Microbiology Lab: Colony Characteristics and Aseptic Technique

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What is the origin of a bacterial colony?

A single bacterium or a few bacteria (correct)

Multiple bacteria from different sources

A mix of bacteria from the environment

A contaminated growth medium

What is the purpose of inoculation in a microbiology laboratory?

To observe colony characteristics

To prevent contamination of growth media

To introduce bacteria into a sterile growth medium (correct)

To sterilize the work area

Why is it important to minimize the time that cultures and growth media are open to the environment?

To observe colony characteristics

To promote bacterial growth

To disinfect the work area

To prevent contamination from the environment (correct)

What is the definition of a sterile material?

A material that has no living organisms present Signup and view all the answers

What is the main reason for using aseptic techniques in a microbiology laboratory?

To prevent the transfer of bacteria from the surrounding environment into a culture medium Signup and view all the answers

What is a consequence of not following aseptic techniques in a microbiology laboratory?

Contamination of growth media Signup and view all the answers

What is the purpose of heat fixing the bacterial specimen on the slide?

To kill the cells and adhere them to the slide Signup and view all the answers

What is the main function of growth media in the laboratory?

To sustain the growth of microorganisms Signup and view all the answers

What is the purpose of using an inoculation loop or needle in the preparation of bacterial specimens?

To pick up a small amount of bacteria Signup and view all the answers

What is the consequence of not heat fixing the bacterial specimen properly?

The bacteria will be rinsed off the slide Signup and view all the answers

What is the difference between liquid and solid growth media?

Solid media contains agar, while liquid media does not Signup and view all the answers

Why should the slide be air-dried before heat fixing the bacterial specimen?

To remove excess moisture from the slide Signup and view all the answers

What is the purpose of adding carbol fuchsin in the Koch Kinyoun Staining Procedure?

To differentiate between acid-fast and non-acid-fast organisms Signup and view all the answers

Why is it important to break up clumps of M. smegmatis before staining?

To ensure even staining of the bacteria Signup and view all the answers

What is the function of Acid-alcohol in the Koch Kinyoun Staining Procedure?

To decolorize the primary stain in non-acid-fast organisms Signup and view all the answers

What is the purpose of adding methylene blue in the Koch Kinyoun Staining Procedure?

To act as a counterstain for non-acid-fast organisms Signup and view all the answers

Why are endospores difficult to eliminate from the environment?

Because they are highly resistant to radiation, desiccation, and disinfectants Signup and view all the answers

What is the purpose of preparing a smear with a mixture of M. smegmatis and Micrococcus luteus?

To differentiate between acid-fast and non-acid-fast organisms Signup and view all the answers

Microbiology Lab: Colony Characteristics and Aseptic Technique

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