10 Questions
What is the main purpose of Immunohistochemistry?
Identify tissue or cellular antigens
Which type of antibodies is produced from different cells and can react with various epitopes?
Polyclonal antibodies
What is the most common form of antibody used in Immunohistochemistry?
IgG
Which enzyme is commonly used in antibody labeling during Immunohistochemistry?
HRP
What is the main advantage of using enzyme labels in Immunohistochemistry?
Standard samples can be stored for a long time
What is the traditional counterstain used after applying an enzyme-labelled antibody to the antigen?
Hematoxylin
Which chromogen produces a brown color in Immunohistochemistry?
DAB
What is the main animal source for Monoclonal Antibodies used in Immunohistochemistry?
Mice
What type of epitope does a Monoclonal Antibody react with?
One specific epitope
Why is Harris more commonly used than Mayer's form of hematoxylin for counterstaining in Immunohistochemistry?
Harris provides better color contrasting
Learn about the non-routine procedure of immunohistochemistry, used in disease diagnosis and cancer staging through antigen-antibody reactions. Discover its purposes including identifying tissue antigens, detecting organisms in cytologic preparations, and more.
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