120 Questions
What type of fibers can be demonstrated using Aniline?
Delicate fibers
What is a consequence of using unbuffered formalin?
All of the above
What process is used to remove a 'burr'?
Stroping
What is involved in the freeze-substitution process?
Quenching and fixation with osmium tetroxide
What is the purpose of embedding a specimen in a medium?
To ensure stability before microtomy
What type of fibers can be visualized using Gomori silver stain?
Reticulum fibers
What is true about Paraffin?
It caves in at the top of the mold
What is the function of honing?
To remove a 'burr'
What is the purpose of fixation?
To preserve the specimen
What is used to demonstrate 'finest' fibers?
Orcein
What is the purpose of adding Cadmium or Cobalt salt to Formalin?
To prevent fat from dissolving
What is the type of incision used in autopsy for children and infants?
Straight incision
Which of the following is NOT a function performed by a Medtech?
Microscopic examination of tissues
What is the most common decalcifying agent used in histology?
Acid solution
What is the recommended stain for Nissl granules?
Toluidine blue
What is the type of honing that uses an electrical motor?
Machine honing
What is the temperature at which paraffin sections are floated in a water bath?
45-50 degrees Celsius
What is the purpose of a mordant in staining?
To increase the affinity of the dye
What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?
DMP
What is the ability of cells to respond to stimuli?
Irritability
What is the term for the replacement of one adult cell type by another?
Metaplasia
What is the fixative commonly used for embryo and bone tissue?
Bouin's
What is the type of tissue that Wharton's jelly is?
Connective tissue
What is the term for the swelling of tumor cells converted into jelly-like masses?
Mucoid degeneration
What is the color of the cell after Gram-negative staining?
Red
What is the license that needs to be renewed every 1 year?
Clinical Lab license
What is the term for the structural change brought about by disease?
Lesion
What is the class of hazardous material that includes explosives?
Explosive
What is the term for the process of programmed cell death?
Apoptosis
What is the type of staining used to demonstrate fibrin?
PAS stain
What is the penalty if the blood bank didn't throw away expired blood within 24 hours?
10 years imprisonment
What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?
Interlab quality assurance program
Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?
0.025
What type of blood banks were phased out due to the National Blood Services Act of 1994?
Commercial blood banks
What type of hospital compatibility testing is based on categorization?
Hospital based category A
Who is authorized to recruit, screen donor, and collect blood?
Either
What is the process of hormonal evaluation?
Vaginal smear
What type of microtome is the simplest?
Autotechnicon
What is the term for the study of denotation and connotation of words and messages?
Semantics
What is the most significant action to take to decrease the chance of getting sued?
Customer relationship
What type of fibers can be demonstrated using Janus Green?
Fine and delicate fibers
What is the consequence of using unbuffered formalin in tissue fixation?
Formation of brown pigment granules in blood-containing organs
What is the purpose of stroping in removing a 'burr'?
To remove the 'burr' from the blade
What is involved in the freeze-substitution process?
Quenching and filtration with osmium tetroxide
What is the purpose of embedding a specimen in a medium?
To ensure stability before placing in a block in the microtome
What type of fibers can be visualized using Gomori silver stain?
Reticulum fibers
What is true about Paraffin?
It does not solidify uniformly and generally caves in at the top of the mold
What is the purpose of using Orcein stain?
To demonstrate fine and delicate fibers
What is the consequence of using Cedarwood oil and chloroform?
It can remove Paraffin easily
What is the purpose of honing and stroping?
To sharpen and remove the 'burr' from a blade
What is the consequence of failing to dispose of contaminated blood products in a blood bank beyond 48 hours?
Imprisoned for 10 yrs
What is the purpose of adding Cadmium or Cobalt salt to Formalin?
To remove lipids
What type of incision is used in autopsy for children and infants?
Straight incision
What is the purpose of a mordant in staining?
To increase the staining power
What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?
DMP
What is the ability of cells to respond to stimuli?
Irritability
What is the term for the structural change brought about by disease?
Lesion
What is the type of tissue that Wharton's jelly is?
Connective
What is the recommended stain for Nissl granules?
Toluidine blue
What is the purpose of deparaffinization?
To remove paraffin from the tissue
What is the most common decalcifying agent used in histology?
Acid solution
What is the term for the replacement of one adult cell type by another?
Metaplasia
What is the type of fixative commonly used for embryo and bone tissue?
Bouin's
What is the term for the swelling of tumor cells converted into jelly-like masses?
Mucoid degeneration
What is the purpose of adding a mordant in staining?
To fix the stain to the tissue
What is the class of hazardous material that includes explosives?
Explosive
What is the term for the process of programmed cell death?
Apoptosis
What is the type of staining used to demonstrate fibrin?
PAS stain
What is the term for the process of programmed cell death?
