10 Questions
What is the typical thickness of paraffin sections for light microscopy?
3-10 μm
Which spatial unit is commonly used in microscopy that is equivalent to 10−4 μm?
Angstrom (Å)
How are frozen sections useful in histochemical studies compared to fixed tissues?
They do not dissolve cell lipids
Which dye behaves like a basic dye and stains basophilic tissue components?
Hematoxylin
What is the purpose of staining tissue sections in microscopy?
To make components distinguishable
What is the purpose of dehydrating fixed tissues before embedding?
To remove water from the tissue gradually
Why is embedding with plastic resin preferred over paraffin for some tissues?
Plastic resin embedding avoids tissue distortion due to lower required temperatures.
What is the purpose of the clearing step in tissue preparation?
To give the tissue a translucent appearance
Why is it important to trim the hardened block before sectioning?
To avoid damage to the microtome blade during sectioning
What happens during the infiltration step in tissue preparation?
The tissue is impregnated with the embedding medium
Learn about the process of infiltrating and embedding fixed tissues in materials like paraffin and plastic resins for microscopy. Understand the importance of dehydrating tissues prior to embedding through a series of ethanol solutions.
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