Histopathology Laboratory Techniques
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Questions and Answers

What is the primary purpose of dehydration in histology and cytology?

  • To increase the viscosity of the processing solvent
  • To preserve the tissue's natural shape and structure
  • To remove excess histological stains from the tissue
  • To remove unbound water and fixatives from tissue components (correct)

    • What is the consequence of excessive dehydration in tissue preparation?

  • Enhanced tissue preservation
  • Cell distortion and hardening (correct)
  • Tissue softening and receptivity to infiltration
  • Improved staining quality

    • Which of the following is NOT a common dehydrating fluid in histology and cytology?

  • Ethanol
  • Methanol
  • Isopropanol
  • Glycerol (correct)

    • What is the primary function of a clearing agent in histology and cytology?

    <p>To act as an intermediary between dehydration and infiltration solutions</p> Signup and view all the answers

    What is the purpose of loading the tissue cassettes into the chamber of the tissue processor?

    <p>To automate the dehydration and infiltration process</p> Signup and view all the answers

    What is a critical precaution to take during the embedding process in histology and cytology?

    <p>To avoid cross-contamination of tissues</p> Signup and view all the answers

    What is a characteristic of celloidin that makes it useful in neurological specimens?

    <p>Good elasticity</p> Signup and view all the answers

    Why is gelatin not commonly used for double embedding?

    <p>It has a low melting point</p> Signup and view all the answers

    What is the purpose of infiltration in the embedding process?

    <p>To support the tissue, allowing thin sections to be cut</p> Signup and view all the answers

    What is a disadvantage of using paraffin wax as an embedding medium?

    <p>It causes tissue shrinkage</p> Signup and view all the answers

    What is the primary function of a microtome?

    <p>To cut biological specimens into very thin sections</p> Signup and view all the answers

    Why is it important to ensure sufficient infiltration of the embedding medium?

    <p>To prevent the wax from hardening properly</p> Signup and view all the answers

    What is the purpose of using xylene in tissue processing?

    <p>To remove water from the tissue, especially in fatty tissue</p> Signup and view all the answers

    What is the primary advantage of using paraffin wax in tissue processing?

    <p>It is an excellent lipid solvent</p> Signup and view all the answers

    What is the primary difference between paraffin wax and ester wax?

    <p>Ester wax has a higher melting point than paraffin wax</p> Signup and view all the answers

    What is the purpose of using additives in paraffin wax?

    <p>To increase the hardness of paraffin wax</p> Signup and view all the answers

    What is the purpose of using agar in double embedding with paraffin wax?

    <p>To improve the adhesion of paraffin wax to the tissue</p> Signup and view all the answers

    What is the primary factor that determines the optimal processing schedule in tissue processing?

    <p>All of the above</p> Signup and view all the answers

    What is the purpose of having a spill kit available in a laboratory?

    <p>To clean up chemical spills</p> Signup and view all the answers

    How do cells adhere to the ThinPrep Microscope Slide?

    <p>Through surface tension and air pressure</p> Signup and view all the answers

    What is the recommended handling procedure for histology specimens received in formalin?

    <p>Handling on a Ventilated Specimen Table</p> Signup and view all the answers

    What is the importance of adequate ventilation in a laboratory?

    <p>To prevent exposure to hazardous chemicals</p> Signup and view all the answers

    What is the purpose of a Filter in liquid-based cytology?

    <p>To separate debris and disperse mucus</p> Signup and view all the answers

    Why should eye wash and shower facilities be available in a laboratory?

    <p>To respond to accidents involving chemicals</p> Signup and view all the answers

    What is the typical thickness of sections cut using an ultramicrotome for transmission electron microscopy?

    <p>40-100 nanomicron</p> Signup and view all the answers

    What is the purpose of the boat in the ultramicrotomy process?

    <p>To allow the section to float on water</p> Signup and view all the answers

    What is the advantage of Profile B knives in section cutting?

    <p>They can be sharpened to a very sharp edge</p> Signup and view all the answers

    What is the function of Eosin in histology?

    <p>It is an acidic and negatively charged dye used as a counterstain</p> Signup and view all the answers

    What is an advantage of using Profile D knives?

    <p>They are used to cut hard tissue such as decalcified bone</p> Signup and view all the answers

    What is an advantage of rapid production of sections for intraoperative diagnosis?

    <p>It allows for faster diagnosis and treatment</p> Signup and view all the answers

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