Microbiology Quiz: Bacteriology

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Questions and Answers

Which bacterium is characterized by a 'tackhead' appearance due to a swollen terminal spore?

  • Clostridium botulinum
  • Yersinia
  • Bacillus anthracis
  • Clostridium tetani (correct)

What is the characteristic appearance of Corynebacterium diptheriae under a microscope?

  • Pleomorphic, resembling a 'Chinese character' (correct)
  • Rod-shaped, gram-negative
  • Spiral-shaped, gram-positive
  • Lancet-shaped, catalase negative

What is the selective medium for Pseudomonas aeruginosa?

  • Mueller Hinton agar
  • Cetrimide agar (correct)
  • Chocolate agar
  • MacConkey agar

What is the purpose of the Elek test?

<p>To confirm Corynebacterium diptheriae (A)</p> Signup and view all the answers

What is the characteristic appearance of Pseudomonas aeruginosa on MacConkey agar?

<p>Grapefruit-like colonies (C)</p> Signup and view all the answers

What is the purpose of adding zinc dust to a nitrate reduction test?

<p>To confirm a negative result (A)</p> Signup and view all the answers

Which of the following organisms is a gram-positive bacterium?

<p>C. perfringens (D)</p> Signup and view all the answers

What is the primary component of the cell wall in gram-positive bacteria that is stained by Gram stain?

<p>Peptidoglycan (B)</p> Signup and view all the answers

Which of the following bacteria is known for its ability to grow in slightly acidic pH?

<p>S. pneumoniae (B)</p> Signup and view all the answers

What is the term for the ability of bacteria to float in a zinc sulfate solution?

<p>Flotation (A)</p> Signup and view all the answers

Which of the following bacteria is known for its characteristic 'fishy' odor?

<p>G. vaginalis (B)</p> Signup and view all the answers

Which automated system is used for susceptibility testing?

<p>Vitek (A)</p> Signup and view all the answers

What is the purpose of the ONPG test in laboratory diagnosis?

<p>To determine if an organism is a late lactose fermenter or a true non-lactose fermenter (A)</p> Signup and view all the answers

What is the morphology of Leptospira?

<p>Spirochete with tight coils and hooked ends (D)</p> Signup and view all the answers

What is the purpose of the Citrate Utilization Test (Simmon's Citrate Test)?

<p>To identify bacteria based on their ability to utilize citrate as a source of carbon (D)</p> Signup and view all the answers

Which of the following is NOT a selective media?

<p>Mueller-Hinton Agar (C)</p> Signup and view all the answers

What is the function of Safranin in Gram staining?

<p>Secondary stain (counterstain) (A)</p> Signup and view all the answers

Which of the following bacteria is NOT associated with food poisoning?

<p>Neisseria meningitidis (D)</p> Signup and view all the answers

What type of toxin is produced by E. coli O157:H7?

<p>Shiga toxin (B)</p> Signup and view all the answers

Which medium is commonly used to detect H2S production?

<p>Triple sugar iron agar (D)</p> Signup and view all the answers

What is the characteristic of Mycobacterium tuberculosis?

<p>Acid-fast (D)</p> Signup and view all the answers

Which disease is commonly associated with pigeon droppings?

<p>Cryptococcosis (B)</p> Signup and view all the answers

What is the best method for detecting Plasmodium species?

<p>Both thick and thin smears (B)</p> Signup and view all the answers

Which of the following bacteria is mismatched with its corresponding disease?

<p>Yersinia enterocolitica - diptheriae (A)</p> Signup and view all the answers

What is the characteristic growth time for rapid growing mycobacteria?

<p>&lt;7 days (B)</p> Signup and view all the answers

Which of the following is a mismatched characteristic of bacterial structures?

<p>Endospore - reproduction (C)</p> Signup and view all the answers

What is the characteristic of the helminth egg Trichuris trichiura?

<p>Bile stained, with bipolar plugs (D)</p> Signup and view all the answers

What is the characteristic of transmission of Salmonella subgroup III?

<p>Transmission through pet snakes, lizards, and fish (B)</p> Signup and view all the answers

Which bacterium is known to cause atypical pneumonia?

