Metabolic Pathways and Energy Production
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Questions and Answers

Which statement accurately describes the relationship between catabolism and ATP?

  • Catabolism consumes ATP to build complex molecules from simpler ones.
  • Catabolism and ATP production are unrelated processes within a cell.
  • Catabolism transports ATP out of the cell for use in other organisms.
  • Catabolism produces ATP by breaking down complex molecules. (correct)

During periods of high energy demand, which process would be prioritized by the cell to maintain energy balance?

  • The synthesis of glycogen from glucose molecules.
  • The synthesis of fatty acids from acetyl-CoA.
  • The breakdown of stored fats and glycogen. (correct)
  • The breakdown of ATP into ADP and inorganic phosphate.

In which cellular location does beta-oxidation primarily occur?

  • Cytoplasm
  • Mitochondria (correct)
  • Endoplasmic Reticulum
  • Golgi Apparatus

Which of the following is the primary end product of glycolysis that then enters the Citric Acid Cycle under aerobic conditions?

<p>Acetyl-CoA (D)</p> Signup and view all the answers

Which of the following is NOT directly produced during the Citric Acid Cycle?

<p>Pyruvate (B)</p> Signup and view all the answers

What is the main function of oxidative phosphorylation in cellular respiration?

<p>To generate a large amount of ATP using the electron transport chain and chemiosmosis. (A)</p> Signup and view all the answers

Which of the following molecules acts as a crucial link between glycolysis and the citric acid cycle?

<p>Acetyl-CoA (D)</p> Signup and view all the answers

If a cell has a surplus of ATP and does not require immediate energy, which of the following pathways is most likely to be downregulated?

<p>All of the above (D)</p> Signup and view all the answers

Which of the following is NOT a primary function of the viral capsid?

<p>Replicating the viral genome within the host cell. (B)</p> Signup and view all the answers

A researcher is studying a newly discovered virus. Initial observations show a protein shell enclosing the genetic material. What is the correct term for this protein shell?

<p>Capsid. (A)</p> Signup and view all the answers

Which cellular process would be MOST affected by a drug that inhibits microtubule depolymerization?

<p>Formation of the mitotic spindle during cell division. (A)</p> Signup and view all the answers

How do motor proteins, such as kinesin and dynein, utilize microtubules to transport cargo within a cell?

<p>By using ATP to move along microtubules. (C)</p> Signup and view all the answers

During which phase of the cell cycle do microtubules play the MOST significant role in chromosome separation?

<p>Metaphase. (D)</p> Signup and view all the answers

Why is the dynamic instability of microtubules essential for cellular functions such as cell division and intracellular transport?

<p>It allows microtubules to quickly assemble and disassemble, providing flexibility and control. (A)</p> Signup and view all the answers

A researcher observes that the energy input into a cellular process is equal to the energy output plus the heat generated. Which law of thermodynamics is exemplified in this observation?

<p>First Law of Thermodynamics. (B)</p> Signup and view all the answers

If a drug prevents the hydrolysis of GTP bound to tubulin dimers, what direct effect would you expect to see in microtubule behavior?

<p>Microtubules would become more stable and less dynamic. (D)</p> Signup and view all the answers

Which of the following processes is NOT a primary function of anabolism in cells?

<p>Breaking down complex molecules to release energy. (C)</p> Signup and view all the answers

How do cells primarily regulate anabolic pathways to ensure energy balance?

<p>Via feedback inhibition, where the end-products of anabolic pathways can inhibit their own synthesis. (C)</p> Signup and view all the answers

What is the primary role of catabolism in cellular metabolism?

<p>To release energy by breaking down complex molecules. (D)</p> Signup and view all the answers

Which characteristic defines catabolic reactions regarding energy?

<p>Exergonic, releasing energy as they break down molecules. (D)</p> Signup and view all the answers

Which set of molecules is most commonly involved in catabolic reactions?

<p>Carbohydrates, lipids, and proteins. (A)</p> Signup and view all the answers

What is the primary significance of synthesizing energy-rich molecules like glycogen during anabolism?

<p>They serve as stored energy that can be accessed when needed. (D)</p> Signup and view all the answers

How does insulin influence anabolic pathways in the body?

<p>It promotes glycogen synthesis and protein synthesis. (C)</p> Signup and view all the answers

What is the importance of maintaining a balance between anabolism and catabolism in cells?

<p>It ensures that energy expenditure and energy synthesis are matched for efficient function. (C)</p> Signup and view all the answers

In the context of thermodynamics, what does a negative value of Gibbs free energy (G) indicate for a reaction?

<p>The reaction will occur spontaneously. (A)</p> Signup and view all the answers

Which of the following best describes the relationship between enthalpy (H), entropy (S), and Gibbs free energy (G) at a constant temperature (T)?

<p>$G = H - TS$ (A)</p> Signup and view all the answers

How do hormones regulate catabolic activity in the body?

<p>They can inhibit fat breakdown when energy is sufficient or promote catabolism during low-energy states. (A)</p> Signup and view all the answers

What is the significance of temperature (T) in the equation $G = H - TS$ in the context of predicting reaction spontaneity?

<p>Temperature influences the entropy (S) contribution to Gibbs free energy (G). (C)</p> Signup and view all the answers

What is the primary role of feedback mechanisms in metabolic pathways?

<p>To maintain homeostasis by stimulating or inhibiting metabolic activities based on metabolite concentration and energy availability. (A)</p> Signup and view all the answers

Why do cells prioritize catabolism of stored molecules during periods of scarcity?

<p>To rapidly mobilize energy reserves, ensuring essential cellular functions continue under low-energy conditions. (A)</p> Signup and view all the answers

How does the breakdown of glucose contribute to ATP formation in terms of Gibbs free energy (G)?

<p>Glucose breakdown leads to a negative G, and the released energy is used to form ATP. (A)</p> Signup and view all the answers

If a certain biological reaction has a positive change in enthalpy (H) and a decrease in entropy (S), what condition would most likely make the reaction spontaneous?

<p>Decreasing the temperature significantly. (B)</p> Signup and view all the answers

In the context of metabolism, what is the role of enzymes?

<p>To catalyze each step in the chemical reactions of metabolic pathways, facilitating efficient reactions. (A)</p> Signup and view all the answers

In thermodynamic terms, what does 'S' signify, and how does its value relate to the state of a system?

<p>'S' signifies entropy, indicating disorder; higher values mean greater disorder. (C)</p> Signup and view all the answers

How does photosynthesis contribute to the energy needs of most life forms?

