Podcast
Questions and Answers
What is the purpose of treating the RNA solution with alcohol and concentrated acid in the isolation process?
What is the purpose of treating the RNA solution with alcohol and concentrated acid in the isolation process?
- To inactivate RNA
- To prevent nuclease degradation
- To remove associated proteins and interfering substances (correct)
- To dissolve RNA into a solution
Which property of nucleic acids are chemical tests based on, as mentioned in the text?
Which property of nucleic acids are chemical tests based on, as mentioned in the text?
- Solubility
- Acidity
- Phosphorus content (correct)
- Purity
What is a key step in the isolation of yeast RNA involving heating with NaOH?
What is a key step in the isolation of yeast RNA involving heating with NaOH?
- Precipitating RNA with alcohol
- Inactivating RNA-degrading enzymes (correct)
- Filtering lipids
- Extracting water-soluble proteins
What is the role of glacial acetic acid during the experiment procedure?
What is the role of glacial acetic acid during the experiment procedure?
Why is the purine N-glycosyl bond in nucleic acids susceptible to acid hydrolysis?
Why is the purine N-glycosyl bond in nucleic acids susceptible to acid hydrolysis?
What is the outcome of repeated washing with alcohol and ether during the extraction process?
What is the outcome of repeated washing with alcohol and ether during the extraction process?
What are the two major types of nucleic acids?
What are the two major types of nucleic acids?
What is the ratio that indicates tissues with high RNA content?
What is the ratio that indicates tissues with high RNA content?
What is the solubility of nucleic acids in alcohol?
What is the solubility of nucleic acids in alcohol?
Which type of hydrolysis yields nucleotides and nucleosides?
Which type of hydrolysis yields nucleotides and nucleosides?
What does complete acid hydrolysis of nucleic acids yield?
What does complete acid hydrolysis of nucleic acids yield?
Which tissues are good sources of DNA based on their nuclear/cytoplasmic ratio?
Which tissues are good sources of DNA based on their nuclear/cytoplasmic ratio?
Which of the following accurately describes the solubility of RNA in various solvents?
Which of the following accurately describes the solubility of RNA in various solvents?
What is the expected outcome when concentrated hydrochloric acid (HCl) is added to an RNA solution in dilute alkali?
What is the expected outcome when concentrated hydrochloric acid (HCl) is added to an RNA solution in dilute alkali?
What is the purpose of the biuret test in the context of RNA analysis?
What is the purpose of the biuret test in the context of RNA analysis?
Which of the following statements accurately describes the behavior of RNA upon boiling and subsequent cooling?
Which of the following statements accurately describes the behavior of RNA upon boiling and subsequent cooling?
Which of the following tests can be used to determine the presence of purine bases in RNA?
Which of the following tests can be used to determine the presence of purine bases in RNA?
What is the expected outcome when RNA undergoes hydrolysis?
What is the expected outcome when RNA undergoes hydrolysis?
