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Protoplast Microinjection Methods Quiz

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Questions and Answers

What is a main disadvantage of the technique that involves the production of chimeric plants?

Slow process

Requirement of unskilled personnel

High cost

Production of plants with only a part transformed (correct)

What are the alternate cellular targets for transformation mentioned in the text?

Seeds, fruits, flowers

Immature embryos, meristems, immature pollen (correct)

Mature embryos, leaves, mature pollen

Roots, flowers, stems

What is used to transfer macromolecules into the cytoplasm or nucleus of a recipient cell in microinjection?

Stainless steel rods

Plastic tubes

Metal wires

Glass micropipettes with 0.5-10μm diameter tip (correct)

What is employed for microinjecting DNA in the microinjection technique?

Specially designed micromanipulator Signup and view all the answers

Which plant species have transgenic chimeras been obtained in using the microinjection method?

Oilseed rape (Brassica napus) Signup and view all the answers

What type of cells or protoplasts are used as targets in the microinjection technique?

Cells immobilized on a solid support Signup and view all the answers

Why is the hanging droplet culture method not specifically mentioned in the text?

'Hanging droplet culture' is not part of the microinjection or macroinjection techniques discussed. Signup and view all the answers

'Transformed plants of single cell origin' can be obtained from:

'Chimeric plants' Signup and view all the answers

'High rate of success' in microinjection comes at the cost of:

'Highly skilled and experienced personnel' Signup and view all the answers

'Expensive' is a characteristic associated with the technique due to its:

'Costly materials and resources' Signup and view all the answers