Biochemistry Lab Equipment Quiz 2nd Year
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Questions and Answers

Which of the following is NOT a type of laboratory glassware?

  • Graduated cylinder
  • Beaker
  • Fork (correct)
  • Test tube

What does pH measure?

  • The volume of a liquid
  • The weight of a solid
  • The temperature of a solution
  • The hydrogen ion concentration (correct)

A solution with a pH less than 7 is considered neutral.

False (B)

What is the formula for calculating pH?

<p>pH = -log[H+]</p> Signup and view all the answers

What is the range of pH values?

<p>0 to 14 (B)</p> Signup and view all the answers

Who proposed the Bronsted-Lowry theory?

<p>Johannes Bronsted and Thomas Lowry (D)</p> Signup and view all the answers

Acids are substances that can donate a ______ ion.

<p>hydrogen</p> Signup and view all the answers

Bases are substances that accept ______ ions.

<p>hydrogen</p> Signup and view all the answers

Define potential of hydroxide (pOH).

<p>pOH measures the hydroxide ion concentration.</p> Signup and view all the answers

Study Notes

Laboratory Glasswares

  • Essential for performing biochemical analyses with specific functions in the laboratory.
  • Key glassware types include graduated cylinders, beakers, Erlenmeyer flasks, test tubes, crucibles, and more.
  • Devices like spectrophotometers, analytical balances, and micropipettes aid in experiments.
  • Common lab functions: measuring solutions, holding reagents, facilitating chemical reactions, and heating.

pH and Its Importance

  • pH measures the acidity or basicity of a solution, with a scale ranging from 0 to 14.
  • Acidic solutions have pH < 7, neutral solutions have pH = 7, and basic solutions have pH > 7.
  • Hydrogen ion (H+) concentration directly affects biochemical reactions in living organisms.

Definition of pOH

  • pOH measures hydroxide ion (OH-) concentration and is represented as the negative logarithm of [OH-].
  • The relationship between pH and pOH allows for a comprehensive understanding of solution properties.

Definitions of Acids and Bases

  • Svante Arrhenius: Acids produce H+ ions in an aqueous solution; bases produce OH- ions.
  • Gilbert N. Lewis: Acids are electron-pair acceptors, while bases are electron-pair donors.
  • Bronsted-Lowry Theory: A more widely accepted definition where acids are proton donors and bases are proton acceptors.

Reaction Between HCl and Water

  • Not explicitly detailed, but implies that the reaction demonstrates the transfer of protons, relevant to understanding acidity.

Role of Buffers

  • Buffers help maintain stable pH levels in biological systems, supporting homeostasis in organisms.

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Description

Test your knowledge on laboratory glassware and apparatus used in biochemistry. This quiz covers essential tools required for performing biochemical analyses effectively. Perfect for 2nd-year BSMT students preparing for their midterms.

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