Histopathology and Cytology: Faults in Section Cutting and Tissue Processing
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Questions and Answers

What is a possible reason for the failure to form ribbons in G sections?

  • The microtome set screw is loose
  • The surfaces and edges of the block are not parallel (correct)
  • The paraffin wax is too soft
  • The knife is blunt or dull

    • What can be done to fix compressed, wrinkled, or jammed sections?

  • Resharpen the knife and cool the block on ice water (correct)
  • Retrim the block
  • Readjust and reorient the G block
  • Coat the horizontal edges of the block with wax of lower melting point

    • Why might the knife edge need to be cleaned?

  • The knife is too blunt
  • The microtome set screw is loose
  • The knife edge is coated with paraffin (correct)
  • The paraffin block is too warm

    • What can be done to prevent the formation of compressed, wrinkled, or jammed sections?

    <p>Reduce the tilt of the knife</p> Signup and view all the answers

    Why might the paraffin block need to be cooled?

    <p>The block is too warm</p> Signup and view all the answers

    What is the reason for sections expanding or disintegrating on the water bath?

    <p>Poor impregnation of tissue</p> Signup and view all the answers

    What is the remedy for a dull knife blade?

    <p>Hone and strop the knife</p> Signup and view all the answers

    Why do sections roll into a coil instead of remaining flat on the knife edge?

    <p>Blade is dull</p> Signup and view all the answers

    What can cause sections to roll up on cutting and adhere to the knife edge?

    <p>Blade is blunt</p> Signup and view all the answers

    Why do sections become grooved, scored, smeared, and deformed?

    <p>Blade is dull</p> Signup and view all the answers

    What can cause the ribbon to be curved, crooked, or uneven instead of straight?

    <p>Blunt knife edge</p> Signup and view all the answers

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