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### What are the three main factors contributing to zone broadening?

Longitudinal diffusion, resistance to mass transfer, multiple path effect

### What is the equation for band broadening as a result of longitudinal diffusion?

$HL = \frac{L1 \times DM}{V}$

### In which situation is longitudinal diffusion not very important in liquid chromatography?

At high flow rates

### What does the degree of band broadening in longitudinal diffusion depend on?

<p>Diffusion of the solute and the flow rate of the solute through the column</p> Signup and view all the answers

### What contributes to a decrease in band broadening as a result of longitudinal diffusion?

<p>Increase in velocity of mobile phase</p> Signup and view all the answers

## Study Notes

### Factors Contributing to Zone Broadening

• Three main factors contribute to zone broadening: longitudinal diffusion, eddy diffusion, and mass transfer

### Longitudinal Diffusion

• Band broadening as a result of longitudinal diffusion is described by the equation: σ² = 2DT
• Where σ² is the variance, D is the diffusion coefficient, and T is time
• Longitudinal diffusion is not very important in liquid chromatography when the flow rate is high
• The degree of band broadening in longitudinal diffusion depends on the diffusion coefficient of the analyte, the time spent in the column, and the flow rate
• A decrease in band broadening as a result of longitudinal diffusion is contributed by a high flow rate and a low diffusion coefficient of the analyte

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## Description

Test your knowledge on the factors contributing to zone broadening in rate theory. Learn about longitudinal diffusion, resistance to mass transfer, and the multiple path effect (Eddy diffusion).

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