Optical Scattering and Spectrophotometry Quiz
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Questions and Answers

What is the primary factor that dictates the scattering of light in optical scattering?

The physical properties of the molecules and their interactions with light.

Define the absorption coefficient in the context of a photon interacting with matter.

The absorption coefficient quantifies how much light is absorbed per unit distance in a medium.

Explain the significance of the refractive index in optical scattering.

The refractive index measures how much light slows down and bends when entering a medium.

What role does the absorption cross-section play in photon interactions?

<p>The absorption cross-section indicates the probability of a photon being absorbed by a target molecule.</p> Signup and view all the answers

In the context of molecular scattering, what does the scattering coefficient represent?

<p>The scattering coefficient measures the amount of light scattered per unit distance in a medium.</p> Signup and view all the answers

What is the purpose of spectrophotometry?

<p>To measure the absorption spectra of substances.</p> Signup and view all the answers

What formula is used to calculate the percentage of $SO_2$?

<p>$SO_2$ (%) = \frac{C_{H_2O_2}}{C_{H_2O_2} + C_{Hb}}</p> Signup and view all the answers

Write the equation relating detected intensity to absorption.

<p>I_L(\lambda) = I_0(\lambda) exp(-μ_a(\lambda)d)</p> Signup and view all the answers

What does the absorption coefficient $μ_a(\lambda)$ signify?

<p>It measures how much light is absorbed by the substance at wavelength $\lambda$.</p> Signup and view all the answers

How can you calculate the absorption coefficient from detected and incident intensity?

<p>$μ_a(\lambda) = -\frac{1}{d}ln(\frac{I_L(\lambda)}{I_0(\lambda)})$</p> Signup and view all the answers

Study Notes

Spectroscopy

  • Spectroscopy is a technique used to measure the intensity of light as it passes through a sample.
  • The detected intensity can be written as:
  • IL(λ) = Io(λ) exp(-μa(λ)d)
  • μa(λ) = -1/d ln(IL(λ)/Io(λ))

Beer's Law

  • Beer's law describes the attenuation of light as it passes through a medium.

  • The intensity of the light will decrease in the direction of propagation as the light is absorbed.

  • The formula for Beer's law is:

  • φ = φ0 exp(-μaz)

  • where φ is the light intensity after passing through the medium, φ0 is the initial light intensity, μa is the absorption coefficient, and z is the distance travelled.

  • A plane wave of continuous light irradiating a purely absorbing, non-scattering medium.

Spectrophotometry

  • Spectrophotometry is a technique used to determine the concentration of a substance in a solution by measuring the amount of light absorbed or transmitted by the solution at different wavelengths.
  • This can be described as:
  • s02 (%) = CH2O2/ (CH2O2 + CHb).
  • Given a sample that contains several known chromophores, it is often possible to estimate the concentrations of each constituent by making measurements at multiple wavelengths.

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Description

Test your knowledge on optical scattering principles and spectrophotometry. This quiz covers various aspects such as the absorption coefficient, refractive index, and formulas for calculating absorption and intensity. Perfect for students studying physics or optics!

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