SDS-PAGE Electrophoresis Buffer
18 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the approximate pH of the electrophoresis buffer?

  • pH 8.8
  • pH 8.3 (correct)
  • pH 9.3
  • pH 7.8
  • What is the role of glycine in the electrophoresis buffer?

  • To provide a positive charge
  • To maintain a pH of 8.3
  • To provide a large amount of SDS
  • To set up a steep voltage gradient in the stacking gel (correct)
  • What is the primary effect of the chloride ions in the tank buffer and stacking gel?

  • To concentrate protein-SDS complexes
  • To slow down the migration of glycine molecules
  • To increase the voltage gradient in the running gel
  • To form a moving ion front (correct)
  • What is the purpose of the large pores in the stacking gel?

    <p>To concentrate protein-SDS complexes</p> Signup and view all the answers

    What happens when the glycine ions enter the running gel?

    <p>Dramatic changes occur</p> Signup and view all the answers

    What is the result of the difference in chloride and glycine mobility?

    <p>A steep voltage gradient in the stacking gel</p> Signup and view all the answers

    What is the effect of the alkaline pH of the running gel on glycine molecules?

    <p>They assume a negative charge</p> Signup and view all the answers

    What is the primary cause of proteins migrating at different rates in the running gel?

    <p>The sieving properties of the gel</p> Signup and view all the answers

    What is the purpose of pre-stained protein standards in electrophoresis?

    <p>To monitor the approximate positions of proteins during electrophoresis</p> Signup and view all the answers

    What is the effect of adding chromophores to a protein standard on the gel?

    <p>It produces more diffuse bands on the gel</p> Signup and view all the answers

    What is the purpose of rinsing the gel with water after electrophoresis?

    <p>To remove the buffer salts used for electrophoresis</p> Signup and view all the answers

    What is the characteristic of the stain Coomassie Brilliant Blue G-250?

    <p>It binds non-covalently and with very little specificity to proteins</p> Signup and view all the answers

    What is the main function of the electrophoresis buffer in SDS-PAGE?

    <p>To provide a consistent ionic strength and pH environment</p> Signup and view all the answers

    What are the two main components of the SDS-PAGE system?

    <p>Stacking and running gels</p> Signup and view all the answers

    What is the role of ammonium persulfate in the SDS-PAGE system?

    <p>To generate free oxygen radicals that catalyze the polymerization of acrylamide and bis-acrylamide</p> Signup and view all the answers

    What is the effect of increasing the concentration of acrylamide or bis-acrylamide in the gel matrix?

    <p>It decreases the pore size of the gel</p> Signup and view all the answers

    What is the function of glycerol in the SDS-PAGE system?

    <p>To allow protein samples to settle into the bottom of the gel wells</p> Signup and view all the answers

    What is the difference between the stacking and running gels?

    <p>They have different pore sizes, ionic strengths, and pHs</p> Signup and view all the answers

    More Like This

    SDS Page Principle
    18 questions

    SDS Page Principle

    FlashyPolonium avatar
    FlashyPolonium
    SDS-PAGE Biochemistry Quiz
    16 questions

    SDS-PAGE Biochemistry Quiz

    InexpensiveMoldavite2033 avatar
    InexpensiveMoldavite2033
    SDS-PAGE Techniques and Protocols
    16 questions

    SDS-PAGE Techniques and Protocols

    InexpensiveMoldavite2033 avatar
    InexpensiveMoldavite2033
    Use Quizgecko on...
    Browser
    Browser