<p>It serves as the process by which organisms use sunlight to construct energy-rich biological molecules. (D)</p> Signup and view all the answers

ATP hydrolysis provides energy for biosynthetic reactions. How does ATP hydrolysis influence Gibbs Free Energy (G) in building molecules?

<p>ATP hydrolysis results in a positive G; this energy drives molecule building. (A)</p> Signup and view all the answers

Which of the following best describes the relationship between catabolism and anabolism?

<p>Catabolism breaks down complex molecules to release energy, while anabolism builds complex molecules using energy. (C)</p> Signup and view all the answers

In the equation $G = H - TS$, what does a negative value of G indicate about a reaction?

<p>The reaction releases energy and is spontaneous, or thermodynamically favorable. (D)</p> Signup and view all the answers

If a researcher wants to study a reaction at normal human body temperature, what Kelvin value should they use for 'T' in their thermodynamic calculations?

<p>310 K (A)</p> Signup and view all the answers

What is the significance of the change in free energy (G) in a reaction?

<p>It indicates the amount of usable energy available to do cellular work. (B)</p> Signup and view all the answers

In biological systems, what is the implication of a reaction having a negative Gibbs free energy (G)?

<p>The reaction occurs spontaneously, releasing free energy that can be used to do work. (B)</p> Signup and view all the answers

A researcher observes a metabolic reaction with a positive Gibbs free energy. What can they infer about this reaction under standard conditions?

<p>The reaction requires an input of energy to proceed. (C)</p> Signup and view all the answers

How does an increase in entropy (S) affect the energy available for cellular processes within a closed system?

<p>It decreases the amount of usable energy, reflecting energy transformation inefficiencies. (C)</p> Signup and view all the answers

What is the primary implication of the second law of thermodynamics for biological systems?

<p>The entropy of a closed system tends to increase over time. (A)</p> Signup and view all the answers

During the hydrolysis of ATP, what is the immediate fate of the energy that is released?

<p>It is harnessed to perform cellular work. (C)</p> Signup and view all the answers

What is the state of Gibbs free energy (G) in a system that has reached thermodynamic equilibrium?

<p>Gibbs free energy is minimized, and there is no net change in the system. (B)</p> Signup and view all the answers

A cell biologist is studying a new metabolic pathway. They observe that ATP is being consumed at a high rate. What process is most likely occurring in the cell?

<p>The cell is performing energy-requiring processes such as biosynthesis or active transport. (A)</p> Signup and view all the answers

During the formation of complex biomolecules such as proteins, what role does enthalpy (H) play, and how is it influenced by ATP?

<p>The reaction requires energy input, increasing enthalpy, which can be supplied by ATP hydrolysis. (D)</p> Signup and view all the answers

Which of the following factors directly influence the Gibbs free energy (∆G) of a reaction, as described by the equation ∆G = ∆H – T∆S?

<p>The change in enthalpy (∆H) and the change in entropy (∆S) at a constant temperature (T). (B)</p> Signup and view all the answers

How do feedback mechanisms primarily contribute to maintaining metabolic homeostasis within a cell?

<p>By inhibiting or activating enzymes in metabolic pathways in response to changing metabolic conditions. (B)</p> Signup and view all the answers

Consider a reaction at equilibrium. How would increasing the temperature affect the Gibbs free energy (G) if the reaction involves an increase in entropy (S)?

<p>Decrease G, making the reaction more spontaneous. (D)</p> Signup and view all the answers

Which of the following accurately describes how enzymes influence metabolic reactions?

<p>Enzymes lower the activation energy of a reaction, thereby increasing the reaction rate. (C)</p> Signup and view all the answers

In the context of cellular metabolism, what determines whether a reaction will occur spontaneously?

<p>Whether the change in Gibbs free energy (∆G) is negative. (C)</p> Signup and view all the answers

How do cells primarily utilize the energy released during catabolic reactions?

<p>To synthesize ATP and other energy-rich molecules that power anabolic reactions. (D)</p> Signup and view all the answers

During periods of starvation, the body relies on catabolic processes to maintain energy levels. Which hormonal response is most likely to promote these catabolic processes?

<p>Increased glucagon secretion to stimulate glycogenolysis and gluconeogenesis. (A)</p> Signup and view all the answers

If a researcher discovers a new drug that increases the entropy (S) within a cancer cell, how might this drug affect the cell's metabolism, assuming enthalpy (H) remains relatively constant?

<p>It would decrease the cell's metabolic activity by increasing the Gibbs free energy (G). (D)</p> Signup and view all the answers

How does the formation of isocitrate from citrate prepare the molecule for subsequent reactions in the Krebs Cycle?

<p>It rearranges the structure to facilitate oxidation and NADH production. (B)</p> Signup and view all the answers

What is the primary role of the folds (cristae) in the inner mitochondrial membrane?

<p>To increase the surface area for oxidative phosphorylation. (B)</p> Signup and view all the answers

What is the primary role of isocitrate dehydrogenase in the Krebs Cycle?

<p>To catalyze the oxidation of isocitrate, producing NADH and CO₂. (B)</p> Signup and view all the answers

Which of the following best describes the significance of the conversion of α-ketoglutarate to succinyl-CoA?

<p>It involves decarboxylation and the addition of CoA, producing NADH. (C)</p> Signup and view all the answers

How does the electron transport chain (ETC) contribute to ATP synthesis?

<p>It releases energy from NADH and FADH₂ to pump protons and create an electrochemical gradient. (C)</p> Signup and view all the answers

Which of the following correctly describes the role of oxygen in oxidative phosphorylation?

<p>Oxygen acts as the final electron acceptor in the ETC, forming water. (A)</p> Signup and view all the answers

During the Krebs Cycle, how is GTP generated, and what is its importance?

<p>GTP is produced by the removal of CoA from succinyl-CoA and can be converted to ATP. (D)</p> Signup and view all the answers

What is the proton motive force, and how is it generated during oxidative phosphorylation?

<p>It is an electrochemical gradient of protons generated by proton pumps in the inner mitochondrial membrane. (B)</p> Signup and view all the answers

In the conversion of α-ketoglutarate to succinyl-CoA, what happens to the carbon atoms, and how is energy conserved?

<p>Carbon atoms are removed as CO₂, and energy is conserved through NADH production. (B)</p> Signup and view all the answers

How does ATP synthase utilize the proton motive force to synthesize ATP?

<p>It uses the energy from proton diffusion to rotate and catalyze ATP synthesis. (D)</p> Signup and view all the answers

Why is the regeneration of oxaloacetate crucial for the continuation of the Krebs Cycle?

