18 Questions
What is the origin of a bacterial colony?
A single bacterium or a few bacteria
What is the purpose of inoculation in a microbiology laboratory?
To introduce bacteria into a sterile growth medium
Why is it important to minimize the time that cultures and growth media are open to the environment?
To prevent contamination from the environment
What is the definition of a sterile material?
A material that has no living organisms present
What is the main reason for using aseptic techniques in a microbiology laboratory?
To prevent the transfer of bacteria from the surrounding environment into a culture medium
What is a consequence of not following aseptic techniques in a microbiology laboratory?
Contamination of growth media
What is the purpose of heat fixing the bacterial specimen on the slide?
To kill the cells and adhere them to the slide
What is the main function of growth media in the laboratory?
To sustain the growth of microorganisms
What is the purpose of using an inoculation loop or needle in the preparation of bacterial specimens?
To pick up a small amount of bacteria
What is the consequence of not heat fixing the bacterial specimen properly?
The bacteria will be rinsed off the slide
What is the difference between liquid and solid growth media?
Solid media contains agar, while liquid media does not
Why should the slide be air-dried before heat fixing the bacterial specimen?
To remove excess moisture from the slide
What is the purpose of adding carbol fuchsin in the Koch Kinyoun Staining Procedure?
To differentiate between acid-fast and non-acid-fast organisms
Why is it important to break up clumps of M. smegmatis before staining?
To ensure even staining of the bacteria
What is the function of Acid-alcohol in the Koch Kinyoun Staining Procedure?
To decolorize the primary stain in non-acid-fast organisms
What is the purpose of adding methylene blue in the Koch Kinyoun Staining Procedure?
To act as a counterstain for non-acid-fast organisms
Why are endospores difficult to eliminate from the environment?
Because they are highly resistant to radiation, desiccation, and disinfectants
What is the purpose of preparing a smear with a mixture of M. smegmatis and Micrococcus luteus?
To differentiate between acid-fast and non-acid-fast organisms
In this lab, you will learn about the characteristics of bacterial colonies, including their forms, margins, elevations, and colors. You will also understand the importance of aseptic technique and inoculation in microbiology. Test your knowledge on identifying unknown bacteria and more!
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