5 Questions
What are the three main factors contributing to zone broadening?
Longitudinal diffusion, resistance to mass transfer, multiple path effect
What is the equation for band broadening as a result of longitudinal diffusion?
$HL = \frac{L1 \times DM}{V}$
In which situation is longitudinal diffusion not very important in liquid chromatography?
At high flow rates
What does the degree of band broadening in longitudinal diffusion depend on?
Diffusion of the solute and the flow rate of the solute through the column
What contributes to a decrease in band broadening as a result of longitudinal diffusion?
Increase in velocity of mobile phase
Study Notes
Factors Contributing to Zone Broadening
- Three main factors contribute to zone broadening: longitudinal diffusion, eddy diffusion, and mass transfer
Longitudinal Diffusion
- Band broadening as a result of longitudinal diffusion is described by the equation: σ² = 2DT
- Where σ² is the variance, D is the diffusion coefficient, and T is time
- Longitudinal diffusion is not very important in liquid chromatography when the flow rate is high
- The degree of band broadening in longitudinal diffusion depends on the diffusion coefficient of the analyte, the time spent in the column, and the flow rate
- A decrease in band broadening as a result of longitudinal diffusion is contributed by a high flow rate and a low diffusion coefficient of the analyte
Test your knowledge on the factors contributing to zone broadening in rate theory. Learn about longitudinal diffusion, resistance to mass transfer, and the multiple path effect (Eddy diffusion).
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