<p>It is required to initially react with acetyl-CoA, restarting the cycle. (D)</p> Signup and view all the answers

During the generation of the proton motive force, what is the immediate source of energy that powers the proton pumps?

<p>The flow of electrons through the electron transport chain. (C)</p> Signup and view all the answers

How do the NADH and FADH₂ produced during the Krebs Cycle contribute to ATP synthesis?

<p>They transfer electrons to the electron transport chain, driving oxidative phosphorylation. (D)</p> Signup and view all the answers

What happens to the electrons initially carried by NADH and FADH₂ during oxidative phosphorylation?

<p>They are transferred through the electron transport chain, ultimately reducing oxygen. (A)</p> Signup and view all the answers

Which step of the Krebs Cycle directly involves substrate-level phosphorylation?

<p>Conversion of succinyl-CoA to succinate. (C)</p> Signup and view all the answers

If the inner mitochondrial membrane were made permeable to protons, what would be the most likely direct consequence?

<p>Increased oxygen consumption but decreased ATP production. (C)</p> Signup and view all the answers

What is the primary purpose of phosphorylating glucose to glucose-6-phosphate in the first step of glycolysis?

<p>To trap glucose inside the cell and destabilize it for further reactions. (A)</p> Signup and view all the answers

Why is the conversion of glucose-6-phosphate to fructose-6-phosphate important in the second step of glycolysis?

<p>Fructose-6-phosphate is more easily cleaved into two 3-carbon molecules. (D)</p> Signup and view all the answers

Phosphofructokinase (PFK) catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. What is a key regulatory aspect of this reaction in glycolysis?

<p>It commits glucose to the glycolysis pathway and is a major control point. (C)</p> Signup and view all the answers

How does the phosphorylation of glucose affect its ability to interact with transport proteins in the cell membrane?

<p>Phosphorylation decreases its affinity for glucose transporters, preventing its export from the cell, and helps maintain the glucose gradient. (D)</p> Signup and view all the answers

Which of the following best describes the role of ATP in the energy investment phase of glycolysis?

<p>ATP is hydrolyzed to provide the activation energy for certain reactions. (A)</p> Signup and view all the answers

What would be the likely outcome if a cell lacked the enzyme phosphoglucose isomerase?

<p>The cell would accumulate glucose-6-phosphate, and glycolysis would be significantly inhibited. (D)</p> Signup and view all the answers

Which of the following best describes the role of an enzyme in a chemical reaction?

<p>It speeds up the reaction rate by lowering the activation energy. (C)</p> Signup and view all the answers

How does the induced fit model describe enzyme-substrate interaction?

<p>The enzyme's active site adjusts its shape to better accommodate the substrate after the substrate binds. (D)</p> Signup and view all the answers

What is the significance of Fructose-1,6-bisphosphate formation during glycolysis concerning substrate availability for the subsequent steps?

<p>It produces a symmetrical molecule which can be readily split into two 3-carbon molecules, setting the stage for the payoff phase. (C)</p> Signup and view all the answers

Which of the following is a characteristic of competitive inhibition?

<p>The inhibitor has a structure similar to the substrate and binds to the active site. (A)</p> Signup and view all the answers

How does magnesium ($Mg^{2+}$) contribute to the function of phosphofructokinase (PFK) during glycolysis?

<p>Magnesium acts as a cofactor, stabilizing the ATP molecule's negative charges during its transfer of a phosphate group. (A)</p> Signup and view all the answers

What is the primary effect of a noncompetitive inhibitor on enzyme kinetics?

<p>It decreases the Vmax value without affecting the Km. (A)</p> Signup and view all the answers

How do cofactors and coenzymes assist enzymes in catalyzing reactions?

<p>By participating directly in the reaction by donating or accepting electrons or groups. (D)</p> Signup and view all the answers

What is the transition state in an enzyme-catalyzed reaction?

<p>An intermediate stage where the bonds of the substrate are stretched and ready to be altered. (A)</p> Signup and view all the answers

Which of the following mechanisms do enzymes use to lower activation energy?

<p>Positioning reactants closer to each other. (C)</p> Signup and view all the answers

What is the active site of an enzyme?

<p>The specific region where the substrate binds and the reaction occurs. (B)</p> Signup and view all the answers

How does pH affect enzyme activity?

<p>Extreme pH levels can cause enzymes to denature, losing their functional shape. (D)</p> Signup and view all the answers

What is the role of ribozymes in cellular processes?

<p>To catalyze reactions by acting as enzymatic RNA molecules. (C)</p> Signup and view all the answers

In a scenario where an endergonic reaction is coupled with an exergonic reaction, what condition must be met for the overall process to be spontaneous?

<p>The free energy change of the exergonic reaction must be greater than the free energy change of the endergonic reaction. (A)</p> Signup and view all the answers

Which of the following strategies would be LEAST effective for driving an endergonic reaction forward in a cell?

<p>Maintaining a high concentration of reactants to push the reaction towards product formation. (A)</p> Signup and view all the answers

What impact does ATP hydrolysis have on coupled reactions within a cell?

<p>It directly provides energy to drive endergonic reactions forward. (D)</p> Signup and view all the answers

How do cells primarily utilize ATP hydrolysis to facilitate non-spontaneous reactions?

<p>By coupling ATP hydrolysis with the non-spontaneous reaction to yield an overall negative free energy change. (B)</p> Signup and view all the answers

Consider a scenario where the product of an endergonic reaction is continuously used in a subsequent, highly exergonic reaction. How does this affect the overall spontaneity of the endergonic reaction?

<p>It increases the spontaneity of the endergonic reaction by preventing product accumulation. (A)</p> Signup and view all the answers

How do heterotrophs obtain chemical energy compared to autotrophs?

<p>Heterotrophs obtain energy by consuming organic molecules produced by other organisms, while autotrophs synthesize their own organic molecules from inorganic sources. (B)</p> Signup and view all the answers

Which of the following best describes the role of cellular respiration?

<p>To break down organic molecules to generate ATP. (B)</p> Signup and view all the answers

If a reaction has a large equilibrium constant (K), what can be inferred about the change in Gibbs free energy ($\Delta G$)?

<p>$\Delta G$ is negative, indicating a spontaneous reaction. (D)</p> Signup and view all the answers

In cellular respiration, what distinguishes aerobic respiration from anaerobic respiration?

<p>Aerobic respiration uses oxygen as the final electron acceptor, while anaerobic respiration uses an inorganic molecule other than oxygen. (A)</p> Signup and view all the answers

If a cell is actively performing glycolysis, where would you expect the highest concentration of glycolytic enzymes to be?

