Macronutrients and Energy Balance Quiz

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Questions and Answers

What is the normal fasting blood glucose range?

  • 90-126 mg/dL (correct)
  • 126-180 mg/dL
  • 100-150 mg/dL
  • 70-100 mg/dL

What is the main organ responsible for glucose homeostasis?

  • Pancreas
  • Liver (correct)
  • Spleen
  • Kidney

Which of the following is NOT a stage of fatty liver disease?

  • Cirrhosis
  • Hemochromatosis (correct)
  • Steatosis
  • Hepatocellular carcinoma

How does exercise affect glucose utilization?

<p>Exercise increases glucose utilization. (A)</p> Signup and view all the answers

What is the approximate glucose output of the liver per minute?

<p>150 mg/min (C)</p> Signup and view all the answers

What role does ATP play in cellular metabolism?

<p>It serves as the primary energy carrier. (B)</p> Signup and view all the answers

Which process involves the synthesis of complex molecules from simpler ones?

<p>Anabolism (A)</p> Signup and view all the answers

How does the structure of ATP facilitate its function?

<p>Its triphosphate structure allows for multiple energy-releasing reactions. (B)</p> Signup and view all the answers

What is the effect of stress on metabolism?

<p>It alters metabolic regulation, affecting both anabolic and catabolic processes. (D)</p> Signup and view all the answers

What must be restored for proper cellular function when nutrient intake is imbalanced?

<p>Homeostasis (B)</p> Signup and view all the answers

Which step in the central dogma of molecular biology is responsible for converting DNA into mRNA?

<p>Transcription (D)</p> Signup and view all the answers

What effect does allosteric regulation have on enzyme activity?

<p>It can either inhibit or excite enzyme activity. (D)</p> Signup and view all the answers

Which macronutrient provides the highest thermic effect during digestion?

<p>Proteins (B)</p> Signup and view all the answers

What is the term for the process that occurs when the body stores excess carbohydrates as glycogen?

<p>Glycogen spillover (A)</p> Signup and view all the answers

What does the first law of thermodynamics state?

<p>Total energy of a closed system is constant. (C)</p> Signup and view all the answers

Which process accurately measures the energy expenditure during physical activity?

<p>Indirect calorimetry (B)</p> Signup and view all the answers

Which factor is NOT influencing resting energy expenditure (REE)?

<p>Height (A)</p> Signup and view all the answers

What is the typical contribution of basal metabolism to daily energy expenditure?

<p>70% (A)</p> Signup and view all the answers

What interaction occurs during mRNA splicing?

<p>Exons are retained, introns are removed. (A)</p> Signup and view all the answers

Which of the following is NOT a function of micronutrients in metabolism?

<p>Providing calories for energy (C)</p> Signup and view all the answers

What does thermogenesis refer to?

<p>Heat production to maintain body temperature. (D)</p> Signup and view all the answers

What percentage of total caloric intake is typically considered energy lost in feces, urine, and respiration?

<p>10% (C)</p> Signup and view all the answers

Which nutrient is associated with the highest caloric value per gram?

<p>Fat (B)</p> Signup and view all the answers

What is the primary role of coenzymes in metabolic processes?

<p>To assist enzymes in catalyzing reactions. (B)</p> Signup and view all the answers

What is the primary product of the reaction catalyzed by citrate synthase?

<p>Citrate (C)</p> Signup and view all the answers

Which enzyme catalyzes the conversion of isocitrate to α-ketoglutarate?

<p>Isocitrate dehydrogenase (B)</p> Signup and view all the answers

What does α-ketoglutarate dehydrogenase produce from α-ketoglutarate?

<p>Succinyl-CoA (D)</p> Signup and view all the answers

What is one of the end products of the TCA cycle per acetyl-CoA molecule?

<p>3 NADH (D)</p> Signup and view all the answers

Which statement correctly describes the role of calcium in the TCA cycle?

<p>Calcium acts as an allosteric stimulator for isocitrate dehydrogenase. (A)</p> Signup and view all the answers

In electron transport, what is the role of coenzyme Q (ubiquinone)?

<p>To shuttle electrons between protein complexes (B)</p> Signup and view all the answers

Which complex in the electron transport chain is primarily responsible for pumping protons out of the mitochondrial matrix?

