Tissue Embedding in Paraffin Wax

Tissue Embedding

• The purpose of rapid cooling the wax after tissue embedding is to prevent the formation of large wax crystals, which can lead to imperfect sections and compromise tissue morphology.

Avoiding Large Wax Crystals

• To avoid large wax crystals during tissue embedding, a slow and controlled cooling process should be avoided, as it allows crystals to form and grow.

Storing Paraffin Blocks

• Paraffin blocks should be stored in a cool, dry place, away from direct sunlight and protected from physical damage to prevent warping or cracking.

Excessive Cooling

• Excessive cooling during tissue embedding can lead to the formation of large, imperfect crystals, causing sections to be brittle and prone to tearing.

Specimen Orientation

• Specimen orientation is crucial during tissue embedding because it determines the plane of sectioning and affects the quality of the final histological sections; proper orientation ensures that diagnostic features are preserved and accurately represented.