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Molecular Biology: Cohesin-Mediated Gene Regulation

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Molecular Biology: Cohesin-Mediated Gene Regulation

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Questions and Answers

What is the primary function of Cohesin in the context of gene regulation?

To facilitate sister chromatid separation during mitosis

To mediate chromatin looping at multiple gene loci (correct)

To regulate DNA replication during S-phase

To catalyze histone modification reactions

What is the term for self-interacting genomic regions that physically interact with each other more frequently than with sequences outside the region?

Histone modification domains

Topological associating domains (TADs) (correct)

Nucleosome organization territories

Chromosomal loops

How many histone proteins are typically found in a histone octamer?

8 (correct)

What is the name of the technique used to assess chromosomal looping and topologically associated domains?

HiC Signup and view all the answers

What is the typical length of DNA wrapped around a histone octamer in a nucleosome?

146 bases Signup and view all the answers

What is the primary function of CTCF in the context of chromatin organization?

To mark physical boundary distinctions Signup and view all the answers

What is the primary function of euchromatin?

To make genes accessible for transcription Signup and view all the answers

What is the term for the complex folded surfaces of chromosomal territories?

Chromosomal territories Signup and view all the answers

What is the purpose of regulatory sequences in eukaryotic gene structure?

To act as molecular switches Signup and view all the answers

What is the term for the highly compacted, repressive gene state?

Heterochromatin Signup and view all the answers

What is the term for the 3D organization of chromatin in the nucleus?

Chromosomal territories Signup and view all the answers

What is the term for the breaking and rejoining of chromatin fibers?

Chromatin remodeling Signup and view all the answers

What is the primary function of Histone 1 (H1)?

To link adjacent nucleosome core particles Signup and view all the answers

Which of the following families of chromatin remodeling enzymes is involved in spatially and temporally regulating gene expression?

CHD Signup and view all the answers

What is the characteristic of the 5’ and 3’ ends of genes?

They are regions without nucleosomes Signup and view all the answers

What is the result of mutations in almost all 9 CHD proteins?

Various pathologies Signup and view all the answers

What is the characteristic of chromatin remodeling enzymes?

They are ATP-dependent Signup and view all the answers

What is the function of chromatin remodeling enzymes in transcription regulation?

To positively regulate the formation of nucleosome free regions Signup and view all the answers