue more rigid
  • To store tissue blocks permanently
  • To remove tissue water and replace it with a solid medium
  • To transfer tissues from reagent to reagent (correct)

What is the most satisfactory embedding medium used in routine histology?

  • Tissue processing baskets
  • Lipid tissue fluids
  • Paraffin wax (correct)
  • Aqueous fixatives

What is the principle of tissue processing?

  • Thoroughly permeating the tissue with fluid medium
  • Storing tissue blocks in tissue tek system
  • Using aqueous fixatives to remove lipid tissue fluids
  • Replacing tissue water with paraffin wax (correct)

Why is it necessary to remove tissue water before embedding the tissue in paraffin wax?

<p>To prevent damage to the tissue during sectioning (C)</p> Signup and view all the answers

Which process occurs before tissue impregnation with paraffin wax?

<p>Fixation (C)</p> Signup and view all the answers

What is the purpose of using a tissue tek system?

<p>To write the tissue identity on the cassette (B)</p> Signup and view all the answers

What factor influences the rate of tissue impregnation by paraffin wax?

<p>Agitation (B)</p> Signup and view all the answers

Which method can be used to achieve efficient agitation for tissue impregnation?

<p>Pumping system (D)</p> Signup and view all the answers

What does heat do to the rate of tissue penetration by paraffin wax?

<p>Increases the rate (D)</p> Signup and view all the answers

What influences the viscosity and rate of penetration of tissue impregnation?

<p>Molecule size (A)</p> Signup and view all the answers

What is the first step in paraffin wax embedding?

<p>Fixation (B)</p> Signup and view all the answers

Why is it inadvisable to use a higher concentration of alcohol initially for dehydration?

<p>It causes rapid removal of water, leading to cell shrinkage (B)</p> Signup and view all the answers

What is the purpose of using anhydrous copper sulphate in higher grade dehydrating alcohols?

<p>To remove water from alcohol (D)</p> Signup and view all the answers

What does the change of colour of copper sulphate from white to blue indicate?

<p>Complete tissue dehydration (A)</p> Signup and view all the answers

In which type of fixative is tissue transferred directly to 95% alcohol without prior dehydration?

<p>Heidenhain's Susa fixative (A)</p> Signup and view all the answers

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