60 Questions
Match the following types of media with their descriptions:
Broth = Liquid media used for the growth of microorganisms Solid = Media containing agar, used for the growth of microorganisms Plate = Solid media in a petri dish used for culturing bacteria Slant = Solid media in a test tube with a slanted surface for culturing bacteria
Match the following methods of inoculating media with their descriptions:
Streak plate method = Method used to obtain isolated, individual colonies on agar plate Dip-n-Swish Method = Method used for inoculating broth media Fish Tail method = Method used for inoculating agar slant Straight Line Stab = Method used for inoculating agar deep
Match the following components of media with their descriptions:
Nutrient = General purpose media for the growth of microorganisms Selective and Differential Media = Media components with specific properties to select for or differentiate between microorganisms Agar = Solidifying agent used in solid media Inoculating loop = Tool used for streaking and inoculating media
Match the following terms with their meanings:
Inoculate = The process by which microorganisms are added to media Colony = A group of genetically identical bacteria Agar Plate = Solid media used for streaking to isolate individual colonies Bacterium = Singular form of bacteria
Match the following bacterial culturing methods with their descriptions:
Streak to isolate/Quadrant Method = Method used to obtain isolated, individual colonies on agar plate Dip-n-Swish Method = Method used for inoculating broth media Fish Tail method = Method used for inoculating agar slant Straight Line Stab = Method used for inoculating agar deep
Match the following methods with their usage:
Dip-n-Swish Method = Used to inoculate broth Fish Tail Method = Used to inoculate an agar slant Straight Line Stab = Used to inoculate an agar deep Durham tube = Used to determine carbohydrate fermentation with or without gas production
Match the following culture media with their characteristics:
MSA = For isolation and differentiation of Staphylococcus species Starch Agar = To check for the presence of amylase via starch hydrolysis EMB = Isolate Gram negative bacteria Phenol Red Broths = Used to determine carbohydrate fermentation with or without gas production
Match the following agar types with their usage:
MacConkey Agar = Used to isolate and differentiate gram negative organisms Blood agar = Differential based on hemolysis, isolation and cultivation of fastidious bacteria Eosin Methylene Blue Agar = Isolate Gram neg. bacteria Carbohydrate utilization tubes = Used as differential media
Match the following hemolysis types with their characteristics:
Beta-hemolysis = Complete breakdown of hemoglobin; clearing of medium Alpha-hemolysis = Incomplete breakdown of hemoglobin No hemolysis (gamma-hemolysis) = No hemolysins present; no change in medium Hemolysis = Differential based on hemolysis, isolation and cultivation of fastidious bacteria
Match the following tube types with their characteristics:
SIM tubes = Sulfide, indole, motility Phenol Red Broths = Used to determine carbohydrate fermentation with or without gas production Carbohydrate utilization tubes = Used as differential media Starch Agar = To check for the presence of amylase via starch hydrolysis
Match the following bacterial inoculation methods with their respective media:
Dip-n-Swish Method = Broth Fish Tail Method = Agar slant Straight Line Stab = Agar deep Durham tube = Carbohydrate fermentation determination
Match the following culture media with their purposes:
MSA = Isolation and differentiation of Staphylococcus species Starch Agar = Presence of amylase via starch hydrolysis EMB = Isolation of Gram negative bacteria Phenol Red Broths = Carbohydrate fermentation with or without gas production
Match the following agar types with their respective bacteria isolation/differentiation:
MacConkey Agar = Gram negative organisms Blood agar = Fastidious bacteria Eosin Methylene Blue Agar = Gram negative bacteria Carbohydrate utilization tubes = Differential media
Match the following hemolysis types with their characteristics:
Beta-hemolysis = Complete breakdown of hemoglobin Alpha-hemolysis = Incomplete breakdown of hemoglobin No hemolysis (gamma-hemolysis) = No change in medium Hemolysis = Isolation and cultivation of fastidious bacteria
Match the following tube types with their purposes:
SIM tubes = Sulfide, indole, motility Phenol Red Broths = Carbohydrate fermentation determination Carbohydrate utilization tubes = Differential media Starch Agar = Presence of amylase via starch hydrolysis
Which type of media contains an ingredient that encourages the growth of some bacteria, but discourages the growth of other bacteria?
Selective media
What is the purpose of using MacConkey agar?
Isolate Gram-negative bacteria based on lactose fermentation
Which agar is used for the isolation and differentiation of Staphylococcus species?
MSA agar
What does the presence of black color in SIM tubes indicate?
Presence of hydrogen sulfide (H2S)
What is the purpose of using Phenol Red Broths?
Determine carbohydrate fermentation with or without gas production
Which type of media helps separate bacteria based on a metabolic process such as enzyme tests and carbohydrates?
