10 Questions
What is the main purpose of using immunohistochemistry in disease diagnosis?
To identify tissue or cellular antigens
Which type of antibodies in immunohistochemistry can react with various epitopes and are produced from different cells?
Polyclonal antibodies
What is the most common form of antibody used in immunohistochemistry?
IgG
Which enzyme is commonly used for labeling in the enzymatic method in immunohistochemistry?
Alkaline phosphatase
What is the main advantage of using enzyme labels in immunohistochemistry?
Standard samples can be stored for a long time
What is the traditional counterstain used after applying enzyme-labeled antibodies to antigens in immunohistochemistry?
Hematoxylin
Which chromogen produces a brown color when used with HRP enzyme in immunohistochemistry?
DAB-diaminobenzidine
What does the incubation process in immunohistochemistry aim to link with peroxidase?
Antigen
Which source primarily produces monoclonal antibodies used in immunohistochemistry?
Mice
In immunohistochemistry, what is the main function of fluorochrome dyes if they are used in the technique?
Turn the technique into immunofluorescence
Explore the non-routine procedure of immunohistochemistry, commonly used in disease diagnosis and cancer staging. Learn about antigen-antibody reactions and its applications in identifying tissue antigens and detecting microbes in cytologic preparations.
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