7 Questions
What is one key advantage of using protoplasts for gene transfer in higher plants?
Protoplasts can be cultured and lead to the regeneration of whole plants
Why is it essential to develop a variety of techniques for gene transfer in plants?
To cater to the enormous diversity of plant species and genotypes available
What distinguishes plant cells from animal cells in terms of regeneration potential?
Plant cells are totipotent and can be stimulated to regenerate into whole plants
Why is genome stress mentioned as a concern in in vitro plant regeneration?
Genome stress is a result of in vitro plant regeneration via callus phase
Why are transformed cells that cannot undergo regeneration considered of limited value?
Because they cannot give rise to whole transformed plants
What factor makes the identification of target cell types for gene transformation challenging in many plant species?
'Plant cell totipotency' that makes all cells potential target types
How do immature embryos, pollen, and zygotes contribute to gene transfer in plants?
They can be used as alternative sources for gene transfer in plants
Test your knowledge on various methods used for transforming protoplasts, such as the holding pipette method and electroporation. Learn about isolating protoplasts, injecting DNA into the nucleus, and culturing injected cells.
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