Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
What contributes to zone broadening in chromatography?
What contributes to zone broadening in chromatography?
- Solute concentration, column length, flow rate
- Transverse diffusion, heat transfer, pressure effect
- Temperature, solvent type, column material
- Longitudinal diffusion, resistance to mass transfer, multiple path effect (correct)
What is the formula for band broadening due to longitudinal diffusion?
What is the formula for band broadening due to longitudinal diffusion?
- $HL = \frac{L1}{DMV}$
- $HL = \frac{L1}{DM} \times V$
- $HL = L1 + DM - V$
- $HL = L1 \frac{DM}{V}$ (correct)
When is longitudinal diffusion most important in liquid chromatography?
When is longitudinal diffusion most important in liquid chromatography?
- When using a short column
- At low flow rates (correct)
- At high flow rates
- When using a long column
How does an increase in velocity of the mobile phase affect band broadening due to longitudinal diffusion?
How does an increase in velocity of the mobile phase affect band broadening due to longitudinal diffusion?
Signup and view all the answers
What does the band broadening due to longitudinal diffusion depend on?
What does the band broadening due to longitudinal diffusion depend on?
Signup and view all the answers
Light waves consist of perpendicular and oscillating electric and magnetic fields.
Light waves consist of perpendicular and oscillating electric and magnetic fields.
Signup and view all the answers
The frequency of light waves is measured in cycles per second or Hertz (Hz).
The frequency of light waves is measured in cycles per second or Hertz (Hz).
Signup and view all the answers
Each photon of electromagnetic radiation carries the energy denoted by E=hυ.
Each photon of electromagnetic radiation carries the energy denoted by E=hυ.
Signup and view all the answers
Wavenumber measures the number of waves per centimeter.
Wavenumber measures the number of waves per centimeter.
Signup and view all the answers
The Planck's constant is approximately 6.6 x 10^-34 Joule.
The Planck's constant is approximately 6.6 x 10^-34 Joule.
Signup and view all the answers