<p>The cell's cytosol (B)</p> Signup and view all the answers

During glycolysis, glucose is converted into pyruvate. What is the primary purpose of this conversion in the context of cellular respiration?

<p>To break down glucose into smaller molecules that can enter the mitochondria for further ATP production. (B)</p> Signup and view all the answers

How does the enzyme ATP synthase contribute to ATP production within a cell?

<p>It provides a channel for protons to move down their concentration gradient, driving ATP synthesis. (D)</p> Signup and view all the answers

After glucose enters a cell, which statement accurately describes its transport and initial modification during glycolysis?

<p>Glucose enters cells through specific transporter proteins and is converted into pyruvate in the cytosol. (D)</p> Signup and view all the answers

How does the second law of thermodynamics relate to energy transformations within a cell?

<p>Energy transformations increase the total entropy of the system and its surroundings. (C)</p> Signup and view all the answers

Which statement correctly applies the second law of thermodynamics to living organisms?

<p>Living organisms temporarily decrease their entropy locally, but increase the entropy of their surroundings. (A)</p> Signup and view all the answers

A plant synthesizes glucose during photosynthesis. How does this process relate to potential and kinetic energy?

<p>It converts kinetic energy (light) into potential energy (glucose). (C)</p> Signup and view all the answers

Which of the following cellular processes is most directly associated with the concept of kinetic energy?

<p>The movement of ions across a cell membrane (B)</p> Signup and view all the answers

What is the primary role of ATP in anabolic pathways?

<p>To provide the energy required for synthesizing complex molecules from simpler ones. (C)</p> Signup and view all the answers

In the context of cellular metabolism, how are anabolic and catabolic pathways related?

<p>Anabolic pathways build complex molecules, while catabolic pathways break them down, often providing energy for anabolism. (B)</p> Signup and view all the answers

Which of the following is an example of how potential energy is utilized in cellular processes?

<p>Using the energy stored in a glucose molecule to synthesize ATP. (A)</p> Signup and view all the answers

How does the synthesis of a protein from amino acids contribute to the overall thermodynamics of a cell?

<p>It decreases the local entropy within the cell but increases the entropy of the surroundings. (D)</p> Signup and view all the answers

Which evolutionary step was crucial in paving the way for aerobic respiration by fundamentally altering Earth's atmosphere?

<p>Oxygen-forming photosynthesis (A)</p> Signup and view all the answers

How did the evolution of oxygen-producing photosynthesis impact the development of life on Earth?

<p>It made energy production more efficient, supporting complex life forms. (B)</p> Signup and view all the answers

Which of the following metabolic processes is the most ancient and still universally conserved across all domains of life?

<p>Glycolysis (D)</p> Signup and view all the answers

How does aerobic respiration enhance energy production compared to earlier metabolic processes?

<p>It produces more ATP per glucose molecule. (A)</p> Signup and view all the answers

Which of the following metabolic pathways provides precursor molecules that cells use as building blocks for anabolic reactions?

<p>Glycolysis and the Citric Acid Cycle (C)</p> Signup and view all the answers

How do cells use the intermediates produced during glycolysis and the citric acid cycle?

<p>To synthesize larger molecules needed for growth and repair (C)</p> Signup and view all the answers

Which of these molecules is NOT directly derived as an intermediate from the citric acid cycle for anabolic processes?

<p>Fructose-6-phosphate (D)</p> Signup and view all the answers

How does the anabolic use of intermediates from glycolysis and the citric acid cycle contribute to cellular function?

<p>It supports cell growth, repair, and energy storage. (C)</p> Signup and view all the answers

What is the primary purpose of rearranging citrate to form isocitrate in the Krebs Cycle?

<p>To prepare the molecule for subsequent oxidation and NADH production. (D)</p> Signup and view all the answers

During the conversion of isocitrate to α-ketoglutarate, what is the immediate fate of the released carbon atom?

<p>It is released as carbon dioxide (CO₂). (C)</p> Signup and view all the answers

How does the conversion of α-ketoglutarate to succinyl-CoA contribute to the electron transport chain (ETC)?

<p>It reduces NAD⁺ to NADH + H⁺, which carries electrons to the ETC. (C)</p> Signup and view all the answers

What type of phosphorylation occurs during the conversion of succinyl-CoA to succinate, and why is it important?

<p>Substrate-level phosphorylation; it directly generates GTP, which can be converted to ATP. (C)</p> Signup and view all the answers

During which reaction of the citric acid cycle is FADH2 formed directly?

<p>Conversion of succinate to fumarate. (C)</p> Signup and view all the answers

Which molecule acts as the initial substrate by accepting acetyl-CoA to begin the Krebs Cycle?

<p>Oxaloacetate (B)</p> Signup and view all the answers

Which of these components is regenerated at the end of Krebs cycle?

<p>Oxaloacetate (C)</p> Signup and view all the answers

What is the direct result of the phosphoglycerate kinase reaction in glycolysis?

<p>Production of two ATP molecules per glucose molecule. (C)</p> Signup and view all the answers

Which type of enzyme is phosphoglycerate mutase (PGM)?

<p>A mutase that relocates functional groups within a molecule. (B)</p> Signup and view all the answers

What is the main function of enolase in glycolysis?

<p>To remove a water molecule from 2-phosphoglycerate, forming phosphoenolpyruvate. (A)</p> Signup and view all the answers

How does the activity of phosphoglycerate kinase directly contribute to the energy payoff phase of glycolysis?

<p>By generating ATP through substrate-level phosphorylation. (D)</p> Signup and view all the answers

What is the primary role of phosphoglycerate mutase in preparing 3-phosphoglycerate for the next step in glycolysis?

<p>It rearranges the phosphate group to a position where it can be more easily removed. (D)</p> Signup and view all the answers

How does the reaction catalyzed by enolase contribute to the overall process of glycolysis?

<p>It increases the potential energy of the substrate by creating a high-energy phosphate bond. (B)</p> Signup and view all the answers

Which statement describes the relationship between the reactions catalyzed by phosphoglycerate kinase and enolase?

<p>They occur sequentially, with the product of phosphoglycerate kinase serving as the substrate for enolase. (D)</p> Signup and view all the answers

If a cell were deficient in phosphoglycerate mutase, how would it impact the later steps of glycolysis?

<p>The formation of 2-phosphoglycerate, a substrate for enolase, would be reduced. (A)</p> Signup and view all the answers

How do chloroplasts facilitate the process of photosynthesis?

<p>By capturing sunlight with pigments in thylakoid membranes for ATP production and using CO₂ in the stroma for building organic molecules. (A)</p> Signup and view all the answers

What is the functional relationship between the light-dependent and light-independent reactions in photosynthesis?

