18 Questions
What is the primary purpose of treating samples with protease inhibitor during protein extraction?
To prevent protein denaturation
What is the main difference between Southern blotting and Western blotting?
Target molecule being detected (DNA vs. protein)
What is the purpose of the blocking step in Western blotting?
To prevent non-specific binding
What is the primary function of the secondary antibody in Western blotting?
To enhance signal detection
What is the purpose of SDS-PAGE in Western blotting?
To separate proteins based on molecular weight
What is the mechanism of protein transfer from the gel to the membrane in Western blotting?
Electrotransference
What is the primary function of glycerol in the loading buffer during protein extraction?
To help sink the sample in the well
What is the driving force behind the transfer of proteins from the gel to the nitrocellulose membrane during electro-blotting?
Electric current
What is the primary purpose of blocking in western blotting?
To prevent non-specific binding of antibodies to the nitrocellulose membrane
What is the primary factor that determines the separation of proteins in SDS-PAGE?
Molecular weight and electric charge
What is the advantage of using electro-blotting over traditional blotting?
Reduced time required for protein transfer
What is the role of bromothymol blue in the sample preparation for SDS-PAGE?
To monitor the movement of proteins during electrophoresis
What is the primary function of the secondary antibody in a Western blotting procedure?
To recognize and bind to the primary antibody
Which of the following enzymes is NOT typically used as a label for the secondary antibody in Western blotting?
Lactate dehydrogenase
What is the purpose of incubating the reaction mixture with a specific substrate in Western blotting?
To visualize the enzyme action
What is a primary application of Western blotting in disease diagnosis?
Detection of antibodies against viruses or bacteria in serum
What is the confirmatory test for HIV that utilizes Western blotting?
Detection of anti-HIV antibodies in patient's serum
What is the type of protein that can be detected using Western blotting?
All types of proteins
Learn about the process of SDS-PAGE gel electrophoresis, including sample preparation and protein separation. Understand how tracking dyes and loading buffers work in this lab technique. Test your knowledge of protein analysis!
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