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SDS Page Principle

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Questions and Answers

What is the primary reason for protein migration towards the anode in an electric field?

Due to the protein's shape

Due to the protein's size

Due to the protein's molecular weight

Due to the protein's charge (correct)

What is the purpose of the molecular weight ladder in protein visualization?

To determine the protein's size (correct)

To calculate the protein's migration distance

To separate the proteins based on their molecular weight

To determine the protein's charge

What is the term for the relative mobility of charged species in an electric field?

Electrophoretic mobility

Relative mobility (correct)

Separation coefficient

Frictional resistance

What is the purpose of the stacking gel in protein visualization?

To concentrate the proteins into a thin layer Signup and view all the answers

What determines the direction of movement of a charged molecule in an electric field?

The sign of the charge Signup and view all the answers

What is the equation that relates the relative mobility of charged species to the charge, voltage, and frictional resistance?

Rf = ZE/F Signup and view all the answers

What is the primary mechanism by which proteins separate during SDS PAGE?

By molecular mass through the sieving effect of pores Signup and view all the answers

What is the purpose of wearing gloves during SDS PAGE?

To handle the neurotoxin acrylamide safely Signup and view all the answers

What is the composition of the 30% polyacrylamide solution?

29g acrylamide + 1g bis-acrylamide in 50mL of water Signup and view all the answers

What is the purpose of the casting frame in SDS PAGE?

To cast the gel Signup and view all the answers

What is the function of the 10% SDS solution?

To denature the proteins Signup and view all the answers

What is the pH of the 1.5M Tris solution?

8.8 Signup and view all the answers

What is the primary factor that determines the electrophoretic mobility of biological molecules in a general electrophoresis technique?

Charge and size Signup and view all the answers

What is the purpose of treating biological samples with SDS in polyacrylamide gel electrophoresis?

To denature the proteins Signup and view all the answers

What is the expected outcome when a protein mixture is separated using PAGE?

Proteins are separated based on their size and charge Signup and view all the answers

What is the reason for observing a highly resolved high molecular weight band when genomic DNA is exposed to UV light?

Good quality of DNA Signup and view all the answers

What is the purpose of exposing the gel to UV light in the experiment?

To confirm the quality of DNA Signup and view all the answers

What is the expected outcome when genomic DNA is run on a gel and exposed to UV light?

RNA is settled at the bottom and DNA is observed at the top Signup and view all the answers