<p>The light-dependent reactions provide ATP and NADPH, which are then used by the light-independent reactions to fix CO₂ into organic molecules. (C)</p> Signup and view all the answers

How does the electromagnetic spectrum relate to photosynthesis, and what implications does this relationship have?

<p>Photosynthesis utilizes specific wavelengths within the visible light spectrum, and UV light can be harmful due to its high energy. (A)</p> Signup and view all the answers

If a plant species had a mutation that reduced the amount of chlorophyll b it produced, how would this likely affect its photosynthetic efficiency?

<p>It would decrease, especially in light wavelengths that chlorophyll b normally absorbs. (B)</p> Signup and view all the answers

What role do carotenoids play in photosynthesis, and how do they contribute to the overall process?

<p>Carotenoids mainly capture and transfer light energy to chlorophyll and protect against excessive light damage. (A)</p> Signup and view all the answers

How does the alternating carbon-bond system in chlorophyll facilitate the excitation and channeling of electrons during photosynthesis?

<p>It provides a pathway for excited electrons to be delocalized and channeled away for use in subsequent reactions. (B)</p> Signup and view all the answers

How would a decrease in the number of stomata on the leaf surface of a plant most likely affect photosynthesis and plant function?

<p>It would reduce CO₂ uptake, potentially decreasing photosynthetic rates and increasing water retention. (D)</p> Signup and view all the answers

What would be the most likely direct effect of a toxin that inhibits the formation of grana within the chloroplast?

<p>Decreased efficiency of light-dependent reactions due to reduced surface area for pigment organization. (B)</p> Signup and view all the answers

During periods of cellular growth and repair, how does anabolism primarily contribute to these processes?

<p>By synthesizing larger molecules and cellular structures from smaller precursors. (B)</p> Signup and view all the answers

Which of the following is an example of how anabolism uses ATP to drive biosynthetic reactions?

<p>Phosphorylating metabolic intermediates, increasing their potential energy for subsequent reactions. (C)</p> Signup and view all the answers

How do cells primarily regulate anabolic pathways to maintain energy balance, preventing wasteful overproduction?

<p>Through hormonal signals and feedback inhibition, adjusting enzyme activity based on energy availability and product accumulation. (C)</p> Signup and view all the answers

In the context of anabolic reactions, what is the role of NADPH, and why is it important?

<p>It provides reducing power by donating electrons for biosynthetic reactions, such as fatty acid synthesis. (D)</p> Signup and view all the answers

If a cell has sufficient energy reserves and high levels of anabolic intermediates, which regulatory mechanism would most likely be activated to slow down anabolism?

<p>Feedback inhibition, where the end-products of anabolic pathways inhibit the enzymes involved in their synthesis. (C)</p> Signup and view all the answers

During oxidative phosphorylation, what is the direct role of the electron transport chain (ETC)?

<p>To use energy from electrons to pump protons across the inner mitochondrial membrane, creating an electrochemical gradient. (C)</p> Signup and view all the answers

How does the folding of the inner mitochondrial membrane (cristae) directly contribute to the efficiency of ATP production?

<p>By increasing the surface area available for the electron transport chain and ATP synthase complexes. (A)</p> Signup and view all the answers

What is the immediate consequence if the flow of electrons through the electron transport chain is completely inhibited?

<p>The proton gradient across the inner mitochondrial membrane would dissipate, halting ATP synthesis. (D)</p> Signup and view all the answers

During oxidative phosphorylation, what directly powers the movement of protons (H⁺) from the mitochondrial matrix to the intermembrane space?

<p>The energy released during the oxidation of NADH and FADH₂ as electrons are passed along the ETC. (B)</p> Signup and view all the answers

Which event would most directly decrease the amount of ATP produced via chemiosmosis?

<p>An increase in the permeability of the inner mitochondrial membrane to protons. (A)</p> Signup and view all the answers

What is the primary role of oxygen in the process of oxidative phosphorylation?

<p>To serve as the final electron acceptor in the electron transport chain, forming water. (D)</p> Signup and view all the answers

If a cell were treated with a drug that inhibits ATP synthase, what would be the immediate effect on the electron transport chain?

<p>The electron transport chain would slow down or halt due to the buildup of the proton gradient. (B)</p> Signup and view all the answers

How does NADH contribute to the generation of ATP during oxidative phosphorylation differently than FADH₂?

<p>NADH enters the electron transport chain at a complex that pumps more protons, resulting in more ATP. (C)</p> Signup and view all the answers

Which characteristic is most indicative of a reaction that requires an additional driving force to proceed?

<p>It has a standard change in free energy that is positive. (B)</p> Signup and view all the answers

How do enzymes increase the rate of a biochemical reaction?

<p>By lowering the activation energy of the reaction. (A)</p> Signup and view all the answers

What is the significance of the transition state in an enzyme-catalyzed reaction?

<p>It represents the point where bonds in the reactants are stretched and ready to be rearranged. (B)</p> Signup and view all the answers

Which of the following mechanisms is NOT a way enzymes lower activation energy?

<p>Decreasing the local temperature. (B)</p> Signup and view all the answers

How does the 'induced fit' model enhance enzyme specificity?

<p>By causing a conformational change in the enzyme that optimizes the fit for the substrate. (A)</p> Signup and view all the answers

Which of the following is a characteristic of competitive inhibition in enzyme reactions?

<p>The inhibitor binds directly to the active site, preventing substrate binding. (B)</p> Signup and view all the answers

Noncompetitive inhibitors reduce enzyme activity by which mechanism?

<p>Altering the enzyme's conformation, thereby reducing Vmax. (D)</p> Signup and view all the answers

What is the role of cofactors and coenzymes in enzyme function?

<p>They assist enzymes in catalyzing reactions. (D)</p> Signup and view all the answers

How does temperature generally affect enzyme activity, assuming all other conditions are optimal?

<p>Enzyme activity typically increases with temperature to an optimum, then decreases sharply due to denaturation. (A)</p> Signup and view all the answers

How do ribozymes catalyze biological reactions?

<p>By using RNA molecules with catalytic properties. (C)</p> Signup and view all the answers

Which of the following best describes the role of enzymes in metabolic pathways?

<p>They catalyze specific reactions, facilitating metabolic steps. (B)</p> Signup and view all the answers

How does the equation $\Delta G = \Delta H - T\Delta S$ relate to the spontaneity of a reaction?

<p>A negative $\Delta G$ indicates a spontaneous reaction, meaning it occurs without needing external energy. (A)</p> Signup and view all the answers

What is the role of photosynthesis in the context of energy for most life forms?

