18 Questions
What is the primary reason for protein migration towards the anode in an electric field?
Due to the protein's charge
What is the purpose of the molecular weight ladder in protein visualization?
To determine the protein's size
What is the term for the relative mobility of charged species in an electric field?
Relative mobility
What is the purpose of the stacking gel in protein visualization?
To concentrate the proteins into a thin layer
What determines the direction of movement of a charged molecule in an electric field?
The sign of the charge
What is the equation that relates the relative mobility of charged species to the charge, voltage, and frictional resistance?
Rf = ZE/F
What is the primary mechanism by which proteins separate during SDS PAGE?
By molecular mass through the sieving effect of pores
What is the purpose of wearing gloves during SDS PAGE?
To handle the neurotoxin acrylamide safely
What is the composition of the 30% polyacrylamide solution?
29g acrylamide + 1g bis-acrylamide in 50mL of water
What is the purpose of the casting frame in SDS PAGE?
To cast the gel
What is the function of the 10% SDS solution?
To denature the proteins
What is the pH of the 1.5M Tris solution?
8.8
What is the primary factor that determines the electrophoretic mobility of biological molecules in a general electrophoresis technique?
Charge and size
What is the purpose of treating biological samples with SDS in polyacrylamide gel electrophoresis?
To denature the proteins
What is the expected outcome when a protein mixture is separated using PAGE?
Proteins are separated based on their size and charge
What is the reason for observing a highly resolved high molecular weight band when genomic DNA is exposed to UV light?
Good quality of DNA
What is the purpose of exposing the gel to UV light in the experiment?
To confirm the quality of DNA
What is the expected outcome when genomic DNA is run on a gel and exposed to UV light?
RNA is settled at the bottom and DNA is observed at the top
Learn about the principle behind SDS page, a laboratory technique used to separate proteins based on their size and charge. Understand how proteins migrate in an electric field and how their size is calculated.
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