Protoplast Transformation Methods Quiz
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Questions and Answers

What is one key advantage of using protoplasts for gene transfer in higher plants?

  • Protoplasts are resistant to stress during the gene transfer process
  • Protoplasts are easier to genetically modify compared to other plant cells
  • Protoplasts can be cultured and lead to the regeneration of whole plants (correct)
  • Protoplasts have limited diversity in terms of genotypes
  • Why is it essential to develop a variety of techniques for gene transfer in plants?

  • To limit the diversity of genotypes within plant species
  • To overcome the difficulty of identifying target cells for gene transformation
  • To cater to the enormous diversity of plant species and genotypes available (correct)
  • To simplify the process of gene transfer in plants
  • What distinguishes plant cells from animal cells in terms of regeneration potential?

  • Animal cells are totipotent and can regenerate more efficiently than plant cells
  • Animal cells do not require in vitro conditions for regeneration
  • Plant cells are totipotent and can be stimulated to regenerate into whole plants (correct)
  • Plant cells are incapable of regeneration into whole plants
  • Why is genome stress mentioned as a concern in in vitro plant regeneration?

    <p>Genome stress is a result of in vitro plant regeneration via callus phase</p> Signup and view all the answers

    Why are transformed cells that cannot undergo regeneration considered of limited value?

    <p>Because they cannot give rise to whole transformed plants</p> Signup and view all the answers

    What factor makes the identification of target cell types for gene transformation challenging in many plant species?

    <p>'Plant cell totipotency' that makes all cells potential target types</p> Signup and view all the answers

    How do immature embryos, pollen, and zygotes contribute to gene transfer in plants?

    <p>They can be used as alternative sources for gene transfer in plants</p> Signup and view all the answers

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