<p>It utilizes sunlight to synthesize biological molecules. (A)</p> Signup and view all the answers

In a scenario where both the change in enthalpy ($\Delta H$) is positive and the change in entropy ($\Delta S$) is positive for a reaction, how would increasing the temperature (T) affect the spontaneity of the reaction?

<p>Increase in temperature might make it spontaneous. (A)</p> Signup and view all the answers

How do hormones like glucagon influence catabolic pathways during periods of low energy availability?

<p>They promote catabolic pathways, such as glycogenolysis and lipolysis. (B)</p> Signup and view all the answers

If a reaction is at equilibrium, what does this indicate about the Gibbs free energy ($\Delta G$)?

<p>$\Delta G$ is equal to zero. (D)</p> Signup and view all the answers

How does heat affect the Gibbs free energy ($G$) of a reaction, especially considering the relationship $G = H - TS$?

<p>Increasing heat (temperature) can increase the importance of entropy in determining reaction spontaneity. (C)</p> Signup and view all the answers

How do cells typically couple ATP hydrolysis to non-spontaneous reactions in order to drive them forward?

<p>By using the energy released from ATP hydrolysis to reduce the enthalpy of the reaction (C)</p> Signup and view all the answers

What is the immediate result of phosphoglycerate kinase (PGK) activity during glycolysis?

<p>Production of two ATP molecules per glucose molecule processed. (B)</p> Signup and view all the answers

If phosphoglycerate kinase (PGK) is inhibited, which of the following metabolites would likely accumulate?

<p>1,3-bisphosphoglycerate (A)</p> Signup and view all the answers

What is the specific function of phosphoglycerate mutase in glycolysis?

<p>To relocate a phosphate group within the 3-phosphoglycerate molecule. (C)</p> Signup and view all the answers

During the energy investment phase of glycolysis, what is the primary role of ATP?

<p>To phosphorylate glucose and fructose-6-phosphate, increasing their potential energy. (A)</p> Signup and view all the answers

Why is the isomerization of glucose-6-phosphate to fructose-6-phosphate a crucial step in glycolysis?

<p>It converts a six-membered ring into a five-membered ring, enabling easier cleavage in later steps. (B)</p> Signup and view all the answers

If a cell were deficient in phosphoglycerate mutase, how would this directly impact the subsequent steps of glycolysis?

<p>It would slow down the production of 2-phosphoglycerate, affecting downstream reactions. (A)</p> Signup and view all the answers

In the third step of glycolysis, phosphofructokinase (PFK) catalyzes the addition of a phosphate group to fructose-6-phosphate. What is the significance of this step?

<p>It commits glucose to the glycolytic pathway and is a major regulatory point. (A)</p> Signup and view all the answers

What type of reaction is catalyzed by the Enzyme Enolase?

<p>Dehydration (A)</p> Signup and view all the answers

Which molecule is formed as a direct result of the action of enolase during glycolysis?

<p>Phosphoenolpyruvate (PEP) (D)</p> Signup and view all the answers

What is the role of magnesium ($Mg^{2+}$) as a cofactor in the phosphofructokinase (PFK) reaction during glycolysis?

<p>It stabilizes the ATP molecule within the enzyme's active site, facilitating phosphate transfer. (C)</p> Signup and view all the answers

Following the action of hexokinase in glycolysis, why is it important that glucose is quickly converted to glucose-6-phosphate (G6P)?

<p>The addition of the phosphate traps glucose inside the cell and destabilizes it, facilitating further metabolism. (A)</p> Signup and view all the answers

How does the unique structure of the enzyme enolase facilitate its function?

<p>By providing a specific enzyme pocket that allows a series of steps to occur. (C)</p> Signup and view all the answers

If enolase were inhibited in a cell, which of the following outcomes would be most likely?

<p>Accumulation of 2-phosphoglycerate. (C)</p> Signup and view all the answers

If a cell were treated with a drug that inhibits phosphoglucose isomerase, which of the following metabolites would likely accumulate?

<p>Glucose-6-phosphate (B)</p> Signup and view all the answers

How does the highly exergonic nature of the phosphofructokinase (PFK) reaction contribute to the regulation of glycolysis?

<p>It makes the reaction virtually irreversible under cellular conditions, committing the cell to glycolysis. (D)</p> Signup and view all the answers

What is the net ATP production directly resulting from the reactions of glycolysis (not including ATP derived from subsequent processes like oxidative phosphorylation)?

<p>2 ATP (A)</p> Signup and view all the answers

Flashcards

Fatty Acids

Long hydrocarbon chains with a carboxylic acid group, components of lipids and energy storage molecules.

Amino Acids

Organic molecules that are building blocks of proteins, containing an amino group, a carboxyl group, and a unique side chain (R group).

Energy Carriers

Molecules that store and transport energy within cells for metabolic processes like ATP, NADH, and FADH2.

Glycolysis

Metabolic pathway that converts glucose to pyruvate, producing a small amount of ATP and NADH.

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Citric Acid Cycle (Krebs Cycle)

Series of enzyme-catalyzed reactions in the mitochondria that oxidizes Acetyl-CoA to produce NADH, FADH2, GTP, and CO2.

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Oxidative Phosphorylation

Final step of cellular respiration in the inner mitochondrial membrane, where electrons from NADH and FADH2 produce ATP and water.

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Beta-Oxidation

Catabolic process in mitochondria breaking down fatty acids into Acetyl-CoA for ATP production via the Citric Acid Cycle.

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Catabolism's Role

Breaks down complex molecules into simpler ones, releasing energy captured as ATP for cellular processes.

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Hormonal Control of Catabolism

Hormones regulate catabolic activity, promoting breakdown (e.g., glycogenolysis, lipolysis) in low-energy states and inhibiting it when energy is sufficient.

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Feedback Mechanisms in Metabolism

Regulatory processes that maintain homeostasis by stimulating or inhibiting metabolic pathways based on metabolite concentrations and energy availability.

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Catabolism vs. Anabolism

Catabolism: breakdown of complex molecules to release energy. Anabolism: building complex molecules from simpler ones, requiring energy.

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Significance of Catabolism

Catabolism provides the energy needed for essential processes necessary for cell survival and function.

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Catabolism During Scarcity

Cells break down stored molecules (like glycogen or fats) during scarcity to mobilize energy reserves quickly.

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Metabolism Definition

A series of enzyme-catalyzed chemical reactions that convert food into energy and build cellular structures.

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Enzymes in Metabolism

Proteins that catalyze each step in metabolic pathways, making reactions occur efficiently.

