Podcast
Play an AI-generated podcast conversation about this lesson
Questions and Answers
What is a possible reason for the failure to form ribbons in G sections?
What is a possible reason for the failure to form ribbons in G sections?
- The microtome set screw is loose
- The surfaces and edges of the block are not parallel (correct)
- The paraffin wax is too soft
- The knife is blunt or dull
What can be done to fix compressed, wrinkled, or jammed sections?
What can be done to fix compressed, wrinkled, or jammed sections?
- Resharpen the knife and cool the block on ice water (correct)
- Retrim the block
- Readjust and reorient the G block
- Coat the horizontal edges of the block with wax of lower melting point
Why might the knife edge need to be cleaned?
Why might the knife edge need to be cleaned?
- The knife is too blunt
- The microtome set screw is loose
- The knife edge is coated with paraffin (correct)
- The paraffin block is too warm
What can be done to prevent the formation of compressed, wrinkled, or jammed sections?
What can be done to prevent the formation of compressed, wrinkled, or jammed sections?
Signup and view all the answers
Why might the paraffin block need to be cooled?
Why might the paraffin block need to be cooled?
Signup and view all the answers
What is the reason for sections expanding or disintegrating on the water bath?
What is the reason for sections expanding or disintegrating on the water bath?
Signup and view all the answers
What is the remedy for a dull knife blade?
What is the remedy for a dull knife blade?
Signup and view all the answers
Why do sections roll into a coil instead of remaining flat on the knife edge?
Why do sections roll into a coil instead of remaining flat on the knife edge?
Signup and view all the answers
What can cause sections to roll up on cutting and adhere to the knife edge?
What can cause sections to roll up on cutting and adhere to the knife edge?
Signup and view all the answers
Why do sections become grooved, scored, smeared, and deformed?
Why do sections become grooved, scored, smeared, and deformed?
Signup and view all the answers
What can cause the ribbon to be curved, crooked, or uneven instead of straight?
What can cause the ribbon to be curved, crooked, or uneven instead of straight?
Signup and view all the answers
