Podcast Beta
Questions and Answers
What are the three main factors contributing to zone broadening?
What is the equation for band broadening as a result of longitudinal diffusion?
In which situation is longitudinal diffusion not very important in liquid chromatography?
What does the degree of band broadening in longitudinal diffusion depend on?
Signup and view all the answers
What contributes to a decrease in band broadening as a result of longitudinal diffusion?
Signup and view all the answers
Study Notes
Factors Contributing to Zone Broadening
- Three main factors contribute to zone broadening: longitudinal diffusion, eddy diffusion, and mass transfer
Longitudinal Diffusion
- Band broadening as a result of longitudinal diffusion is described by the equation: σ² = 2DT
- Where σ² is the variance, D is the diffusion coefficient, and T is time
- Longitudinal diffusion is not very important in liquid chromatography when the flow rate is high
- The degree of band broadening in longitudinal diffusion depends on the diffusion coefficient of the analyte, the time spent in the column, and the flow rate
- A decrease in band broadening as a result of longitudinal diffusion is contributed by a high flow rate and a low diffusion coefficient of the analyte
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Description
Test your knowledge on the factors contributing to zone broadening in rate theory. Learn about longitudinal diffusion, resistance to mass transfer, and the multiple path effect (Eddy diffusion).