Podcast
Questions and Answers
What contributes to zone broadening in chromatography?
What contributes to zone broadening in chromatography?
- Longitudinal diffusion, solute size, and mobile phase viscosity
- Longitudinal diffusion, temperature variation, and column length
- Longitudinal diffusion, resistance to mass transfer, and multiple path effect (correct)
- Resistance to mass transfer, solute concentration, and flow rate
What does the band broadening due to longitudinal diffusion depend on?
What does the band broadening due to longitudinal diffusion depend on?
- Column diameter and solute concentration
- Temperature and pressure of the mobile phase
- Mobile phase viscosity and stationary phase mass transfer
- Diffusion of the solute and the flow rate of the solute through the column (correct)
When is longitudinal diffusion not very important in liquid chromatography?
When is longitudinal diffusion not very important in liquid chromatography?
- At high flow rates
- At low flow rates (correct)
- When using a high concentration of solute
- When using a narrow column
What does the resistance to mass transfer or mass transfer diffusion involve?
What does the resistance to mass transfer or mass transfer diffusion involve?
How does an increase in velocity of the mobile phase affect band broadening due to longitudinal diffusion?
How does an increase in velocity of the mobile phase affect band broadening due to longitudinal diffusion?