Histopathology and Cytology: Faults in Section Cutting and Tissue Processing

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11 Questions

What is a possible reason for the failure to form ribbons in G sections?

The surfaces and edges of the block are not parallel

What can be done to fix compressed, wrinkled, or jammed sections?

Resharpen the knife and cool the block on ice water

Why might the knife edge need to be cleaned?

The knife edge is coated with paraffin

What can be done to prevent the formation of compressed, wrinkled, or jammed sections?

Reduce the tilt of the knife

Why might the paraffin block need to be cooled?

The block is too warm

What is the reason for sections expanding or disintegrating on the water bath?

Poor impregnation of tissue

What is the remedy for a dull knife blade?

Hone and strop the knife

Why do sections roll into a coil instead of remaining flat on the knife edge?

Blade is dull

What can cause sections to roll up on cutting and adhere to the knife edge?

Blade is blunt

Why do sections become grooved, scored, smeared, and deformed?

Blade is dull

What can cause the ribbon to be curved, crooked, or uneven instead of straight?

Blunt knife edge

Identify and rectify common errors that occur during section cutting and tissue processing in histopathology and cytology, including issues with section thickness, temperature, and blade sharpness.

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