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Questions and Answers
What are the three main factors contributing to zone broadening?
What are the three main factors contributing to zone broadening?
- Longitudinal diffusion, transverse diffusion, eddy current effect
- Eddy current effect, longitudinal dispersion, transverse diffusion
- Transverse diffusion, mass transfer resistance, longitudinal dispersion
- Longitudinal diffusion, resistance to mass transfer, multiple path effect (correct)
What does the band broadening due to longitudinal diffusion depend on?
What does the band broadening due to longitudinal diffusion depend on?
- Viscosity of the stationary phase
- Diffusion of the solute and flow rate of the solute through the column (correct)
- Concentration of the solute in the mobile phase
- Temperature and pressure of the mobile phase
In what way does the velocity of the mobile phase affect band broadening due to longitudinal diffusion?
In what way does the velocity of the mobile phase affect band broadening due to longitudinal diffusion?
- Band broadening is directly proportional to the velocity of mobile phase
- As velocity of mobile phase increases, the band broadening as a result of longitudinal diffusion increases
- As velocity of mobile phase increases, the band broadening as a result of longitudinal diffusion decreases (correct)
- Velocity of mobile phase has no effect on band broadening
What is the formula for band broadening as a result of longitudinal diffusion?
What is the formula for band broadening as a result of longitudinal diffusion?
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When is longitudinal diffusion considered important in liquid chromatography?
When is longitudinal diffusion considered important in liquid chromatography?
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