Identification of Enterobacteriaceae: IMViC Tests Quiz

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16 Questions

What does the 'I' in 'IMViC' stand for?

Indole

Which reagent is used to detect indole production in the Indole Test?

Kovac's or Ehrlich's reagent

What color indicates a positive result in the Indole Test?

Red-violet

Which bacteria are Indole-positive?

E. coli, P. vulgaris, Shigella spp., K. oxytoca

Which two tests are performed using a single broth as part of the IMViC tests?

Methyl red and Voges-Proskauer tests

What does a positive MR test indicate about the organism being tested?

It produces acid from glucose fermentation

Which test is used to detect the acidic end product of glucose metabolism?

Methyl Red (MR) test

Which bacteria produce a positive Methyl Red (MR) test result?

Enterobacter aerogenes and Klebsiella pneumoniae

Which test is used to distinguish between members of the Enterobacteriaceae family based on citrate utilization?

Citrate utilization Test

What color change indicates a positive Voges–Proskauer (VP) Test result?

Yellow to red

Which bacteria are Citrate negative according to the Citrate utilization Test?

Escherichia coli and Shigella spp

What does the Urease Test detect the ability of an organism to produce?

Carbon dioxide and ammonia

Which organism is Urease negative according to the Urease Test?

E.coli

Which enzyme does the Oxidase test determine the presence of?

Cytochrome oxidase enzyme

Which family of bacteria are typically Oxidase negative according to the Oxidase test?

Enterobacteriaceae

'Members of Enterobacteriaceae family are catalase positive' refers to the ability of these bacteria to:

Break down hydrogen peroxide into water and oxygen

Test your knowledge of the IMViC tests used for the identification of members of the Enterobacteriaceae family, including the Indole, Methyl red, Voges-Proskauer, and Citrate tests. This quiz covers the mediums, reagents, and procedures involved in these tests.

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