Protoplast Microinjection Methods Quiz
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Questions and Answers

What is a main disadvantage of the technique that involves the production of chimeric plants?

  • Slow process
  • Requirement of unskilled personnel
  • High cost
  • Production of plants with only a part transformed (correct)

What are the alternate cellular targets for transformation mentioned in the text?

  • Seeds, fruits, flowers
  • Immature embryos, meristems, immature pollen (correct)
  • Mature embryos, leaves, mature pollen
  • Roots, flowers, stems

What is used to transfer macromolecules into the cytoplasm or nucleus of a recipient cell in microinjection?

  • Stainless steel rods
  • Plastic tubes
  • Metal wires
  • Glass micropipettes with 0.5-10μm diameter tip (correct)

What is employed for microinjecting DNA in the microinjection technique?

<p>Specially designed micromanipulator (A)</p> Signup and view all the answers

Which plant species have transgenic chimeras been obtained in using the microinjection method?

<p>Oilseed rape (Brassica napus) (D)</p> Signup and view all the answers

What type of cells or protoplasts are used as targets in the microinjection technique?

<p>Cells immobilized on a solid support (B)</p> Signup and view all the answers

Why is the hanging droplet culture method not specifically mentioned in the text?

<p>'Hanging droplet culture' is not part of the microinjection or macroinjection techniques discussed. (A)</p> Signup and view all the answers

'Transformed plants of single cell origin' can be obtained from:

<p>'Chimeric plants' (A)</p> Signup and view all the answers

'High rate of success' in microinjection comes at the cost of:

<p>'Highly skilled and experienced personnel' (D)</p> Signup and view all the answers

'Expensive' is a characteristic associated with the technique due to its:

<p>'Costly materials and resources' (A)</p> Signup and view all the answers

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