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Questions and Answers
What are the three main factors contributing to zone broadening?
What are the three main factors contributing to zone broadening?
- Column length, column diameter, sample concentration
- Longitudinal diffusion, resistance to mass transfer, multiple path effect (correct)
- Temperature, pressure, flow rate
- Mobile phase composition, stationary phase composition, solute size
What is the equation for band broadening as a result of longitudinal diffusion?
What is the equation for band broadening as a result of longitudinal diffusion?
- $HL = \frac{DM}{V}$
- $HL = \frac{V \times DM}{L1}$
- $HL = \frac{L1 \times DM}{V}$ (correct)
- $HL = L1 \times V \times DM$
In which situation is longitudinal diffusion not very important in liquid chromatography?
In which situation is longitudinal diffusion not very important in liquid chromatography?
- At high flow rates (correct)
- At low temperature
- At high temperature
- At low flow rates
What does the degree of band broadening in longitudinal diffusion depend on?
What does the degree of band broadening in longitudinal diffusion depend on?
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What contributes to a decrease in band broadening as a result of longitudinal diffusion?
What contributes to a decrease in band broadening as a result of longitudinal diffusion?
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Study Notes
Factors Contributing to Zone Broadening
- Three main factors contribute to zone broadening: longitudinal diffusion, eddy diffusion, and mass transfer
Longitudinal Diffusion
- Band broadening as a result of longitudinal diffusion is described by the equation: σ² = 2DT
- Where σ² is the variance, D is the diffusion coefficient, and T is time
- Longitudinal diffusion is not very important in liquid chromatography when the flow rate is high
- The degree of band broadening in longitudinal diffusion depends on the diffusion coefficient of the analyte, the time spent in the column, and the flow rate
- A decrease in band broadening as a result of longitudinal diffusion is contributed by a high flow rate and a low diffusion coefficient of the analyte
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