Histopathology Laboratory Techniques

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Questions and Answers

What is the primary purpose of dehydration in histology and cytology?

To increase the viscosity of the processing solvent
To preserve the tissue's natural shape and structure
To remove excess histological stains from the tissue
To remove unbound water and fixatives from tissue components (correct)

What is the consequence of excessive dehydration in tissue preparation?

Enhanced tissue preservation
Cell distortion and hardening (correct)
Tissue softening and receptivity to infiltration
Improved staining quality

Which of the following is NOT a common dehydrating fluid in histology and cytology?

Ethanol
Methanol
Isopropanol
Glycerol (correct)

What is the primary function of a clearing agent in histology and cytology?

To act as an intermediary between dehydration and infiltration solutions (C) Signup and view all the answers

What is the purpose of loading the tissue cassettes into the chamber of the tissue processor?

To automate the dehydration and infiltration process (A) Signup and view all the answers

What is a critical precaution to take during the embedding process in histology and cytology?

To avoid cross-contamination of tissues (B) Signup and view all the answers

What is a characteristic of celloidin that makes it useful in neurological specimens?

Good elasticity (B) Signup and view all the answers

Why is gelatin not commonly used for double embedding?

It has a low melting point (D) Signup and view all the answers

What is the purpose of infiltration in the embedding process?

To support the tissue, allowing thin sections to be cut (B) Signup and view all the answers

What is a disadvantage of using paraffin wax as an embedding medium?

It causes tissue shrinkage (B) Signup and view all the answers

What is the primary function of a microtome?

To cut biological specimens into very thin sections (D) Signup and view all the answers

Why is it important to ensure sufficient infiltration of the embedding medium?

To prevent the wax from hardening properly (A) Signup and view all the answers

What is the purpose of using xylene in tissue processing?

To remove water from the tissue, especially in fatty tissue (C) Signup and view all the answers

What is the primary advantage of using paraffin wax in tissue processing?

It is an excellent lipid solvent (D) Signup and view all the answers

What is the primary difference between paraffin wax and ester wax?

Ester wax has a higher melting point than paraffin wax (D) Signup and view all the answers

What is the purpose of using additives in paraffin wax?

To increase the hardness of paraffin wax (A) Signup and view all the answers

What is the purpose of using agar in double embedding with paraffin wax?

To improve the adhesion of paraffin wax to the tissue (B) Signup and view all the answers

What is the primary factor that determines the optimal processing schedule in tissue processing?

All of the above (D) Signup and view all the answers

What is the purpose of having a spill kit available in a laboratory?

To clean up chemical spills (C) Signup and view all the answers

How do cells adhere to the ThinPrep Microscope Slide?

Through surface tension and air pressure (B) Signup and view all the answers

What is the recommended handling procedure for histology specimens received in formalin?

Handling on a Ventilated Specimen Table (A) Signup and view all the answers

What is the importance of adequate ventilation in a laboratory?

To prevent exposure to hazardous chemicals (D) Signup and view all the answers

What is the purpose of a Filter in liquid-based cytology?

To separate debris and disperse mucus (C) Signup and view all the answers

Why should eye wash and shower facilities be available in a laboratory?

To respond to accidents involving chemicals (B) Signup and view all the answers

What is the typical thickness of sections cut using an ultramicrotome for transmission electron microscopy?

40-100 nanomicron (B) Signup and view all the answers

What is the purpose of the boat in the ultramicrotomy process?

To allow the section to float on water (A) Signup and view all the answers

What is the advantage of Profile B knives in section cutting?

They can be sharpened to a very sharp edge (D) Signup and view all the answers

What is the function of Eosin in histology?

It is an acidic and negatively charged dye used as a counterstain (B) Signup and view all the answers

What is an advantage of using Profile D knives?

They are used to cut hard tissue such as decalcified bone (D) Signup and view all the answers

What is an advantage of rapid production of sections for intraoperative diagnosis?

It allows for faster diagnosis and treatment (A) Signup and view all the answers

Flashcards

Purpose of Dehydration (Histology)

Removes unbound water and fixatives from tissue components for better processing.

Excessive Dehydration

Leads to tissue distortion and hardening during tissue preparation.