Protoplast Genetic Transformation Methods Quiz
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Questions and Answers

What is the main purpose of liposome mediated gene transfer?

  • Transferring single-stranded DNA between live bacteria
  • Introducing foreign DNA into bacterial cells
  • Enclosing plasmids within lipid bags for gene transfer (correct)
  • Enhancing the transformation efficiency of E. Coli

    • What is the role of CaCl2 in the transformation method used for introducing foreign DNA into E. Coli?

  • Breaking the cell wall at certain regions and binding DNA to the cell surface (correct)
  • Facilitating the cytoplasmic bridges formation between live bacteria
  • Promoting the heat shock treatment at 37-45°C
  • Enhancing the uptake of plasmid DNA by E. Coli

    • What is the key feature of electroporation technique in gene transfer?

  • Transferring DNA via cytoplasmic bridges from donor to recipient bacteria
  • Applying ultrasonic pulse generator for DNA transfer
  • Creating temporary pores in cell membranes using electric pulses (correct)
  • Using lipid bags to enclose plasmids

    • In lipofection, how are liposomes induced to fuse with protoplasts for gene transfer?

    <p>By treating with PEG and employing heat shock treatment</p> Signup and view all the answers

    What defines the transformation frequency in the method used for introducing foreign DNA into bacterial cells?

    <p>The fraction of cell population that can be transferred</p> Signup and view all the answers

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