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Questions and Answers

What contributes to zone broadening in chromatography?

  • Longitudinal diffusion, solute size, and mobile phase viscosity
  • Longitudinal diffusion, temperature variation, and column length
  • Longitudinal diffusion, resistance to mass transfer, and multiple path effect (correct)
  • Resistance to mass transfer, solute concentration, and flow rate
  • What does the band broadening due to longitudinal diffusion depend on?

  • Column diameter and solute concentration
  • Temperature and pressure of the mobile phase
  • Mobile phase viscosity and stationary phase mass transfer
  • Diffusion of the solute and the flow rate of the solute through the column (correct)
  • When is longitudinal diffusion not very important in liquid chromatography?

  • At high flow rates
  • At low flow rates (correct)
  • When using a high concentration of solute
  • When using a narrow column
  • What does the resistance to mass transfer or mass transfer diffusion involve?

    <p>Mass transfer of solute in mobile or stationary phases</p> Signup and view all the answers

    How does an increase in velocity of the mobile phase affect band broadening due to longitudinal diffusion?

    <p>It decreases band broadening as a result of longitudinal diffusion</p> Signup and view all the answers

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