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G = H - TS Key Variables

Change in free energy (usable energy), enthalpy (total energy), temperature, and entropy (disorder).

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Change in Enthalpy (H)

Represents the total energy of a system, including usable energy and heat.

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Significance of Temperature (T)

Affects the entropy (S) of a system; higher temperatures increase randomness.

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Change in Entropy (S)

The degree of disorder or randomness in a system; higher values mean greater disorder.

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Spontaneous Reactions

Occur spontaneously when the change in free energy (G) is negative.

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Gibbs Free Energy (G)

Indicates the amount of energy available to do work in a system at constant temperature and pressure.

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'H' in Thermodynamics

Enthalpy or total energy; accounts for internal energy and energy required to make room for a system.

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'S' in Thermodynamics

A measure of disorder or the amount of energy unavailable to do work.

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Glucose Breakdown

Releases energy, resulting in a negative Gibbs free energy (G)

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Negative Gibbs Free Energy (G)

A reaction that can occur spontaneously releasing energy.

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Positive Gibbs Free Energy (G)

A reaction that requires energy input to proceed.

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Entropy (S)

A measure of disorder; higher entropy means more unusable energy.

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Second Law of Thermodynamics (Entropy)

The total entropy of a closed system always increases.

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ATP Hydrolysis

ATP breaks down into ADP and inorganic phosphate releasing energy.

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Thermodynamic Equilibrium (Gibbs)

The Gibbs free energy is minimized and there is no net change.

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ATP's Role in the Cell

The primary energy currency of the cell, powers cellular processes.

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Enthalpy (H) and Biomolecule Formation

Requires energy input, often supplied by ATP hydrolysis.

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Anabolism

The set of metabolic pathways that construct molecules from smaller units.

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Importance of Anabolism

Building tissues, expanding cell size, replacing damaged molecules/organelles, and synthesizing energy-rich molecules.

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Regulation of Anabolic Pathways

Cells balance energy expenditure with availability regulated by hormones (like insulin) and feedback inhibition.

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Catabolism

The breakdown of complex molecules into simpler ones as part of cellular metabolism.

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Nature of Catabolic Reactions

Catabolic reactions release energy when complex molecules are broken down.

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Molecules in Catabolic Reactions

Carbohydrates, lipids, and proteins, which are broken down to simpler molecules.

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Energy-Rich Molecules Significance

Stored energy that can be accessed when the body requires energy.

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Feedback Inhibition

End-products of anabolic pathways inhibit enzymes involved in their own synthesis.

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Viral Capsid Function

Protects the viral genome and delivers it to host cells.

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Capsid Shapes

Icosahedral, helical, or complex.

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Capsids in Nano-biotech

Studying protein dynamics and energy transfer in viral infections.

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Capsid vs. Capsomere

Capsid: complete shell; Capsomere: subunit of the shell.

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Microtubules

Key cytoskeletal component made of tubulin, involved in cell structure and function.

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Polymerization/Depolymerization

Assembly of tubulin into microtubules; disassembly of microtubules into tubulin.

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Microtubule Functions

Structural support, intracellular transport, cell division, and movement.

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Microtubule Energy Relation

Motor proteins use ATP to move cargo along microtubules, dynamic instability enables cell movements.

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Glucagon and Cortisol

Promote the breakdown of molecules (catabolism) during periods of low energy, like stimulating glycogenolysis (glycogen breakdown) and lipolysis (fat breakdown).

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Enzymes

Proteins that facilitate (catalyze) each reaction step in metabolic pathways.

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Photosynthesis

The process of using sunlight to build biological molecules (like sugars).

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∆G

Change in free energy, which indicates the amount of energy available to do work.

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∆H

Change in enthalpy, is the total energy of a system, including usable energy and heat.

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T

Temperature, measured in Kelvin, which impacts entropy.

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∆S

Change in entropy means change in disorder or randomness within a system.

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Endergonic Reaction

A chemical reaction requiring an additional driving force due to a positive change in free energy.

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Catalyst

An agent, like a protein or RNA, that speeds up a chemical reaction without being consumed.

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Ribozymes

RNA molecules with catalytic properties.

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Activation Energy

The initial energy needed to start a chemical reaction.

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Active Site

The location on an enzyme where the reaction takes place.

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Substrates

The reactants that bind to the active site of an enzyme.

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Enzyme-Substrate Complex

The complex formed when an enzyme and substrate bind.

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Competitive Inhibition

Molecule binds to enzyme, inhibiting substrate binding; increasing apparent KM.

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Noncompetitive Inhibition

Inhibitor binds to enzyme, reducing Vmax without affecting KM; binds away from the active site.

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Running Endergonic Rxn

Couple with exergonic rxn, low product concentration, or molecular machine.

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Endergonic Coupled Reactions

Change in free energy must be negative for both processes.

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Equilibrium Constant

Relative reactant/product concentration depends on Gibbs Free Energy.

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Autotrophs

Organisms that produce their own food from inorganic substances using light or chemical energy.

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Heterotrophs

Organisms that obtain energy by consuming organic substances.

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Cellular Respiration

Process convert chem energy in food to usable cellular energy (ATP).

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Energy Investment Phase

The first phase of glycolysis where ATP is used to prepare glucose for breakdown.

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Energy Payoff Phase

The second phase of glycolysis where ATP and NADH are produced.

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Glycolysis End Products

Two molecules of pyruvate are yielded. A net production of 2 ATP. 2 NADH electron carriers are produced.

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Glycolysis Step 1 Purpose

Glucose is phosphorylated to form Glucose-6-Phosphate (G6P), trapping glucose inside the cell and making it more reactive.

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Glycolysis Step 1 Enzyme

Hexokinase catalyzes the phosphorylation of glucose to form Glucose-6-Phosphate (G6P).

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Glycolysis Step 2 Reaction

Glucose-6-Phosphate is rearranged into Fructose-6-Phosphate.

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Glycolysis Step 2 Enzyme

Phosphoglucose Isomerase rearranges glucose 6-phosphate (G6P) into fructose 6-phosphate.

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Glycolysis Step 3 Purpose

Fructose-6-Phosphate is phosphorylated to form Fructose-1,6-Bisphosphate, committing glucose to glycolysis.

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Krebs Cycle Initiation

The starting reaction of the Krebs Cycle for energy production.

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Citrate to Isocitrate

Citrate is rearranged into isocitrate by aconitase.

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Isocitrate Oxidation

Isocitrate is oxidized to α-ketoglutarate.

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Isocitrate Dehydrogenase

Isocitrate dehydrogenase oxidizes isocitrate, producing NADH and CO₂.

