Immunohistochemistry: Disease Diagnosis and Cancer Staging
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Questions and Answers

What is the main purpose of Immunohistochemistry?

  • Detection of blood disorders
  • Monitoring hormone levels
  • Diagnosis of infectious diseases
  • Identify tissue or cellular antigens (correct)

    • Which type of antibodies is produced from different cells and can react with various epitopes?

  • IgG antibodies
  • Enzyme-linked antibodies
  • Polyclonal antibodies (correct)
  • Monoclonal antibodies

    • What is the most common form of antibody used in Immunohistochemistry?

  • IgG (correct)
  • IgA
  • IgM
  • IgE

    • Which enzyme is commonly used in antibody labeling during Immunohistochemistry?

    <p>HRP</p> Signup and view all the answers

    What is the main advantage of using enzyme labels in Immunohistochemistry?

    <p>Standard samples can be stored for a long time</p> Signup and view all the answers

    What is the traditional counterstain used after applying an enzyme-labelled antibody to the antigen?

    <p>Hematoxylin</p> Signup and view all the answers

    Which chromogen produces a brown color in Immunohistochemistry?

    <p>DAB</p> Signup and view all the answers

    What is the main animal source for Monoclonal Antibodies used in Immunohistochemistry?

    <p>Mice</p> Signup and view all the answers

    What type of epitope does a Monoclonal Antibody react with?

    <p>One specific epitope</p> Signup and view all the answers

    Why is Harris more commonly used than Mayer's form of hematoxylin for counterstaining in Immunohistochemistry?

    <p>Harris provides better color contrasting</p> Signup and view all the answers

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