<p>Mycoplasma pneumoniae (A)</p> Signup and view all the answers

Which of the following gram-negative bacilli is oxidase positive and has a characteristic grape-like odor?

<p>Pseudomonas aeruginosa (D)</p> Signup and view all the answers

What is the causative agent of anthrax?

<p>Bacillus anthracis (C)</p> Signup and view all the answers

The Quellung reaction is used for serotyping which bacterium?

<p>Streptococcus pneumoniae (B)</p> Signup and view all the answers

Which enteric pathogen produces a black precipitate on Hektoen enteric agar?

<p>Salmonella typhi (C)</p> Signup and view all the answers

Which bacterium produces a red pigment?

<p>Serratia marcescens (B)</p> Signup and view all the answers

What is the best test for the presumptive identification of S.pneumoniae?

<p>Optochin susceptibility test (A)</p> Signup and view all the answers

Which organism is commonly isolated from urethral discharge and has a fried egg appearance on culture?

<p>Mycoplasma hominis (A)</p> Signup and view all the answers

What is the most common parasite found in the Philippines?

<p>Ascaris lumbricoides (D)</p> Signup and view all the answers

What is the purpose of using Oxacillin in susceptibility testing?

<p>To detect MRSA (A)</p> Signup and view all the answers

Which of the following bacteria is an obligate intracellular pathogen?

<p>Chlamydia trachomatis (B)</p> Signup and view all the answers

What is the appearance of Cryptococcus neoformans on an India ink preparation?

<p>Budding yeast with a large capsule (D)</p> Signup and view all the answers

Which of the following bacteria produces an acid-fast toxin?

<p>Mycobacterium tuberculosis (D)</p> Signup and view all the answers

What is the causative agent of whooping cough?

<p>Bordetella pertussis (A)</p> Signup and view all the answers

What is the purpose of the Ziehl-Neelsen stain?

<p>To identify acid-fast bacteria (C)</p> Signup and view all the answers

Which of the following bacteria is commonly associated with dental caries?

<p>Streptococcus mutans (A)</p> Signup and view all the answers

What is the primary medium for the cultivation of Mycobacterium tuberculosis?

<p>Lowenstein-Jensen medium (A)</p> Signup and view all the answers

Which bacterium is known to produce a blue-green pigment?

<p>Pseudomonas aeruginosa (D)</p> Signup and view all the answers

What is the causative agent of syphilis?

<p>Treponema pallidum (B)</p> Signup and view all the answers

Which bacterium is commonly associated with biofilm formation on medical devices?

<p>Staphylococcus epidermidis (C)</p> Signup and view all the answers

What is the novobiocin-resistant coagulase-negative staphylococcus?

<p>Staphylococcus saprophyticus (C)</p> Signup and view all the answers

Which bacterium exhibits swarming motility on agar plates?

<p>Proteus mirabilis (A)</p> Signup and view all the answers

What is the characteristic growth pattern of Mycobacterium tuberculosis?

<p>Slow growth (A)</p> Signup and view all the answers

Which of the following bacteria is known to cause atypical pneumonia?

<p>Mycoplasma pneumoniae (A)</p> Signup and view all the answers

What is the primary mode of transmission for Salmonella subgroup III?

<p>Contaminated food and water (C)</p> Signup and view all the answers

Which of the following bacteria is characterized by a metachromatic granule?

<p>C. diptheriae (C)</p> Signup and view all the answers

What is the primary function of Mueller Hinton agar?

<p>Antimicrobial susceptibility testing (D)</p> Signup and view all the answers

What is the result of a positive nitrate reduction test after addition of zinc dust?

<p>Red color (A)</p> Signup and view all the answers

Which of the following bacteria is characterized by a 'grape-fruit' appearance on MacConkey agar?

<p>P. aeruginosa (D)</p> Signup and view all the answers

Which of the following stains is used to detect metachromatic granules in C. diptheriae?

<p>Methylene blue (A)</p> Signup and view all the answers

Which of the following bacteria is responsible for epidemic gastroenteritis?