<p>Complex I (A)</p> Signup and view all the answers

What occurs during beta oxidation of fatty acids?

<p>Fatty acids are activated to fatty acyl-CoA. (A)</p> Signup and view all the answers

What is the final product when cytochrome C oxidase reduces oxygen?

<p>Water (B)</p> Signup and view all the answers

Which of the following is an inhibitor of the electron transport chain?

<p>Cyanide (A)</p> Signup and view all the answers

Which statement about complex II in the electron transport chain is true?

<p>Complex II exclusively oxidizes FADH2 from succinate. (C)</p> Signup and view all the answers

What type of reaction do flavoproteins primarily facilitate?

<p>Redox reactions (D)</p> Signup and view all the answers

How are protons transported across the mitochondrial membrane during oxidative phosphorylation?

<p>By coupling with the electron transport chain (C)</p> Signup and view all the answers

Which statement accurately describes the process of ATP generation during exercise?

<p>Phosphocreatine is used as a substrate for ATP generation by skeletal muscles. (B)</p> Signup and view all the answers

What happens to the energy status of a cell when the ATP:ADP ratio is high?

<p>Excess calories are converted to adipose tissue. (B)</p> Signup and view all the answers

In glycolysis, what is the first step that consumes ATP?

<p>Phosphorylation of glucose to glucose-6-phosphate. (A)</p> Signup and view all the answers

What structural feature of the inner mitochondrial membrane contributes to the energy gradient?

<p>It has a large difference in charge across its sides. (A)</p> Signup and view all the answers

Which enzyme is responsible for converting 1,3-bisphosphoglycerate to 3-phosphoglycerate in glycolysis?

<p>Phosphoglycerate kinase. (A)</p> Signup and view all the answers

During glycolysis, how many ATP molecules are produced net after processing one molecule of glucose?

<p>2 ATP (D)</p> Signup and view all the answers

Which statement describes the role of NAD+ in metabolic reactions?

<p>NAD+ accepts electrons to become NADH. (A)</p> Signup and view all the answers

What occurs during the phase of glycolysis involving the oxidation of glyceraldehyde-3-phosphate?

<p>NAD+ is reduced to NADH and phosphate is added. (C)</p> Signup and view all the answers

How does phosphocreatine contribute to ATP generation during exercise?

<p>It acts as a substrate for direct phosphorylation of ADP. (A)</p> Signup and view all the answers

Which metabolic pathway is responsible for the complete oxidation of acetyl-CoA to carbon dioxide?

<p>Krebs (TCA) cycle (D)</p> Signup and view all the answers

What is the primary product of glycolysis per glucose molecule?

<p>Pyruvate (A)</p> Signup and view all the answers

What is the role of the enzyme phosphofructokinase-1 in glycolysis?

<p>To phosphorylate fructose-6-phosphate to fructose-1,6-bisphosphate. (D)</p> Signup and view all the answers

What happens to muscle energy storage when ATP levels are low?

<p>The body initiates lipolysis and gluconeogenesis to obtain glucose. (B)</p> Signup and view all the answers

Which enzyme is responsible for removing glucose residues from glycogen?

<p>Glycogen phosphorylase (D)</p> Signup and view all the answers

What is the primary function of liver glycogen?

<p>Contribute to blood glucose levels (D)</p> Signup and view all the answers

Which of the following statements about glucose-6-phosphate is false?

<p>It is dephosphorylated in the brain before leaving the cell. (B)</p> Signup and view all the answers

How does glycogenolysis begin?

<p>Initiation by glycogen phosphorylase (B)</p> Signup and view all the answers

In which tissue does glucose-6-phosphate enter glycolysis for energy production?

<p>Muscle (A)</p> Signup and view all the answers

What is the role of pyridoxal phosphate in glycogen phosphorylase reactions?

<p>It serves as an essential cofactor. (A)</p> Signup and view all the answers

What triggers the breakdown of glycogen into glucose-1-phosphate?

<p>Contraction of muscle fibers (D)</p> Signup and view all the answers

Which of the following tissues cannot convert glucose-6-phosphate into glucose?

<p>Adipose tissue (B), Muscle (C)</p> Signup and view all the answers

What is the result of hydrolysis of glucose-6-phosphate in the liver?

