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Tissue Embedding and Dehydration Process

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Tissue Embedding and Dehydration Process

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Questions and Answers

What is the typical thickness of paraffin sections for light microscopy?

Less than 1 μm

3-10 μm (correct)

1 μm

10 nm

Which spatial unit is commonly used in microscopy that is equivalent to 10−4 μm?

Millimeter (mm)

Nanometer (nm)

Angstrom (Å) (correct)

Centimeter (cm)

How are frozen sections useful in histochemical studies compared to fixed tissues?

They preserve enzyme activity

They provide clearer microscopic images

They do not dissolve cell lipids (correct)

They preserve cell structures better

Which dye behaves like a basic dye and stains basophilic tissue components?

Hematoxylin Signup and view all the answers

What is the purpose of staining tissue sections in microscopy?

To make components distinguishable Signup and view all the answers

What is the purpose of dehydrating fixed tissues before embedding?

To remove water from the tissue gradually Signup and view all the answers

Why is embedding with plastic resin preferred over paraffin for some tissues?

Plastic resin embedding avoids tissue distortion due to lower required temperatures. Signup and view all the answers

What is the purpose of the clearing step in tissue preparation?

To give the tissue a translucent appearance Signup and view all the answers

Why is it important to trim the hardened block before sectioning?

To avoid damage to the microtome blade during sectioning Signup and view all the answers

What happens during the infiltration step in tissue preparation?

The tissue is impregnated with the embedding medium Signup and view all the answers