Cellular respiration

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Questions and Answers

Which molecule is NOT considered an electron carrier?

  • FAD
  • ATP (correct)
  • NAD+
  • NAD

What role do NAD and FAD primarily serve in metabolic processes?

  • They regulate cellular respiration rates.
  • They accept or donate high energy electrons. (correct)
  • They act as catalysts for biochemical reactions.
  • They act as energy storage molecules.

What happens to NAD+ after it accepts electrons during the breakdown of glucose?

  • It is released as waste.
  • It becomes NADH. (correct)
  • It converts into ATP.
  • It gets transformed into glucose.

High energy electrons are produced during which process?

<p>The breakdown of glucose. (C)</p> Signup and view all the answers

Which of the following statements about electron carriers is true?

<p>They are specific to certain types of reactions. (B)</p> Signup and view all the answers

What is the primary role of the Electron Transfer Chain (ETC) in cellular metabolism?

<p>To utilize high energy electrons for ATP production (C)</p> Signup and view all the answers

Which complexes in the Electron Transfer Chain are classified as proton pumps?

<p>Complex I, II, III, IV (A)</p> Signup and view all the answers

What is the function of ATP synthase in cellular respiration?

<p>To convert ADP to ATP using proton gradient energy (B)</p> Signup and view all the answers

How many ATP molecules can be produced from one molecule of glucose during cellular respiration?

<p>30-32 ATP (A)</p> Signup and view all the answers

What key role does the citric acid cycle play in ATP production?

<p>It generates high energy electron carriers (B)</p> Signup and view all the answers

What is a major source of fuel in diets according to the content?

<p>Glucose (B)</p> Signup and view all the answers

Which factor is NOT mentioned as affecting cellular metabolism?

<p>Environmental temperature (D)</p> Signup and view all the answers

What role do digestive enzymes play in metabolism?

<p>They facilitate the breakdown of food into basic components. (A)</p> Signup and view all the answers

Which of the following is classified as a metabolic disease?

<p>Diabetes (D)</p> Signup and view all the answers

What occurs when there are faults in enzymes related to metabolism?

<p>Production of unwanted by-products (C)</p> Signup and view all the answers

Which of the following is considered a basic component of digested food?

<p>Lipids (A)</p> Signup and view all the answers

How does malnutrition impact metabolism?

<p>Disrupts normal metabolic processes (A)</p> Signup and view all the answers

What is a potential clinical issue with abnormal lactate generation?

<p>Critical impact on blood pH (A)</p> Signup and view all the answers

What are metabolites primarily converted from?

<p>Fats and carbohydrates (A)</p> Signup and view all the answers

Which of the following diseases is NOT specifically listed as impacting metabolism?

<p>Celiac disease (D)</p> Signup and view all the answers

How many ATP molecules are produced from glycolysis?

<p>2 (B)</p> Signup and view all the answers

What is the total amount of ATP produced from the electron transfer phase?

<p>26 (A)</p> Signup and view all the answers

What is the combined total of ATP produced when glycolysis, citric acid cycle, and electron transfer are summed?

<p>30 (D)</p> Signup and view all the answers

Which component of metabolism is primarily responsible for the complexity mentioned?

<p>Biochemistry (C)</p> Signup and view all the answers

Which of the following textbooks is recommended for advanced biochemistry?

<p>Berg/Stryer – THE biochemistry textbook (C)</p> Signup and view all the answers

What primary molecule is produced from the oxidation of pyruvate in the mitochondria?

<p>Acetyl CoA (D)</p> Signup and view all the answers

Which enzyme is responsible for the conversion of pyruvate to acetyl CoA?

<p>Pyruvate Dehydrogenase (B)</p> Signup and view all the answers

Where does the conversion of pyruvate to acetyl CoA occur?

<p>Mitochondrial Matrix (D)</p> Signup and view all the answers

What cycle does acetyl CoA enter after its formation?

<p>Citric Acid Cycle (D)</p> Signup and view all the answers

What are the main high-energy electron carriers produced in the Citric Acid Cycle?

<p>NADH and FADH2 (B)</p> Signup and view all the answers

Which metabolic process continuously produces pyruvate for the Citric Acid Cycle?

<p>Glycolysis (A)</p> Signup and view all the answers

What type of reaction occurs when pyruvate is converted to acetyl CoA?

<p>Decarboxylation (A)</p> Signup and view all the answers

What happens to the CO2 produced during the Citric Acid Cycle?

<p>It is released as a waste product. (D)</p> Signup and view all the answers

What is the relationship between glycolysis and the Citric Acid Cycle?

<p>Glycolysis produces pyruvate that feeds into the Citric Acid Cycle. (D)</p> Signup and view all the answers

What is another name for the Citric Acid Cycle?

<p>Tricarboxylic Acid Cycle (C)</p> Signup and view all the answers

What is the primary substrate used in glycolysis?

<p>Glucose (B)</p> Signup and view all the answers

Which product is formed at the end of glycolysis?

<p>Pyruvate (C)</p> Signup and view all the answers

What phase of glycolysis requires the input of ATP?

<p>Preparatory phase (C)</p> Signup and view all the answers

During which phase of glycolysis is ATP produced?

