Microbiology Incubation and Bacterial Population Counts Quiz
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Questions and Answers

What does the y-axis represent in a graph plotting the logarithmic number of viable organisms against incubation time?

  • Incubation time
  • Number of living cells
  • Logarithmic number of viable organisms (correct)
  • Type of culture media
  • Which organisms can survive and multiply only within living host cells?

  • Viruses (correct)
  • Protozoa
  • Fungi
  • Bacteria
  • Which laboratory method is used to culture some obligate intracellular pathogens like rickettsias and chlamydias?

  • Growing in cell cultures (correct)
  • Incubating in spectrophotometers
  • Inoculating into brain heart infusion (BHI)
  • Growing on Sabouraud dextrose agar
  • Which solid culture medium is selective for fungi due to its low pH?

    <p>Sabouraud dextrose agar (SDA)</p> Signup and view all the answers

    Why is caution necessary when culturing fungi in the laboratory?

    <p>Some are highly infectious</p> Signup and view all the answers

    Which method is most commonly used to culture protozoa in microbiology laboratories?

    <p>Culturing in cell cultures</p> Signup and view all the answers

    What is the purpose of using a spectrophotometer in microbiology?

    <p>To measure turbidity of the medium as an indicator of growth</p> Signup and view all the answers

    How does viable plate count determine the number of viable bacteria in a liquid sample?

    <p>By making serial dilutions and inoculating onto nutrient agar</p> Signup and view all the answers

    In microbiology, what is the purpose of a CO2 incubator?

    <p>To maintain the appropriate atmosphere for bacterial growth</p> Signup and view all the answers

    What technique is used to determine bacterial contamination of drinking water?

    <p>Viable plate count</p> Signup and view all the answers

    How is a population growth curve typically determined in microbiology?

    <p>By collecting samples at intervals to determine viable organisms</p> Signup and view all the answers

    Which method is used to determine either the total number of bacterial cells or the number of viable cells in a liquid sample?

    <p>Viable plate count</p> Signup and view all the answers

    Which type of medium contains special nutrients that promote the growth of fastidious organisms?

    <p>Enriched medium</p> Signup and view all the answers

    Which medium inhibits the growth of gram-positive bacteria and promotes the growth of gram-negative bacteria?

    <p>MacConkey agar</p> Signup and view all the answers

    What type of agar inhibits the growth of gram-negative bacteria and promotes the growth of gram-positive bacteria?

    <p>Phenylethyl alcohol agar</p> Signup and view all the answers

    Which medium permits differentiation of organisms that grow on it?

    <p>Differential medium</p> Signup and view all the answers

    What is added to selective media to discourage the growth of certain organisms while allowing the growth of desired organisms?

    <p>Inhibitors</p> Signup and view all the answers

    Why is aseptic technique practiced in microbiology labs?

    <p>To exclude microbes from specific areas</p> Signup and view all the answers

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