<p>Norwalk virus (D)</p> Signup and view all the answers

What type of bacteria grows on MacConkey agar EXCEPT:

<p>Legionella (A)</p> Signup and view all the answers

Which of the following helminth eggs do not float in zinc sulfate solution:

<p>Paragonimus spp and D. latum (D)</p> Signup and view all the answers

Which automated system is used for susceptibility testing:

<p>Vitek (C)</p> Signup and view all the answers

Which bacteria has branched hyphae and is transmitted through inhalation of soil/dust:

<p>Nocardia (C)</p> Signup and view all the answers

Which bacterium can be characterized by a 'grape-fruit' appearance in MacConkey agar?

<p>Pseudomonas aeruginosa (A)</p> Signup and view all the answers

What is the purpose of the Elek test in bacteriology?

<p>To identify Corynebacterium diptheriae (A)</p> Signup and view all the answers

Which of the following bacteria is characterized by a 'tackhead' appearance due to a swollen terminal spore?

<p>Clostridium tetani (A)</p> Signup and view all the answers

What is the primary medium for antimicrobial susceptibility testing?

<p>Mueller Hinton agar (D)</p> Signup and view all the answers

What is the result of adding zinc dust to a negative nitrate reduction test?

<p>Development of a red color (D)</p> Signup and view all the answers

Which bacterium produces Shiga toxin?

<p>E. coli O157:H7 (A)</p> Signup and view all the answers

Which disease is commonly associated with pigeon droppings?

<p>Cryptococcosis (B)</p> Signup and view all the answers

Which Plasmodium species is best detected in?

<p>Both thick and thin smears (D)</p> Signup and view all the answers

Which bacterium is responsible for swimmer's ear?

<p>Pseudomonas aeruginosa (C)</p> Signup and view all the answers

Which organism has a double zone of hemolysis on blood agar?

<p>Clostridium perfringens (B)</p> Signup and view all the answers

Which of the following bacteria exhibits tumbling motility at room temperature?

<p>Listeria monocytogenes (B)</p> Signup and view all the answers

What is the primary use of chocolate agar in microbiology?

<p>Isolation of Neisseria and Haemophilus (B)</p> Signup and view all the answers

Which of the following tests is used to differentiate between group D streptococcus and enterococcus?

<p>PYR (D)</p> Signup and view all the answers

Which of the following bacteria is not an enteric pathogen?

<p>Bacillus cereus (A)</p> Signup and view all the answers

What is the primary purpose of a cold enrichment technique in microbiology?

<p>Isolation of Listeria monocytogenes (C)</p> Signup and view all the answers

Which of the following bacteria is associated with melioidosis, a disease also known as the 'Vietnamese time bomb'?

<p>B. pseudomallei (D)</p> Signup and view all the answers

Which of the following bacterial colonies is characterized by a green metallic sheen on EMB agar?

<p>Escherichia coli (B)</p> Signup and view all the answers

What is the primary function of Safranin in Gram staining?

<p>Counterstain (A)</p> Signup and view all the answers

Which of the following bacteria is NOT associated with food poisoning?

<p>N. meningitides (C)</p> Signup and view all the answers

Which of the following bacteria produces a characteristic 'arrowhead' shape on agar plates?

<p>CAMP (A)</p> Signup and view all the answers

Which bacterium is responsible for the production of a yellow-green pigment?

<p>Pseudomonas aeruginosa (B)</p> Signup and view all the answers

Which of the following bacteria is commonly associated with the transmission of diseases through contaminated water?

<p>Leptospira (A)</p> Signup and view all the answers

Which bacterium is known to produce a toxin that causes a severe bloody diarrhea?

<p>E. coli O157:H7 (B)</p> Signup and view all the answers

Which of the following bacteria is capable of metabolizing citrate as a sole source of carbon?

<p>Salmonella subgroup III (B)</p> Signup and view all the answers

Which bacterium is known to produce a beta-lactamase enzyme that makes it resistant to penicillin?

<p>Staphylococcus aureus (C)</p> Signup and view all the answers

Which bacterium is characterized by producing a grape-like odor and is oxidase positive?

<p>Pseudomonas aeruginosa (D)</p> Signup and view all the answers

What is the causative agent of anthrax?

