10 Questions
What are the three main factors contributing to zone broadening?
Longitudinal diffusion, resistance to mass transfer, multiple path effect
What does the degree of band broadening in longitudinal diffusion depend on?
Diffusion of the solute and the flow rate of the solute through the column
What does HL represent in the equation HL = L1 DM / V?
Band broadening as a result of longitudinal diffusion
When is longitudinal diffusion not very important in liquid chromatography?
At low flow rates
What does resistance to mass transfer or mass transfer diffusion refer to?
Mass transfer of solute in mobile or stationary phases
What does the band broadening as a result of longitudinal diffusion depend on?
Diffusion of the solute and the flow rate of the solute through the column
When is longitudinal diffusion considered not very important in liquid chromatography?
At low flow rates
What does resistance to mass transfer or mass transfer diffusion refer to?
The hindrance encountered by solute molecules in moving from the stationary phase to the mobile phase
What is the equation representing band broadening as a result of longitudinal diffusion?
$HL = L1 \frac{DM}{V}$
3
Study Notes
Factors Contributing to Zone Broadening
- Three main factors contribute to zone broadening: longitudinal diffusion, eddy diffusion, and resistance to mass transfer
Longitudinal Diffusion
- Degree of band broadening in longitudinal diffusion depends on the ratio of column length to particle diameter
- Longitudinal diffusion is not very important in liquid chromatography when the flow rate is high or the column is packed with small particles
- Band broadening as a result of longitudinal diffusion depends on the column length, particle diameter, and diffusion coefficient
Equation and Terminology
- HL = L1 DM / V represents the equation for band broadening as a result of longitudinal diffusion
- HL represents the height equivalent to a theoretical plate
- Resistance to mass transfer or mass transfer diffusion refers to the hindrance to the solute molecules' movement in the column
Test your knowledge on rate theory and bond broadening with this quiz. Learn about the factors affecting zone broadening and the behavior of molecules in the context of solutes and diffusion.
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