Immunohistochemistry and Cell Death Quiz
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Immunohistochemistry refers to the process of detecting protein in cells of a tissue section by exploiting the principle of antibodies binding specifically to ______ in biological tissues. It is widely used in the diagnosis of abnormal cells such as specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis).

antigens

Immunohistochemistry is also widely used in basic research to understand the distribution and localization of ______ and differentially expressed proteins in different parts of a biological tissue. While using the right antibodies to target the correct antigens and amplify the signal is important for visualization, complete preparation of the sample is critical to maintain cell morphology, tissue architecture and the antigenicity of target epitopes.

biomarkers

Paraformaldehyde is usually used with fixation. Visualizing an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an ______, such as peroxidase, that can catalyse a color-producing reaction. Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein. Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues.

enzyme

Immunohistochemistry technique and ______ Immunohistochemistry refers to the process of detecting protein in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. It is widely used in the diagnosis of abnormal cells such as specific molecular markers are characteristic of particular cellular events such as proliferation or ______ (apoptosis).

<p>cell death</p> Signup and view all the answers

Immunohistochemistry technique and cell death Immunohistochemistry refers to the process of detecting protein in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues. It is widely used in the diagnosis of abnormal cells such as specific molecular markers are characteristic of particular cellular events such as proliferation or ______.

<p>apoptosis</p> Signup and view all the answers

Paraformaldehyde is usually used with fixation. Visualizing an antibody-antigen interaction can be accomplished in a number of ways. In the most common instance, an antibody is conjugated to an ______, such as peroxidase, that can catalyse a color-producing reaction. Alternatively, the antibody can also be tagged to a fluorophore, such as fluorescein. Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues.

<p>enzyme</p> Signup and view all the answers

It is widely used in the diagnosis of abnormal cells such as specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (______). Immunohistochemistry is also widely used in basic research to understand the distribution and localization of biomarkers and differentially expressed proteins in different parts of a biological tissue.

<p>apoptosis</p> Signup and view all the answers

Immunohistochemistry refers to the process of detecting protein in cells of a tissue section by exploiting the principle of antibodies binding specifically to ______ in biological tissues. It is widely used in the diagnosis of abnormal cells such as specific molecular markers are characteristic of particular cellular events such as proliferation or cell death (apoptosis).

<p>antigens</p> Signup and view all the answers

While using the right antibodies to target the correct antigens and amplify the signal is important for visualization, complete preparation of the sample is critical to maintain cell morphology, tissue architecture and the antigenicity of target epitopes. This requires proper tissue collection, fixation and ______.

<p>sectioning</p> Signup and view all the answers

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