Differential media
What does the presence of hot pink colonies indicate on MacConkey agar?
Lactose fermentation
What does the absence of hemolysis indicate in blood agar?
Gamma-hemolysis
What is the purpose of using EMB agar?
Isolate Gram-negative bacteria
What is the indicator for carbohydrate fermentation in Phenol Red Broths?
Phenol red
What is the purpose of the streak plate method on an agar plate?
To obtain isolated, individual colonies
Which type of media is a solid media composed of broth and agar?
Slant
What is the purpose of the dip-n-swish method on a broth media?
To ensure even distribution of bacteria in the broth
What is the main difference between nutrient media and selective media components?
Nutrient media promotes the growth of specific bacteria, while selective media inhibits the growth of unwanted bacteria
What is the process of adding microorganisms to media called?
Inoculate
Which method is used for inoculating agar slants?
Fish Tail method
What type of media is used to obtain isolated, individual colonies?
Solid media
What is the purpose of the straight line stab on agar deep?
To allow anaerobic bacteria to grow along the line of stab
What is the primary difference between plate and slant as types of solid media?
The shape of the container they are held in
What is the main function of agar in solid media?
To solidify the media and provide a platform for bacterial growth
What are the six types of general culture media mentioned in the text?
The six types of general culture media mentioned are defined media, complex media, selective media, differential media, anaerobic media, and transport media.
What is the purpose of using MacConkey agar?
MacConkey agar is used to isolate and differentiate gram-negative organisms based on lactose fermentation and contains bile salts and crystal violet as selective agents.
What are the three reasons for inoculating on a differential and selective media?
The three reasons for inoculating on a differential and selective media are to culture all bacteria present and see which, if any, predominates; to differentiate species by certain characteristic responses to media ingredients; and to selectively encourage the growth of species of interest while suppressing normal flora.
What is the purpose of Phenol Red Broths?
Phenol Red Broths are used to determine carbohydrate fermentation with or without gas production.
What are the characteristics of Eosin Methylene Blue Agar (EMB) colonies indicating lactose fermentation?
Metallic green/blue-black colonies indicate vigorous lactose fermenters with an acidic environment; dark purple colonies indicate slower fermenters with an acidic environment; light pink or colorless colonies indicate no fermentation of lactose.
Define solid media and provide an example.
Solid media is a type of culture media that contains agar to solidify it. An example of solid media is agar plates.
What is the purpose of the streak plate method on an agar plate?
The purpose of the streak plate method on an agar plate is to obtain isolated, individual colonies of microorganisms.
Explain the process of inoculating agar slants.
The process of inoculating agar slants involves using the fish tail method to streak the surface of the slant with the inoculating loop.
What is the purpose of using the dip-n-swish method on a broth media?
The purpose of using the dip-n-swish method on a broth media is to disperse the bacteria throughout the liquid medium.
What are the types of solid media mentioned in the text, and how do they differ?
The types of solid media mentioned are plates, slants, and deep. They differ in their physical form and the surface area available for bacterial growth.
What is the purpose of the streak plate method on an agar plate?
To obtain isolated, individual colonies
What is the primary difference between nutrient media and selective media components?
Selective media components encourage the growth of specific bacteria, while nutrient media support the growth of a wide range of microorganisms
What is the indicator for carbohydrate fermentation in Phenol Red Broths?
Yellow color
What does the presence of hot pink colonies indicate on MacConkey agar?
Lactose fermentation
What is the process of adding microorganisms to media called?
Inoculation
Which type of media is used to obtain isolated, individual colonies?
Solid media
What are the two types of solid media mentioned in the text, and how do they differ?
Plate and Slant; Plate media is used for general purposes while Slant media is selective
What type of media is Mannitol Salt Agar (MSA)?
Differential and selective media
What is the purpose of Eosin Methylene Blue Agar (EMB)?
Isolate Gram-negative bacteria
What is the indicator for carbohydrate fermentation in Phenol Red Broths?
Phenol red
What does the presence of hot pink colonies indicate on MacConkey Agar?
Lactose fermentation
What is the primary purpose of using blood agar?
Differential based on hemolysis, isolation, and cultivation of fastidious bacteria
What does the absence of hemolysis indicate in blood agar?
No hemolysis (gamma-hemolysis)
What is the purpose of using MacConkey Agar?
Isolate and differentiate Gram-negative organisms based on lactose fermentation
What is the purpose of the Fish Tail Method?
Inoculate an Agar Slant
Test your knowledge of media terms used in microbiology with this quiz. Explore different types of media, components, and the process of inoculation. Sharpen your understanding of microbiology terminology essential for laboratory work.
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