10 Questions
Paraffin Wax Manual – 4 ______ of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. Celloidin (Collodion) Wet – bones, teeth, large brain sections Dry – whole eye sections 3. Gelatin 4. Plastic PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4.
changes
Paraffin Wax Manual – 4 changes of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. ______ Wet – bones, teeth, large brain sections Dry – whole eye sections 3. Gelatin 4. Plastic PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4.
Celloidin (Collodion)
Paraffin Wax Manual – 4 changes of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. Celloidin (Collodion) Wet – bones, teeth, large brain sections Dry – whole eye sections 3. ______ 4. Plastic PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4.
Gelatin
Paraffin Wax Manual – 4 changes of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. Celloidin (Collodion) Wet – bones, teeth, large brain sections Dry – whole eye sections 3. Gelatin 4. ______ PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4.
Plastic
Paraffin Wax Manual – 4 changes of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. Celloidin (Collodion) Wet – bones, teeth, large brain sections Dry – whole eye sections 3. Gelatin 4. Plastic PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4.
Paraffin Wax Impregnation
Paraffin Wax Manual – 4 changes of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. Celloidin (Collodion) Wet – bones, teeth, large brain sections Dry – whole eye sections 3. ______ 4. Plastic PARAFFIN WAX IMPREGNATION
Gelatin
Paraffin Wax Manual – 4 changes of wax 15 mins interval Automatic – Autotechnicon, Elliott Bench type Vacuum – fastest, negative atmospheric pressure, gives the fastest result 2. Celloidin (Collodion) Wet – bones, teeth, large brain sections Dry – whole eye sections 3. Gelatin 4. ______ PARAFFIN WAX IMPREGNATION
Plastic
PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4. ______
Ester Wax
PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4. ______
Paraplast
PARAFFIN WAX IMPREGNATION Simplest, most common, best Melting point: 54c-58c at 20c-24c Ease in cutting Permanent paraffin blocks Good staining results Not recommended for fatty tissues Overheated paraffin brittle specimen Inadequate impregnation clearing agent retained Substitute for paraffin wax 1. Paraplast – more elastic / resilient (56-57C) Embeddol – less brittle (56-58C) Bioloid – for eyes Tissue Mat – with rubber 2. Ester Wax – low melting point (4. ______
Embeddol
Study Notes
Tissue Impregnation/Embedding
- Infiltration/impregnation involves replacing the clearing medium with a support medium that fills all tissue cavities.
Types of Tissue Impregnation/Embedding
- Paraffin wax: simplest, most common, and best method
- Celloidin (collodion): used for bones, teeth, large brain sections (wet), and whole eye sections (dry)
- Gelatin: another type of impregnation/embedding
- Plastic: also used for impregnation/embedding
Paraffin Wax Impregnation
- Melting point: 54°C-58°C at 20°C-24°C
- Advantages: ease in cutting, permanent paraffin blocks, and good staining results
- Disadvantages: not recommended for fatty tissues, overheated paraffin leads to brittle specimens, and inadequate impregnation retains clearing agent
Substitutes for Paraffin Wax
- Paraplast: more elastic/resilient (56°C-57°C)
- Embeddol: less brittle (56°C-58°C)
- Bioloid: used for eyes
- Tissue Mat: with rubber
- Ester Wax: low melting point
Test your knowledge of tissue impregnation and embedding techniques with this quiz. Explore the different types of impregnation methods, including paraffin wax manual, automatic, and vacuum processes. See how well you understand the process of permeating tissue with a support medium and the removal and replacement of clearing medium.
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