Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
What is the main purpose of liposome mediated gene transfer?
What is the main purpose of liposome mediated gene transfer?
- Transferring single-stranded DNA between live bacteria
- Introducing foreign DNA into bacterial cells
- Enclosing plasmids within lipid bags for gene transfer (correct)
- Enhancing the transformation efficiency of E. Coli
What is the role of CaCl2 in the transformation method used for introducing foreign DNA into E. Coli?
What is the role of CaCl2 in the transformation method used for introducing foreign DNA into E. Coli?
- Breaking the cell wall at certain regions and binding DNA to the cell surface (correct)
- Facilitating the cytoplasmic bridges formation between live bacteria
- Promoting the heat shock treatment at 37-45°C
- Enhancing the uptake of plasmid DNA by E. Coli
What is the key feature of electroporation technique in gene transfer?
What is the key feature of electroporation technique in gene transfer?
- Transferring DNA via cytoplasmic bridges from donor to recipient bacteria
- Applying ultrasonic pulse generator for DNA transfer
- Creating temporary pores in cell membranes using electric pulses (correct)
- Using lipid bags to enclose plasmids
In lipofection, how are liposomes induced to fuse with protoplasts for gene transfer?
In lipofection, how are liposomes induced to fuse with protoplasts for gene transfer?
Signup and view all the answers
What defines the transformation frequency in the method used for introducing foreign DNA into bacterial cells?
What defines the transformation frequency in the method used for introducing foreign DNA into bacterial cells?
Signup and view all the answers
Flashcards are hidden until you start studying
