5 Questions
Acetone at 0-4°C is widely used for fixation of tissues intended to study which of the following?
Enzymes
Acrolein and chromyl chloride are used at 37°C for 1-2 hours for fixation of tissues intended to study which of the following?
Enzymes
What is absolute alcohol commonly used for in the context of tissue fixation?
Fixation for 24 hours
Which technique involves using a mordant for tissues?
Fixation in formalin
When is it sometimes useful to submit the tissue to a second fixative after fixation in formalin?
After processing when making sections
Study Notes
Fixatives and Fixation Methods
- Formaldehyde vapour is obtained by heating paraformaldehyde.
- Paraformaldehyde vapour is obtained by heating paraformaldehyde between 50° and 80°C.
- Blocks of tissue require 3-5 hours for fixation, whereas sections require 1⁄2-1 hour.
- Acetaldehyde vapour is obtained by heating at 80°C for 1-4 hours.
- Glutaraldehyde is used as a 50% aqueous solution at 80°C for 2 min to 4 hours.
- Acrolein/chromyl chloride is used at 37°C for 1-2 hours.
- Fixatives include formal saline and cold acetone, which is widely used for fixation of tissues intended to study enzymes, especially when immersing in acetone at a low temperature.
Test your knowledge of tissue processing with this quiz. From obtaining formaldehyde vapor to processing blocks of tissue, this quiz covers key concepts and techniques.
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