<p>Glucose is released into the bloodstream. (D)</p> Signup and view all the answers

Which is NOT an outcome of glycolysis?

<p>Direct conversion of glucose to glycogen (C)</p> Signup and view all the answers

Which statement regarding glycogen is accurate?

<p>Glycogen is stored primarily in the muscle and liver. (A)</p> Signup and view all the answers

What is the function of the enzyme phosphoglucomutase?

<p>To convert glucose-1-phosphate to glucose-6-phosphate (C)</p> Signup and view all the answers

Which of the following enzymes is primarily involved in converting glucose-6-phosphate for energy utilization?

<p>Glucose 6 phosphatase (A)</p> Signup and view all the answers

Flashcards

Metabolism

The set of chemical reactions in the body that sustain life, involving enzymatic reactions affected by food intake and energy expenditure.

ATP

Adenosine triphosphate, the primary energy carrier in living cells, consisting of a ribose sugar, adenine, and three phosphate groups.

Anabolism

The process of synthesizing complex molecules from simpler ones, requiring energy (ATP).

Catabolism

The process of breaking down molecules to release energy for the body.

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Metabolic Regulation

The maintenance of anabolic and catabolic processes for homeostasis, requiring responses to nutrient intake and environmental changes.

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Blood Sugar Normal Range

The normal fasting blood glucose level is between 90-126 mg/dL.

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Hypoglycemia

A condition defined by blood glucose levels below 70 mg/dL, often causing dizziness and weakness.

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Glucose Homeostasis

The balance of glucose levels in the blood, primarily managed by the liver.

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Glucogenesis

The process of producing glucose in the liver.

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Stages of Fatty Liver Disease

Progression from normal liver to steatosis, steatohepatitis, cirrhosis, and potentially liver cancer.

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Phosphocreatine

Energy reserve in muscles used to quickly regenerate ATP.

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Substrate-level phosphorylation

Direct generation of ATP from ADP without oxygen, using specific enzymes.

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Oxidation

Process where an electron donor loses an electron.

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Reduction

Process where an electron acceptor gains an electron.

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NADH

Reduced form of Nicotinamide Adenine Dinucleotide, important in energy metabolism.

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FADH2

Reduced form of Flavin Adenine Dinucleotide, involved in the TCA cycle.

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Mitochondrial membrane permeability

Outer membrane is permeable, inner membrane is not, affecting ion transport.

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Cell energy status

Ratios of ATP:ADP and NADH:NAD+ reflect a cell's energy level.

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Glycolysis

Pathway that converts glucose into pyruvate, producing ATP.

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TCA Cycle

Metabolic pathway that oxidizes acetyl-CoA to CO2, generating NADH and ATP.

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Enzyme's role in pathways

Specific enzymes are required for each step in glycolysis and TCA cycle.

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Pyruvate oxidation

Process converting pyruvate into acetyl-CoA before entering TCA cycle.

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ATP Yield from Glycolysis

Net gain is 2 ATP and 2 NADH from one glucose molecule.

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Lipogenesis

Process of storing excess calories as fat when energy levels are high.

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Amylose

A form of glucose storage in plants that is more compact.

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Glycogen

A storage form of glucose in animals that is more soluble and easier to break down than amylose.

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Glycogen Storage Locations

Glycogen is primarily stored in the liver and muscles, contributing to energy supply.

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Glycogenolysis

The process of breaking down glycogen into glucose-1-phosphate for energy.

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Glycogen Phosphorylase

An enzyme that removes glucose units from glycogen during glycogenolysis.

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Debranching Enzyme

An enzyme that works with glycogen phosphorylase to release glucose from glycogen branches.

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Phosphoglucomutase

An enzyme that converts glucose-1-phosphate to glucose-6-phosphate for metabolism.

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Glucose-6-Phosphate

A key molecule formed from glycogenolysis that enters glycolysis or the pentose pathway.

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Liver G6P Dephosphorylation

Process of converting glucose-6-phosphate to glucose in the liver for blood glucose release.

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Glucose Contribution to Blood

Only liver glycogen can convert G6P to glucose, aiding blood glucose levels.

