5 Questions
What are the three main factors contributing to zone broadening?
Longitudinal diffusion, resistance to mass transfer, multiple path effect
What does the band broadening due to longitudinal diffusion depend on?
Diffusion of the solute and flow rate of the solute through the column
In what way does the velocity of the mobile phase affect band broadening due to longitudinal diffusion?
As velocity of mobile phase increases, the band broadening as a result of longitudinal diffusion decreases
What is the formula for band broadening as a result of longitudinal diffusion?
$HL = \frac{L1 DM}{V}$
When is longitudinal diffusion considered important in liquid chromatography?
At low flow rates
Explore the factors contributing to zone broadening in rate theory, including longitudinal diffusion, resistance to mass transfer, and the multiple path effect. Understand how average migration rates for molecules of the same solute are not identical due to specific solute characteristics.
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