Podcast
Questions and Answers
Acetone at 0-4°C is widely used for fixation of tissues intended to study which of the following?
Acetone at 0-4°C is widely used for fixation of tissues intended to study which of the following?
Acrolein and chromyl chloride are used at 37°C for 1-2 hours for fixation of tissues intended to study which of the following?
Acrolein and chromyl chloride are used at 37°C for 1-2 hours for fixation of tissues intended to study which of the following?
What is absolute alcohol commonly used for in the context of tissue fixation?
What is absolute alcohol commonly used for in the context of tissue fixation?
Which technique involves using a mordant for tissues?
Which technique involves using a mordant for tissues?
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When is it sometimes useful to submit the tissue to a second fixative after fixation in formalin?
When is it sometimes useful to submit the tissue to a second fixative after fixation in formalin?
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Study Notes
Fixatives and Fixation Methods
- Formaldehyde vapour is obtained by heating paraformaldehyde.
- Paraformaldehyde vapour is obtained by heating paraformaldehyde between 50° and 80°C.
- Blocks of tissue require 3-5 hours for fixation, whereas sections require 1⁄2-1 hour.
- Acetaldehyde vapour is obtained by heating at 80°C for 1-4 hours.
- Glutaraldehyde is used as a 50% aqueous solution at 80°C for 2 min to 4 hours.
- Acrolein/chromyl chloride is used at 37°C for 1-2 hours.
- Fixatives include formal saline and cold acetone, which is widely used for fixation of tissues intended to study enzymes, especially when immersing in acetone at a low temperature.
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Description
Test your knowledge of tissue processing with this quiz. From obtaining formaldehyde vapor to processing blocks of tissue, this quiz covers key concepts and techniques.