<p>Bacillus anthracis (C)</p> Signup and view all the answers

Which bacterium is responsible for 'rice-water stool'?

<p>Vibrio cholerae (C)</p> Signup and view all the answers

Which bacterium produces a black precipitate on Hektoen enteric agar?

<p>Salmonella typhi (D)</p> Signup and view all the answers

What is the purpose of the Quellung reaction?

<p>To serotype Streptococcus pneumoniae (B)</p> Signup and view all the answers

Which bacterium is urease positive?

<p>Proteus mirabilis (C)</p> Signup and view all the answers

What is the causative agent of atypical pneumonia?

<p>Mycoplasma pneumoniae (D)</p> Signup and view all the answers

Which bacterium is known to cause 'fishy' odor?

<p>Proteus mirabilis (D)</p> Signup and view all the answers

What is the characteristic of Salmonella typhi?

<p>Produces a black precipitate on Hektoen enteric agar (D)</p> Signup and view all the answers

Which bacterium is associated with food poisoning?

<p>All of the above (D)</p> Signup and view all the answers

Which of the following bacteria is primarily transmitted through contaminated food and water?

<p>Escherichia coli (A)</p> Signup and view all the answers

What is the characteristic morphology of the helminth egg Trichuris trichiura?

<p>Oval-shaped with bipolar plugs (A)</p> Signup and view all the answers

Which of the following bacteria exhibits tumbling motility at room temperature?

<p>Listeria monocytogenes (D)</p> Signup and view all the answers

Which of the following bacteria produces a Shiga toxin?

<p>Escherichia coli (B)</p> Signup and view all the answers

Which of the following media is primarily used for the isolation of Neisseria and Haemophilus?

<p>Chocolate agar (B)</p> Signup and view all the answers

Flashcards

Clostridium tetani

Has a swollen terminal spore, resembling a "tackhead".

Corynebacterium diptheriae

Gram-positive, highly pleomorphic, and resembles "Chinese characters".

Streptococcus pneumoniae

Gram-positive, lancet shaped, catalase negative, susceptible to optochin.

Cetrimide agar

Selective for Pseudomonas aeruginosa.

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Methylene blue

Stains metachromatic granules, used in Corynebacterium diptheriae.

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Mueller Hinton agar

Used for antimicrobial susceptibility testing.

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Elek test

Test used to identify Corynebacterium diptheriae.

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Bile esculin test

Test used to identify Enterococcus.

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Nitrate reduction test

Test used to identify bacteria. Negative reaction confirmed by red color after adding zinc dust.

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Streptococcus pneumoniae Virulence

Capsule made for virulent strains.

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E. coli O157:H7

Produces Shiga toxin.

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Pseudomonas aeruginosa

Causes swimmer's ear.

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Borrelia burgdorferi

Spirochete that causes Lyme disease.

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Cryptococcosis

Disease commonly associated with pigeon droppings.

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Histoplasmosis

Disease associated with inhalation of soil/dust.

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Trichuris trichiura

Has a bile-stained egg with bipolar plugs.

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Plasmodium species

Best detected in both thick and thin blood smears.

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Clostridium perfringens

Has a double zone of hemolysis on blood agar.

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Nocardia

Has branched hyphae transmitted through inhalation of soil/dust.

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Peptidoglycan

Component in Gram-positive organisms that stains with Gram-stain.

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Chocolate agar

Primarily used for the isolation of Neisseria and Haemophilus.

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Bacillus cereus

Not an enteric pathogen.

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Listeria monocytogenes

Exhibits tumbling motility at room temperature.

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Cold enrichment technique

Used to enhance the isolation of Listeria monocytogenes.

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E. coli on EMB agar

Results in a green metallic sheen on EMB agar, likely Escherichia coli.

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Staphylococcus aureus

Coagulase-positive, Gram-positive cocci arranged in clusters.

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Staphylococcus saprophyticus

Coagulase-negative staphylococcus that is novobiocin resistant.

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Staphylococcus epidermidis

Commonly associated with biofilm formation on medical devices.

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Streptococcus mutans

Responsible for the formation of dental caries.

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