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Immunohistochemistry and Cytogenetics Quiz

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Immunohistochemistry and Cytogenetics Quiz

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Questions and Answers

What is the most common application of immunostaining?

Using fluorescent labeled primary antibody

Using fluorescent labeled secondary antibody

Visualizing cellular components within cells

Identifying antigens in cells of a tissue section (correct)

What is the primary difference between Direct IHC and Indirect IHC?

Direct labeling of primary antibody with color producing material (correct)

Application in different tissue types

Use of different types of antibodies

Indirect labeling of primary antibody with color producing material

What is the protein synthesized inside the cell considered in immunohistochemistry?

Ligand

Enzyme

Antigen (correct)

Receptor

What type of microscope is used to observe tissue sections in Immunofluorescence?

<p>Fluorescent or confocal microscope</p> Signup and view all the answers

What does IHC make it possible to visualize?

<p>Distribution and localization of specific cellular components within cells</p> Signup and view all the answers

Study Notes

Immunostaining Application

Diagnosing diseases by identifying specific proteins or antigens in tissue samples

Direct vs Indirect IHC

Direct IHC: Uses a primary antibody directly conjugated to an enzyme or fluorescent tag.
Indirect IHC: Uses a primary antibody followed by a secondary antibody that is conjugated to an enzyme or fluorescent tag.

Protein Synthesized Inside Cells

Intracellular proteins are the proteins synthesized inside the cell and are the primary subject of investigation in immunohistochemistry.

Microscope Type

Fluorescence microscope is used to observe tissue sections in Immunofluorescence.

Visualization with IHC

IHC (Immunohistochemistry) allows for the visualization of the distribution and localization of specific proteins or antigens within cells and tissues.

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Description

Test your knowledge of immunohistochemistry and cytogenetics with this quiz by Dr. Mona Nasr, an associate professor of Histology and cytology. Explore the principles of immunohistochemistry, its applications, and the process of selectively identifying antigens in cells.