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α-Ketoglutarate Conversion

α-Ketoglutarate is converted to succinyl-CoA.

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α-Ketoglutarate dehydrogenase

α-Ketoglutarate dehydrogenase reduces NAD⁺ to NADH + H⁺.

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Succinyl-CoA to Succinate

Succinyl-CoA is converted to succinate.

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GTP Formation

The energy released from succinyl-CoA to succinate to form GTP from GDP

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Cristae

Folds in the inner mitochondrial membrane to increase surface area for ATP production.

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Electron Transport Chain (ETC) Role

Transfers electrons from NADH & FADH₂ to ATP. Oxygen is the final electron acceptor, forming H₂O.

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Oxidative Phosphorylation Steps

Proton pumps create this gradient. ATP synthase uses it (chemiosmosis) to synthesise ATP. Oxygen accepts electrons and protons to form water.

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Proton Pumping & Electrochemical Gradient Formation

NADH and FADH₂ transfer electrons, powering proton pumps and creating a proton motive force.

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ATP Synthesis via Chemiosmosis

ATP synthase allows protons to diffuse back, powering ATP synthesis from ADP and Pi.

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Oxygen's Role

O₂ accepts electrons and protons, forming water (H₂O).

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Oxidation of NADH & FADH₂

NADH and FADH₂ release high-energy electrons and protons (H⁺).

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Aerobic Respiration

Uses oxygen as the final electron acceptor.

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Anaerobic Respiration

Uses an inorganic molecule (not oxygen) as the final electron acceptor.

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Fermentation

Uses an organic molecule as the final electron acceptor.

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ATP Synthase

The enzyme that produces most ATP in cells by using a proton gradient to drive a rotary motor.

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Potential Energy

Energy stored in an object due to its position or condition.

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Kinetic Energy

Energy of motion.

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Second Law of Thermodynamics

In any energy transfer, the total entropy (disorder) of a system and its surroundings always increases.

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Aconitase Function

Rearranges citrate to form isocitrate.

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Isocitrate Importance

Key intermediate in the Krebs Cycle.

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Isocitrate Oxidation Reaction

Oxidation of isocitrate to form α-ketoglutarate. Generates NADH and releases CO₂.

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α-Ketoglutarate Role

Oxidized to form succinyl-CoA, releasing CO₂ and producing NADH.

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Succinyl-CoA Conversion

Succinyl-CoA is transformed into succinate.

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Succinyl-CoA Synthase

The enzyme catalyzes the conversion of succinyl-CoA to succinate.

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Degradation (Early Life)

Breaking down organic molecules for energy without oxygen.

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Anaerobic Photosynthesis

Using light energy to fix carbon without producing oxygen.

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Oxygen-Forming Photosynthesis

Producing oxygen as a byproduct of photosynthesis, leading to the Great Oxygenation Event.

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Nitrogen Fixation

Converting atmospheric nitrogen into usable forms.

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Anabolic Pathways

Cells construct molecules from smaller precursors, involving glycolysis and the citric acid cycle.

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Glycolysis & Citric Acid Cycle Intermediates

Intermediates allow cells to grow, repair, and store energy.

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Thylakoid Membranes

Internal membranes within the chloroplasts where light-dependent reactions occur.

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Photosystems

Clusters of pigment molecules in the thylakoid membrane that capture light energy.

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Phosphoglycerate Kinase Function

Transfers a phosphate group from 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate.

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Light-Dependent Reactions

Sunlight is captured and converted to ATP and NADPH.

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Net ATP Production (Step 7)

Produces 2 ATP molecules per glucose molecule.

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Phosphoglycerate Mutase Function

Rearranges the phosphate group on 3-phosphoglycerate from the 3rd carbon to the 2nd carbon, forming 2-phosphoglycerate.

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Light-Independent Reactions (Calvin Cycle)

CO₂ is used to build organic molecules (sugars), using ATP and NADPH from the light reactions.

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Mutase

Enzymes that catalyze the transfer of a functional group from one position on a molecule to another.

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Photon

The packet of energy of electromagnetic radiation.

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Pigments

Molecules that absorb light in the visible range of the electromagnetic spectrum.

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Enolase Function

Removes a molecule of water (dehydrates) from 2-phosphoglycerate to form phosphoenolpyruvate (PEP).

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Enolase Reaction

Conversion of 2-phosphoglycerate to phosphoenolpyruvate (PEP) and removes a water molecule.

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Chlorophyll

Main pigment in plants that absorbs light to provide energy for photosynthesis.

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Enolase

An enzyme that catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate (PEP).

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Phosphoglycerate Kinase Reaction

Conversion of 1,3 bisphoglycerate is converted to 3-phosphoglycerate

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Anabolism Definition

Metabolic processes that require energy to synthesize complex molecules from simpler ones.

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Anabolism Products

Proteins, nucleic acids, lipids and carbohydrates.

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ATP's Role in Anabolism

Supplies energy for molecule synthesis by phosphorylating intermediates.

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NADPH Role in Anabolism

Provides reducing power in biosynthetic reactions (e.g., fatty acid synthesis).

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Anabolism Crucial For:

Building tissues/expanding cell size, repairing damage, and synthesizing energy-rich molecules (glycogen/triglycerides).

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Cristae Function

Inner mitochondrial membrane folds that increase surface area for ATP production.

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ETC's Role

Converts high-energy electrons from NADH & FADH₂ into ATP. Oxygen is the final electron acceptor, forming water.

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Electrochemical Gradient Creation

NADH and FADH₂ transfer electrons, powering proton pumps and creating a proton motive force.

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ATP Synthase in Chemiosmosis

ATP synthase allows protons to diffuse back, powering ATP synthesis from ADP and Pi.

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Oxygen in ETC

O₂ accepts electrons and protons, forming water (H₂O).

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Phosphoglycerate Kinase (PGK)

Enzyme in glycolysis that transfers a phosphate group from 1,3-bisphosphoglycerate to ADP, forming ATP and 3-phosphoglycerate.

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ATP Production (Step 7)

For each glucose molecule that enters glycolysis, this step produces two ATP molecules.

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Phosphoglycerate Mutase

Enzyme that relocates the phosphate group in 3-phosphoglycerate from the 3rd carbon to the 2nd carbon, forming 2-phosphoglycerate.

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Phosphoglycerate Mutase (PGM) Function

Glycolysis enzyme that catalyzes the conversion of 3-phosphoglycerate to 2-phosphoglycerate.

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2-Phosphoglycerate

Substrate of enolase found in step 9 of glycolysis

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Phosphoenolpyruvate (PEP)

Product from enolase in step 9 of glycolysis

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