Identification of Enterobacteriaceae: IMViC Tests Quiz
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Questions and Answers

What does the 'I' in 'IMViC' stand for?

  • Inhibition
  • Isolation
  • Indole (correct)
  • Identification
  • Which reagent is used to detect indole production in the Indole Test?

  • Kovac's or Ehrlich's reagent (correct)
  • Benedict's reagent
  • Tollen's reagent
  • Fehling's solution
  • What color indicates a positive result in the Indole Test?

  • Blue
  • Red-violet (correct)
  • Green
  • Yellow
  • Which bacteria are Indole-positive?

    <p>E. coli, P. vulgaris, Shigella spp., K. oxytoca</p> Signup and view all the answers

    Which two tests are performed using a single broth as part of the IMViC tests?

    <p>Methyl red and Voges-Proskauer tests</p> Signup and view all the answers

    What does a positive MR test indicate about the organism being tested?

    <p>It produces acid from glucose fermentation</p> Signup and view all the answers

    Which test is used to detect the acidic end product of glucose metabolism?

    <p>Methyl Red (MR) test</p> Signup and view all the answers

    Which bacteria produce a positive Methyl Red (MR) test result?

    <p>Enterobacter aerogenes and Klebsiella pneumoniae</p> Signup and view all the answers

    Which test is used to distinguish between members of the Enterobacteriaceae family based on citrate utilization?

    <p>Citrate utilization Test</p> Signup and view all the answers

    What color change indicates a positive Voges–Proskauer (VP) Test result?

    <p>Yellow to red</p> Signup and view all the answers

    Which bacteria are Citrate negative according to the Citrate utilization Test?

    <p>Escherichia coli and Shigella spp</p> Signup and view all the answers

    What does the Urease Test detect the ability of an organism to produce?

    <p>Carbon dioxide and ammonia</p> Signup and view all the answers

    Which organism is Urease negative according to the Urease Test?

    <p>E.coli</p> Signup and view all the answers

    Which enzyme does the Oxidase test determine the presence of?

    <p>Cytochrome oxidase enzyme</p> Signup and view all the answers

    Which family of bacteria are typically Oxidase negative according to the Oxidase test?

    <p>Enterobacteriaceae</p> Signup and view all the answers

    'Members of Enterobacteriaceae family are catalase positive' refers to the ability of these bacteria to:

    <p>Break down hydrogen peroxide into water and oxygen</p> Signup and view all the answers

    Study Notes

    IMViC Tests

    • The 'I' in 'IMViC' stands for Indole
    • Kovac's reagent is used to detect indole production in the Indole test
    • A red layer indicates a positive result in the Indole Test
    • Escherichia coli is an example of an Indole-positive bacteria
    • The MR-VP tests are performed using a single broth
    • A positive Methyl Red (MR) test indicates the organism produces mixed acids as a result of glucose metabolism
    • The Methyl Red (MR) test is used to detect the acidic end product of glucose metabolism
    • Escherichia coli and Salmonella are bacteria that produce a positive Methyl Red (MR) test result
    • The Citrate utilization Test is used to distinguish between members of the Enterobacteriaceae family based on citrate utilization
    • A blue color change indicates a positive Voges–Proskauer (VP) Test result
    • Escherichia coli and Salmonella are examples of Citrate negative bacteria according to the Citrate utilization Test
    • The Urease Test detects the ability of an organism to produce urease
    • Proteus mirabilis is a Urease negative organism according to the Urease Test
    • The Oxidase test determines the presence of cytochrome c oxidase
    • The Enterobacteriaceae family are typically Oxidase negative according to the Oxidase test
    • 'Members of Enterobacteriaceae family are catalase positive' refers to the ability of these bacteria to break down hydrogen peroxide into water and oxygen.

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    Description

    Test your knowledge of the IMViC tests used for the identification of members of the Enterobacteriaceae family, including the Indole, Methyl red, Voges-Proskauer, and Citrate tests. This quiz covers the mediums, reagents, and procedures involved in these tests.

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