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Rate Limiting Step of Glycolysis

The first committed step in glycolysis, essential for trapping glucose in the cell.

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Pyruvate Fates

Pyruvate can become acetyl-CoA, lactate, or ethanol, depending on the cellular conditions.

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Triose phosphate isomerase deficiency

A lethal genetic condition causing hemolytic anemia and neurological symptoms due to glycolytic enzyme deficiency.

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Glucose Transport Mechanism

Glucose is transported based on a gradient through special transport proteins in the liver.

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Glycogen Degradation Pathway

The pathway starting from glycogen to glucose-1-phosphate, then to glucose-6-phosphate, and finally to glucose.

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Central Dogma of DNA

The process of DNA being transcribed to mRNA, which is then translated to protein.

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mRNA Processing

Modification of mRNA after transcription to prepare it for translation.

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Allosteric Regulation

Regulation of an enzyme's activity through binding of a molecule at a site other than the active site.

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Energy Balance

The relationship between energy intake and energy expenditure to maintain body weight.

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First Law of Thermodynamics

Energy cannot be created or destroyed, only transformed from one form to another.

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Macronutrients

Nutrients required in large amounts that provide energy: carbohydrates, proteins, and lipids.

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Micronutrients

Vitamins and minerals that support metabolism, required in smaller amounts.

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Thermal Effects of Food

The energy expended during digestion and processing of food.

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Glycogen Spillover

When excess carbohydrates are converted to glycogen after a high-carb meal.

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Resting Metabolic Rate (RMR)

The rate of energy expenditure while at rest, necessary for maintaining basic bodily functions.

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Thermogenesis

The process of heat production in organisms in response to temperature changes.

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Indirect Calorimetry

A method to measure energy expenditure by analyzing respiration gases.

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Adenosine Triphosphate (ATP)

A high-energy molecule that acts as the main energy currency of the cell.

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Energy Yield from Hydrolysis

The energy released when ATP is hydrolyzed, approximately 7.3 kcal per bond.

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Citrate Formation

Citrate is formed from Acetyl-COA and Oxaloacetate by citrate synthase.

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Aconitase

Aconitase converts citrate to isocitrate during isomerization.

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Isocitrate Dehydrogenase

Isocitrate dehydrogenase converts isocitrate to α-ketoglutarate, reducing NAD+ to NADH.

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Alpha-Ketoglutarate Dehydrogenase

Converts α-ketoglutarate to Succinyl-CoA, generating another NADH and releasing CO2.

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Succinyl-CoA Synthetase

Converts Succinyl-CoA to Succinate while producing GTP (or ATP).

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Succinate Dehydrogenase

Converts Succinate to Fumarate, reducing FAD to FADH2.

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Fumarase

Fumarase hydrates Fumarate to Malate.

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Malate Dehydrogenase

Converts Malate back to Oxaloacetate, generating NADH.

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Macronutrient Oxidation

Process of breaking down macronutrients like fats and carbohydrates for energy.

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Chemiosmotic Theory

Describes how the proton gradient powers ATP synthesis in mitochondria.

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Electron Transport Chain

A series of protein complexes facilitating electron transfer to create a proton gradient.

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Ubiquinone (Coenzyme Q)

A lipid-soluble carrier that shuttles electrons in the electron transport chain.

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Complex I

Transfers electrons from NADH to Ubiquinone and pumps protons into the intermembrane space.

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Complex IV

Transfers electrons to oxygen, forming water and releasing protons.

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ETC Inhibitors

Substances like Rotenone and Cyanide that disrupt the electron transport chain.

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Study Notes

Macronutrients and Energy Balance

  • Metabolism is a set of chemical reactions that sustain life, consisting of enzymatic reactions affected by food intake and energy expenditure. It varies between individuals.
  • ATP is the primary energy carrier in cells, composed of 3 phosphate groups, a ribose sugar, and adenine. Its structure allows for multiple energy-releasing reactions and movement to different cell compartments.
  • Metabolic regulation maintains homeostasis by controlling anabolic (building complex molecules) and catabolic (breaking down molecules) processes.
  • Imbalance requires restoring ATP to cells for proper function.
  • Nutrient intake (excess or inadequate) and environmental changes trigger metabolic responses.
  • Regulation occurs through short-term (hours) and long-term (weeks) mechanisms, including responses at a cellular level (transcription, translation, post-transcriptional, and post-translational steps).