<p>Payoff phase (C)</p> Signup and view all the answers

What type of phosphorylation occurs during the ATP generation in glycolysis?

<p>Substrate-level phosphorylation (A)</p> Signup and view all the answers

How many chemical reactions are involved in glycolysis?

<p>10 (D)</p> Signup and view all the answers

Which molecule is converted to G3P during glycolysis?

<p>Dihydroxyacetone phosphate (DHAP) (B)</p> Signup and view all the answers

What process connects glycolysis to the next stage of cellular respiration?

<p>Pyruvate processing (B)</p> Signup and view all the answers

In which cellular location does glycolysis occur?

<p>Cytoplasm (B)</p> Signup and view all the answers

What is the fate of the phosphate group during the conversion of PEP to pyruvate?

<p>It is transferred to ADP to form ATP. (C)</p> Signup and view all the answers

What intermediate product is generated in the conversion of glucose during glycolysis?

<p>Fructose-1,6-bisphosphate (D)</p> Signup and view all the answers

Which of the following statements about the two major stages of glycolysis is true?

<p>Only the second stage produces ATP. (A)</p> Signup and view all the answers

What is the purpose of glycolysis in cellular metabolism?

<p>Generate pyruvate for further metabolism (D)</p> Signup and view all the answers

What molecule is produced during the payoff phase of glycolysis other than ATP?

<p>NADH (D)</p> Signup and view all the answers

What role do enzymes play in the glycolytic process?

<p>Catalyze chemical reactions (A)</p> Signup and view all the answers

Flashcards

Electron Carrier

A molecule capable of accepting or donating electrons during metabolic reactions. Effectively transfers energy stored in electrons.

NAD+

A coenzyme derived from vitamin B3 (niacin) that plays a crucial role in cellular respiration, particularly in glycolysis and the citric acid cycle. It accepts electrons and becomes NADH, carrying energy.

FAD

A cofactor derived from vitamin B2 (riboflavin) that carries electrons and hydrogen atoms. It is involved in the electron transport chain, a crucial stage of cellular respiration.

Oxidation

A redox reaction that involves the loss of electrons. Energy is released through this process.

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Reduction

A redox reaction that involves the gain of electrons. Energy is absorbed through this process.

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Metabolism

The chemical processes that occur within living organisms to maintain life.

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Catabolism

The breakdown of large molecules into smaller ones, releasing energy.

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Anabolism

The synthesis of larger molecules from smaller ones, requiring energy.

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Glucose

A simple sugar that is the primary fuel source for most cells.

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Carbohydrate digestion

The process of breaking down carbohydrates into glucose.

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Fat digestion

The process of breaking down fats into fatty acids and glycerol.

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Protein digestion

The process of breaking down proteins into amino acids.

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Metabolic disorders

The study of how metabolism is affected by diseases.

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Pharmacogenetics

The study of how drugs and other substances affect metabolism.

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Homeostasis

The ability of a living organism to adapt to its environment.

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Glycolysis

The process of breaking down glucose into pyruvate, producing a small amount of ATP and NADH.

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Citric Acid Cycle

A series of reactions that occur in the mitochondria, oxidizing pyruvate and generating NADH, FADH2, and ATP.

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Electron Transfer Chain (ETC)

A process that uses the energy stored in high-energy electrons from NADH and FADH2 to generate a large amount of ATP.

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ATP Synthase

The enzyme that uses the proton gradient across the inner mitochondrial membrane to produce ATP.

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ATP Yield from Glucose

The total amount of ATP produced from one glucose molecule during cellular respiration is around 32 ATP.

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What is pyruvate?

Pyruvate is a molecule produced during glycolysis and is transported into the mitochondria for further processing in cellular respiration. It acts as a key intermediary in the breakdown of glucose.

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What does pyruvate dehydrogenase (PDH) do?

Pyruvate dehydrogenase (PDH) is an enzyme located in the mitochondrial matrix that catalyzes the conversion of pyruvate into acetyl CoA. This is a crucial step in the transition from glycolysis to the citric acid cycle.

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What is acetyl CoA?

Acetyl CoA is a molecule formed by the combination of acetyl group (from pyruvate) and coenzyme A. It plays a vital role in cellular respiration as it enters the citric acid cycle and is used to generate energy through the electron transport chain.

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What is the citric acid cycle?

The citric acid cycle (also known as the Krebs cycle or TCA cycle) is a series of biochemical reactions that produce energy in the form of ATP. It occurs in the mitochondrial matrix and involves the oxidation of acetyl CoA to carbon dioxide.

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What is the relationship between glycolysis and the citric acid cycle?

Glucose is constantly broken down into pyruvate via glycolysis, providing a continuous supply of fuel for the citric acid cycle and the electron transport chain. This ensures a steady flow of energy production within the cell.

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How does the citric acid cycle contribute to energy production?

The citric acid cycle is a series of reactions that yield NADH and FADH2. These are electron carriers that are essential for the electron transport chain, the final process of cellular respiration that produces ATP.

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What is the role of carbon dioxide in the citric acid cycle?

