Questions and Answers
What does an antiglycolytic agent do?
Prevent the breakdown of glucose
This needle is the standard needle for routine venipuncture.
21 gauge
Which of the following tubes contains an anticoagulant that works by binding calcium?
What is the purpose of a royal blue topped tube?
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Needles are color-coded according to:
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EDTA is a (n):
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Which of the following would be the best choice of equipment for drawing a small hand vein?
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Which anticoagulant prevents coagulation by inhibiting thrombin formation?
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Which of the following substances would be the best thing to use to disinfect a blood spill on a lab counter-top prior to cleanup?
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A light blue topped tube is most often associated with tests in which department?
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What causes evacuated tubes to fill with blood automatically?
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When should additive tubes be mixed?
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Which evacuated tube is suitable for blood culture collection?
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Which of the following additives is not an anticoagulant?
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You are performing a vein puncture on a difficult vein using a butterfly. You have an SST (red/tiger top) and light blue topped tube to collect. How do you proceed?
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All of the following chemicals are used as antiseptics EXCEPT:
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The additive in the lavender tube is:
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According to the current CLSI/NCCLS guidelines, the correct order of draw when using the evacuated tube system is:
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Which of the following would compromise the quality of the specimen received for testing?
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Why should a multi-sample needle with a broken seal not be used to draw blood?
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Which of the following is NOT a desirable characteristic of a safety feature according to the FDA?
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Which evacuated tube must be filled to the correct level in order to maintain a 9:1 ratio of the blood to anticoagulant?
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Tourniquets contaminated with visible blood should be:
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What lab tests are performed using EDTA (lavender top tubes)?
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What lab tests are performed using light blue (sodium citrate)?
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Tubes containing anticoagulants should be inverted immediately after filling to:
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What does it mean by a 'short draw'?
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Study Notes
Antiglycolytic Agents
- Antiglycolytic agents prevent the breakdown of glucose in blood samples.
Venipuncture Needles
- The standard needle for routine venipuncture is 21 gauge.
- Smaller 23-gauge needles are preferred for drawing from small hand veins.
Blood Collection Tubes
- Light blue top tubes contain an anticoagulant that binds calcium, essential for coagulation tests.
- Royal blue topped tubes are designed to minimize trace-element contamination.
- The yellow top tube (SPS) is used for blood culture collections.
Anticoagulants and Additives
- EDTA is a common anticoagulant used in lavender top tubes for CBC tests.
- Thrombin is not an anticoagulant.
- Heparin prevents coagulation by inhibiting thrombin formation.
Disinfecting and Handling Samples
- A 1:10 bleach solution is recommended for disinfecting blood spills on lab surfaces.
- Additive tubes should be mixed immediately after removal from the tube holder to prevent microclot formation.
- Partial filling of sodium citrate tubes can compromise specimen quality.
Order of Draw
- The correct order of draw for evacuated tubes is: blue, red, SST, green, lavender.
- The light blue topped tube must be filled to the correct level to maintain a 9:1 blood to anticoagulant ratio.
Safety and Disposal
- Multi-sample needles with a broken seal should not be used as they are not sterile.
- Contaminated tourniquets should be disposed of in biohazard bags.
- Safety features of devices must be attached and not considered accessories.
Mixing Requirements
- Proper mixing of tubes with additives is crucial to avoid complications such as microclots.
Common Lab Tests
- Light blue topped tubes are primarily used for PT/INR tests, while lavender top tubes are used for CBC tests.
Studying That Suits You
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Description
Test your knowledge with these flashcards covering key concepts from Chapter 7 of Phlebotomy. Learn about antiglycolytic agents, standard venipuncture needles, and anticoagulant tubes. Perfect for students preparing for exams!