Central Dogma of DNA

  • DNA is transcribed into mRNA.
  • mRNA is translated into a protein, including amino acid chains.
  • mRNA processing involves exons (coding regions) and introns (non-coding regions).

Allosteric Regulation of Enzymes

  • Allosteric regulation of enzymes occurs when a molecule binds to a site separate from the active site, either inhibiting or activating the enzyme's activity.

Energy Balance

  • Energy balance is maintained when energy intake matches energy expenditure (basal metabolism, thermal effect of food, and physical activity).
  • Basal metabolism accounts for 70% of energy expenditure.
  • Thermal effect of food accounts for 10%.
  • Physical activity accounts for 20%.

Macronutrients and Caloric Intake

  • Macronutrients are the bulk of calorie intake and include carbohydrates, proteins, and lipids.
  • Micronutrients are important for facilitators in metabolism.
  • About 90% of available energy is from ingested calories, with 10% loss through bodily functions (feces, urine, respiration).
  • The thermic effect of food is determined by nutrients and their caloric content.

Methods of Measuring Energy Expenditure

  • Indirect calorimetry measures gases produced, including resting energy expenditure (REE) and exercise energy expenditure (exercise).
  • DEXA measures lean vs fat mass.

Adenosine Triphosphate (ATP)

  • ATP consists of 3 phosphate groups linked together by high-energy bonds.
  • ATP is used to drive chemical reactions in cells during muscle contraction.

Oxidation/Reduction Reactions

  • Oxidation is the loss of electrons and reduction is the gain of electrons.
  • Electron donors are oxidized, and acceptors are reduced.
  • NAD+ is reduced to NADH and FAD to FADH2.

Mitochondria

  • Mitochondria are structures inside cells that generate energy.
  • Mitochondria have an outer and inner membrane, and a matrix with proteins for energy production.
  • They use substrate level phosphorylation to create ATP from ADP.

Cell Energy Status

  • A high ATP:ADP and NADH:NAD+ ratio indicates sufficient energy. A low ratio indicates a need for energy.

Glycolysis

  • Glycolysis is a metabolic pathway in the cytoplasm breaking down glucose to generate ATP. Glucose transforms to pyruvate, a 10-step reaction involving different enzymes. Two ATP are used initially and four are produced in the latter stages of glycolysis for a net gain of 2 ATP.

TCA Cycle (Krebs Cycle)

  • The TCA cycle oxidizes acetyl-CoA, generating NADH, FADH2 (high-energy carriers), and ATP.
  • It consists of several steps, producing molecules and energy through reactions using various enzymes.

Beta Oxidation

  • Beta oxidation is the primary pathway for fatty acid breakdown, generating acetyl-CoA, NADH, and FADH2 that enter the TCA Cycle.

Electron Transport Chain

  • The electron transport chain uses electron carriers to drive the synthesis of ATP, and creates an electrochemical gradient within the mitochondrial membrane using proton pumps.
  • Four protein complexes (I-IV) are involved. Ubiquinone and cytochrome c are mobile electron carriers.

Regulatory Reactions of the TCA Cycle

  • Regulatory mechanisms for the TCA cycle control substrate availability and enzyme activity. Changes in this cycle impact the energy production of cells.

Macronutrient Metabolism (Carbohydrates)

  • Simple carbohydrates include monosaccharides and disaccharides. Complex carbohydrates are oligosaccharides and polysaccharides.
  • Glucose metabolism is important to maintain blood sugar and the liver is a key organ.
  • Glycogens production and regulation plays a role in managing glucose levels. Glucose-6-phosphatase converts glucose-6-phosphate to glucose to release it into the bloodstream. This occurs in the liver.
  • Glycogenolysis is the breakdown of glycogen; it creates glucose 1-phosphate and glucose 6-phosphate which enters the glycolysis pathway. Different enzymatic pathways control the process and it occurs differently in various tissues.

Fates of Pyruvate

  • Pyruvate has several fates, including becoming acetyl-CoA and ultimately entering the TCA cycle or anaerobic conditions generating lactate in vertebrates/ ethanol in microorganisms.

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