Carbon dioxide (CO2) is a waste byproduct released during the citric acid cycle, indicating that the fuel molecule (acetyl CoA) is being broken down to release energy. This is why we exhale carbon dioxide.

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What is the electron transport chain?

The electron transport chain is the final stage of cellular respiration, taking place on the inner mitochondrial membrane. It uses electrons from NADH and FADH2 to generate a proton gradient across the membrane, driving ATP synthesis.

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What is ATP and how is it generated?

ATP (adenosine triphosphate) is the primary energy currency of cells. It is generated through the breakdown of glucose via glycolysis, the citric acid cycle, and oxidative phosphorylation, allowing for various cellular processes.

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What is the starting material in Glycolysis?

The molecule that begins the process. It is a six-carbon sugar that serves as the primary fuel source for cellular respiration.

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What is the product of Glycolysis?

The three-carbon molecule produced at the end of glycolysis. It is a crucial intermediate that moves on to the next stage of respiration.

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What is the purpose of the first stage in Glycolysis?

The first phase of glycolysis requires an input of energy in the form of ATP. This stage prepares glucose for further breakdown.

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What happens during the second stage of Glycolysis?

This phase involves a series of reactions that produce ATP and NADH. It is where the energy harvest begins.

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What is substrate-level phosphorylation?

It refers to the transfer of a phosphate group from a molecule, such as a substrate, to ADP, generating ATP. This is a key energy-producing mechanism in glycolysis.

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What is the role of NAD+ in Glycolysis?

A molecule that is a key player in energy transfer. It carries electrons and is converted to NADH during glycolysis. This molecule is crucial for delivering energy to the electron transport chain.

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What is phosphoenolpyruvate (PEP)?

A high-energy compound that is a precursor to pyruvate. Its conversion to pyruvate produces ATP, contributing to the energy harvest of glycolysis.

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How many reactions occur in Glycolysis?

The process of converting glucose into pyruvate involves a series of 10 reactions, each catalyzed by a specific enzyme. These enzymes control the rate and direction of the reactions.

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What is pyruvate processing?

It's the process that links glycolysis to the citric acid cycle. Pyruvate is transformed into acetyl-CoA, enabling its entry into the citric acid cycle.

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Why is glycolysis important?

This process is essential for generating ATP, the primary energy currency of cells. It allows organisms to extract usable energy from glucose.

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What is anaerobic respiration?

A process that occurs in the absence of oxygen. This pathway generates ATP, but at a much lower rate than aerobic respiration.

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What is lactic acid fermentation?

It's a type of anaerobic respiration that occurs in muscle cells under conditions of low oxygen. It produces lactic acid as a byproduct, potentially causing muscle fatigue.

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Study Notes

Cellular Respiration Overview

  • Cellular respiration breaks down glucose into smaller molecules to create ATP, the cell's energy currency
  • This occurs in a series of four stages: glycolysis, pyruvate processing, the citric acid cycle, and the electron transport chain
  • The process involves oxidation-reduction reactions, transferring electrons to create energy for ATP production

Glycolysis

  • Glycolysis is the first stage of cellular respiration, occurring in the cytoplasm
  • Glucose is broken down into pyruvate (2 molecules)
  • This process uses ATP in the initial stages but results in a net gain of ATP
  • Net production = 2 ATP, 2 NADH

Pyruvate Processing

  • Pyruvate is oxidized to acetyl CoA inside the mitochondria
  • This converts pyruvate into Acetyl CoA
  • This produces 2 NADH and 2 CO2 per glucose molecule

Citric Acid Cycle

  • Acetyl CoA enters the Citric Acid Cycle (also known as the Krebs Cycle) in the mitochondrial matrix
  • It involves a series of redox reactions that produce ATP, NADH and FADH2
  • Net products per glucose are 2 ATP, 6 NADH, and 2 FADH2

Electron Transport Chain

  • The electron transport chain (ETC) is the final stage
  • Electrons from NADH and FADH2 are passed down the chain, releasing energy that is used to pump protons into the intermembrane space
  • This creates a proton gradient used by ATP synthase to generate ATP
  • Oxygen acts as the final electron acceptor, forming water
  • The ETC produces a significant number of ATP

ATP Synthase

  • The electron transport chain creates a gradient of protons (H+) between the inner and outer membrane within the mitochondria
  • ATP synthase is an enzyme that utilizes the flow of these protons to generate ATP
  • This is crucial in driving further cellular processes

NAD+ and FAD

  • NAD+ and FAD are electron carriers that move high energy electrons during the process.
  • NADH delivers electrons to the electron transport chain (ETC)
  • FADH2 also delivers electrons to the ETC, generating ATP.

What happens when oxygen is not present?

  • Cells can still produce ATP when oxygen is not available but the yield of ATP molecules is very low. The process is called anaerobic respiration or fermentation
  • In anaerobic conditions pyruvate is converted to lactate through lactate dehydrogenase (LDH)
  • In the absence of oxygen, NADH must be recycled back to NAD+ - which glycolysis needs for pyruvate processing.

Gluconeogenesis

  • When glucose levels are low, the body uses pyruvate to create glucose with the enzyme gluconeogenesis
  • The process is in the liver

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