11 Questions
What is a possible reason for the failure to form ribbons in G sections?
The surfaces and edges of the block are not parallel
What can be done to fix compressed, wrinkled, or jammed sections?
Resharpen the knife and cool the block on ice water
Why might the knife edge need to be cleaned?
The knife edge is coated with paraffin
What can be done to prevent the formation of compressed, wrinkled, or jammed sections?
Reduce the tilt of the knife
Why might the paraffin block need to be cooled?
The block is too warm
What is the reason for sections expanding or disintegrating on the water bath?
Poor impregnation of tissue
What is the remedy for a dull knife blade?
Hone and strop the knife
Why do sections roll into a coil instead of remaining flat on the knife edge?
Blade is dull
What can cause sections to roll up on cutting and adhere to the knife edge?
Blade is blunt
Why do sections become grooved, scored, smeared, and deformed?
Blade is dull
What can cause the ribbon to be curved, crooked, or uneven instead of straight?
Blunt knife edge
Identify and rectify common errors that occur during section cutting and tissue processing in histopathology and cytology, including issues with section thickness, temperature, and blade sharpness.
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