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Questions and Answers
What is the primary purpose of transferring DNA onto a membrane in Southern blotting?
What is the primary purpose of transferring DNA onto a membrane in Southern blotting?
What is the role of probe in Southern blotting?
What is the role of probe in Southern blotting?
What determines the amount of DNA required for restriction enzyme digestion in Southern blotting?
What determines the amount of DNA required for restriction enzyme digestion in Southern blotting?
What is the purpose of DNA fragmentation using restriction endonucleases in Southern blotting?
What is the purpose of DNA fragmentation using restriction endonucleases in Southern blotting?
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Who invented Southern analysis, a key technique in DNA recombinant technology?
Who invented Southern analysis, a key technique in DNA recombinant technology?
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What is the purpose of using purine bases instead of pyrimidines during DNA treatment with diluted acid?
What is the purpose of using purine bases instead of pyrimidines during DNA treatment with diluted acid?
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Why is capillary action used for the transfer of DNA onto the membrane?
Why is capillary action used for the transfer of DNA onto the membrane?
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What is the role of 0.4 M NaOH in the transfer of DNA onto a nylon membrane?
What is the role of 0.4 M NaOH in the transfer of DNA onto a nylon membrane?
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Why is an alkaline buffer used for transferring DNA onto a nylon membrane?
Why is an alkaline buffer used for transferring DNA onto a nylon membrane?
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What is the main reason for using 20X SSC for transferring DNA onto a nitrocellulose membrane?
What is the main reason for using 20X SSC for transferring DNA onto a nitrocellulose membrane?
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