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Lecture 5: Rate Theory Bond Broadening

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Questions and Answers

What are the three main factors contributing to zone broadening?

Longitudinal diffusion, transverse diffusion, eddy current effect

Eddy current effect, longitudinal dispersion, transverse diffusion

Transverse diffusion, mass transfer resistance, longitudinal dispersion

Longitudinal diffusion, resistance to mass transfer, multiple path effect (correct)

What does the band broadening due to longitudinal diffusion depend on?

Viscosity of the stationary phase

Diffusion of the solute and flow rate of the solute through the column (correct)

Concentration of the solute in the mobile phase

Temperature and pressure of the mobile phase

In what way does the velocity of the mobile phase affect band broadening due to longitudinal diffusion?

Band broadening is directly proportional to the velocity of mobile phase

As velocity of mobile phase increases, the band broadening as a result of longitudinal diffusion increases

As velocity of mobile phase increases, the band broadening as a result of longitudinal diffusion decreases (correct)

Velocity of mobile phase has no effect on band broadening

What is the formula for band broadening as a result of longitudinal diffusion?

<p>$HL = \frac{L1 DM}{V}$ (C)</p> Signup and view all the answers

When is longitudinal diffusion considered important in liquid chromatography?

<p>At low flow rates (B)</p> Signup and view all the answers