Biochemistry Lab Equipment Quiz 2nd Year
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Questions and Answers

Which of the following is NOT a type of laboratory glassware?

  • Graduated cylinder
  • Beaker
  • Fork (correct)
  • Test tube
  • What does pH measure?

  • The volume of a liquid
  • The weight of a solid
  • The temperature of a solution
  • The hydrogen ion concentration (correct)
  • A solution with a pH less than 7 is considered neutral.

    False

    What is the formula for calculating pH?

    <p>pH = -log[H+]</p> Signup and view all the answers

    What is the range of pH values?

    <p>0 to 14</p> Signup and view all the answers

    Who proposed the Bronsted-Lowry theory?

    <p>Johannes Bronsted and Thomas Lowry</p> Signup and view all the answers

    Acids are substances that can donate a ______ ion.

    <p>hydrogen</p> Signup and view all the answers

    Bases are substances that accept ______ ions.

    <p>hydrogen</p> Signup and view all the answers

    Define potential of hydroxide (pOH).

    <p>pOH measures the hydroxide ion concentration.</p> Signup and view all the answers

    Study Notes

    Laboratory Glasswares

    • Essential for performing biochemical analyses with specific functions in the laboratory.
    • Key glassware types include graduated cylinders, beakers, Erlenmeyer flasks, test tubes, crucibles, and more.
    • Devices like spectrophotometers, analytical balances, and micropipettes aid in experiments.
    • Common lab functions: measuring solutions, holding reagents, facilitating chemical reactions, and heating.

    pH and Its Importance

    • pH measures the acidity or basicity of a solution, with a scale ranging from 0 to 14.
    • Acidic solutions have pH < 7, neutral solutions have pH = 7, and basic solutions have pH > 7.
    • Hydrogen ion (H+) concentration directly affects biochemical reactions in living organisms.

    Definition of pOH

    • pOH measures hydroxide ion (OH-) concentration and is represented as the negative logarithm of [OH-].
    • The relationship between pH and pOH allows for a comprehensive understanding of solution properties.

    Definitions of Acids and Bases

    • Svante Arrhenius: Acids produce H+ ions in an aqueous solution; bases produce OH- ions.
    • Gilbert N. Lewis: Acids are electron-pair acceptors, while bases are electron-pair donors.
    • Bronsted-Lowry Theory: A more widely accepted definition where acids are proton donors and bases are proton acceptors.

    Reaction Between HCl and Water

    • Not explicitly detailed, but implies that the reaction demonstrates the transfer of protons, relevant to understanding acidity.

    Role of Buffers

    • Buffers help maintain stable pH levels in biological systems, supporting homeostasis in organisms.

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    Description

    Test your knowledge on laboratory glassware and apparatus used in biochemistry. This quiz covers essential tools required for performing biochemical analyses effectively. Perfect for 2nd-year BSMT students preparing for their midterms.

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