30 Questions
What is the primary purpose of dehydration in histology and cytology?
To remove unbound water and fixatives from tissue components
What is the consequence of excessive dehydration in tissue preparation?
Cell distortion and hardening
Which of the following is NOT a common dehydrating fluid in histology and cytology?
Glycerol
What is the primary function of a clearing agent in histology and cytology?
To act as an intermediary between dehydration and infiltration solutions
What is the purpose of loading the tissue cassettes into the chamber of the tissue processor?
To automate the dehydration and infiltration process
What is a critical precaution to take during the embedding process in histology and cytology?
To avoid cross-contamination of tissues
What is a characteristic of celloidin that makes it useful in neurological specimens?
Good elasticity
Why is gelatin not commonly used for double embedding?
It has a low melting point
What is the purpose of infiltration in the embedding process?
To support the tissue, allowing thin sections to be cut
What is a disadvantage of using paraffin wax as an embedding medium?
It causes tissue shrinkage
What is the primary function of a microtome?
To cut biological specimens into very thin sections
Why is it important to ensure sufficient infiltration of the embedding medium?
To prevent the wax from hardening properly
What is the purpose of using xylene in tissue processing?
To remove water from the tissue, especially in fatty tissue
What is the primary advantage of using paraffin wax in tissue processing?
It is an excellent lipid solvent
What is the primary difference between paraffin wax and ester wax?
Ester wax has a higher melting point than paraffin wax
What is the purpose of using additives in paraffin wax?
To increase the hardness of paraffin wax
What is the purpose of using agar in double embedding with paraffin wax?
To improve the adhesion of paraffin wax to the tissue
What is the primary factor that determines the optimal processing schedule in tissue processing?
All of the above
What is the purpose of having a spill kit available in a laboratory?
To clean up chemical spills
How do cells adhere to the ThinPrep Microscope Slide?
Through surface tension and air pressure
What is the recommended handling procedure for histology specimens received in formalin?
Handling on a Ventilated Specimen Table
What is the importance of adequate ventilation in a laboratory?
To prevent exposure to hazardous chemicals
What is the purpose of a Filter in liquid-based cytology?
To separate debris and disperse mucus
Why should eye wash and shower facilities be available in a laboratory?
To respond to accidents involving chemicals
What is the typical thickness of sections cut using an ultramicrotome for transmission electron microscopy?
40-100 nanomicron
What is the purpose of the boat in the ultramicrotomy process?
To allow the section to float on water
What is the advantage of Profile B knives in section cutting?
They can be sharpened to a very sharp edge
What is the function of Eosin in histology?
It is an acidic and negatively charged dye used as a counterstain
What is an advantage of using Profile D knives?
They are used to cut hard tissue such as decalcified bone
What is an advantage of rapid production of sections for intraoperative diagnosis?
It allows for faster diagnosis and treatment
Test your knowledge of histopathology laboratory techniques, including specimen reception, slide preparation, and dehydration methods. Learn about the importance of proper techniques to avoid cell distortion and ensure accurate results.
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