Immunohistochemistry: Disease Diagnosis and Cancer Staging

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Questions and Answers

What is the main purpose of using immunohistochemistry in disease diagnosis?

  • To produce antibodies in laboratory animals
  • To identify tissue or cellular antigens (correct)
  • To detect organisms in cytologic preparations
  • To detect various epitopes in cells

Which type of antibodies in immunohistochemistry can react with various epitopes and are produced from different cells?

  • Enzyme-labeled antibodies
  • Polyclonal antibodies (correct)
  • Monoclonal antibodies
  • Immunoglobulins

What is the most common form of antibody used in immunohistochemistry?

  • IgM
  • IgG (correct)
  • IgE
  • IgA

Which enzyme is commonly used for labeling in the enzymatic method in immunohistochemistry?

<p>Alkaline phosphatase (D)</p> Signup and view all the answers

What is the main advantage of using enzyme labels in immunohistochemistry?

<p>Standard samples can be stored for a long time (D)</p> Signup and view all the answers

What is the traditional counterstain used after applying enzyme-labeled antibodies to antigens in immunohistochemistry?

<p>Hematoxylin (A)</p> Signup and view all the answers

Which chromogen produces a brown color when used with HRP enzyme in immunohistochemistry?

<p>DAB-diaminobenzidine (B)</p> Signup and view all the answers

What does the incubation process in immunohistochemistry aim to link with peroxidase?

<p>Antigen (B)</p> Signup and view all the answers

Which source primarily produces monoclonal antibodies used in immunohistochemistry?

<p>Mice (A)</p> Signup and view all the answers

In immunohistochemistry, what is the main function of fluorochrome dyes if they are used in the technique?

<p>Turn the technique into immunofluorescence (C)</p> Signup and view all the answers

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