Podcast
Questions and Answers
What is the primary reason for protein migration towards the anode in an electric field?
What is the primary reason for protein migration towards the anode in an electric field?
- Due to the protein's shape
- Due to the protein's size
- Due to the protein's molecular weight
- Due to the protein's charge (correct)
What is the purpose of the molecular weight ladder in protein visualization?
What is the purpose of the molecular weight ladder in protein visualization?
- To determine the protein's size (correct)
- To calculate the protein's migration distance
- To separate the proteins based on their molecular weight
- To determine the protein's charge
What is the term for the relative mobility of charged species in an electric field?
What is the term for the relative mobility of charged species in an electric field?
- Electrophoretic mobility
- Relative mobility (correct)
- Separation coefficient
- Frictional resistance
What is the purpose of the stacking gel in protein visualization?
What is the purpose of the stacking gel in protein visualization?
What determines the direction of movement of a charged molecule in an electric field?
What determines the direction of movement of a charged molecule in an electric field?
What is the equation that relates the relative mobility of charged species to the charge, voltage, and frictional resistance?
What is the equation that relates the relative mobility of charged species to the charge, voltage, and frictional resistance?
What is the primary mechanism by which proteins separate during SDS PAGE?
What is the primary mechanism by which proteins separate during SDS PAGE?
What is the purpose of wearing gloves during SDS PAGE?
What is the purpose of wearing gloves during SDS PAGE?
What is the composition of the 30% polyacrylamide solution?
What is the composition of the 30% polyacrylamide solution?
What is the purpose of the casting frame in SDS PAGE?
What is the purpose of the casting frame in SDS PAGE?
What is the function of the 10% SDS solution?
What is the function of the 10% SDS solution?
What is the pH of the 1.5M Tris solution?
What is the pH of the 1.5M Tris solution?
What is the primary factor that determines the electrophoretic mobility of biological molecules in a general electrophoresis technique?
What is the primary factor that determines the electrophoretic mobility of biological molecules in a general electrophoresis technique?
What is the purpose of treating biological samples with SDS in polyacrylamide gel electrophoresis?
What is the purpose of treating biological samples with SDS in polyacrylamide gel electrophoresis?
What is the expected outcome when a protein mixture is separated using PAGE?
What is the expected outcome when a protein mixture is separated using PAGE?
What is the reason for observing a highly resolved high molecular weight band when genomic DNA is exposed to UV light?
What is the reason for observing a highly resolved high molecular weight band when genomic DNA is exposed to UV light?
What is the purpose of exposing the gel to UV light in the experiment?
What is the purpose of exposing the gel to UV light in the experiment?
What is the expected outcome when genomic DNA is run on a gel and exposed to UV light?
What is the expected outcome when genomic DNA is run on a gel and exposed to UV light?
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