10 Questions
What is a main disadvantage of the technique that involves the production of chimeric plants?
Production of plants with only a part transformed
What are the alternate cellular targets for transformation mentioned in the text?
Immature embryos, meristems, immature pollen
What is used to transfer macromolecules into the cytoplasm or nucleus of a recipient cell in microinjection?
Glass micropipettes with 0.5-10μm diameter tip
What is employed for microinjecting DNA in the microinjection technique?
Specially designed micromanipulator
Which plant species have transgenic chimeras been obtained in using the microinjection method?
Oilseed rape (Brassica napus)
What type of cells or protoplasts are used as targets in the microinjection technique?
Cells immobilized on a solid support
Why is the hanging droplet culture method not specifically mentioned in the text?
'Hanging droplet culture' is not part of the microinjection or macroinjection techniques discussed.
'Transformed plants of single cell origin' can be obtained from:
'Chimeric plants'
'High rate of success' in microinjection comes at the cost of:
'Highly skilled and experienced personnel'
'Expensive' is a characteristic associated with the technique due to its:
'Costly materials and resources'
Test your knowledge on the various methods used for microinjecting protoplasts, including the holding pipette method and electroporation. Learn about isolating protoplasts, injecting DNA into the nucleus, and culturing injected cells.
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