Apoptosis
What is the term for the study of denotation and connotation of words and messages?
Semiotics
What is the purpose of adding Cadmium or Cobalt salt to Formalin?
To decalcify bone tissue
What type of reagent dehydrates when reacted with water and forms methanol and acetone?
Glacial acetic acid
What is the penalty if the blood bank didn't throw away expired blood within 24 hours?
License revocation
Which of the following is NOT a function performed by a Medtech?
Resolve family issues
What is the term for the structural change brought about by disease?
Lesion
What is the type of communication that studies about denotation and connotation of words and messages?
Semantics
What is the penalty if the blood bank didn't throw away expired blood within 24 hours?
10 years imprisonment
What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?
Interlab quality assurance program
Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?
0.025
What is the simplest type of microtome?
Rocking microtome
What is the primary purpose of using Orcein stain?
To demonstrate 'finest' fibers
What is the consequence of using Cedarwood oil and chloroform?
Easy removal of paraffin from tissue sections
What is the purpose of quenching in freeze-substitution?
To dehydrate tissue sections
What is the primary purpose of embedding a specimen in a medium?
To stabilize the specimen before sectioning
What type of fibers can be visualized using Gomori silver stain?
Reticulum fibers
What is the consequence of using unbuffered formalin?
All of the above
What is the primary purpose of stroping?
To remove the 'burr' from the blade
What is the primary purpose of infiltration in histology?
To infiltrate the tissue section with resin
What is the primary purpose of fixation in histology?
To fix tissue sections with formalin
What is the primary purpose of honing in histology?
To remove the 'burr' from the blade
What is the term for the structural change brought about by disease?
Lesion
What type of tissue is Wharton's jelly?
Connective
What is the ability of cells to respond to stimuli?
Irritability
What is the purpose of adding Cadmium or Cobalt salt to Formalin?
To prevent fat formation
What is the type of microtome used for rapid diagnosis?
Cryostat
What is the most common decalcifying agent used in histology?
Acid solution
What is the recommended stain for Nissl granules?
Toluidine blue
What is the purpose of a mordant in staining?
To increase the affinity of a dye for a tissue component
What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?
DMP
What is the term for the replacement of one adult cell type by another?
Metaplasia
What is the term for the state of collapse when there is insufficient oxygen supply to tissues?
Shock
What is the fixative commonly used for embryo and bone tissue?
Bouin's
What is the type of tumor that is benign and affects striated muscle?
Rhabdomyoma
What is the term for the swelling of tumor cells converted into jelly-like masses?
Mucoid degeneration
What is the temperature at which paraffin sections are floated in a water bath?
45-50°C
What is the license that needs to be renewed every 1 year?
Clinical laboratory license
What is the class of hazardous material that includes explosives?
Explosive
What is the term for programmed cell death?
Apoptosis
What is the type of staining used to demonstrate fibrin?
PAS stain
What is the term for the process of hormonal evaluation?
Assay
What is the penalty if the blood bank did not throw away expired blood within 24 hours?
10 years imprisonment
What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?
Interlab quality assurance program
Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?
0.025
What type of blood banks were phased out due to the National Blood Services Act of 1994?
Commercial blood banks
What is the process of hormonal evaluation?
Vaginal smear
What is the term for the study of denotation and connotation of words and messages?
Semantics
What is the most significant action to take to decrease the chance of getting sued?
Establish a customer relationship
What is the simplest type of microtome?
Autotechnicon
What is the type of hospital compatibility testing based on categorization?
Hospital based category A
Who is authorized to recruit, screen donor, and collect blood?
DOH
Study Notes
Histotechnology and Microscopy
- Orcein is used to demonstrate "finest" and "delicate" fibers.
- Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
- Stroping is a process used to remove the "burr" from a knife.
- Freeze-substitution involves quenching and replacement of water with paraffin.
- Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.
Special Stains
- Reticulum is visible when stained with Gomori silver stain.
- Toluidine blue is the recommended stain for nissl granules.
- Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.
Autopsy and Histopathology
- Straight incision is used in autopsy procedures for children and infants.
- Canada balsam is a permanent mounting media.
- Simple columnar is not matched correctly with the urethra.
- Microscopic examination of tissues is not performed by a Medtech.
Laboratory Techniques
- Freeze-drying techniques involve quenching and desiccation.
- Cadmium or cobalt salt is added to prevent fat in formalin.
- Anhydrous copper sulfate is used to complete dehydration.
- Acetone is used to fix tissues for rabies diagnosis.
Cellular Biology
- Irritability is the ability of cells to respond to stimuli.
- Parabasal cells have a fried egg appearance like a sunny side up.
- Rhabdomyoma is a benign tumor of striated muscle.
- Wharton's jelly is a type of connective tissue.
- Lesion refers to a structural change brought about by disease.
Microbiology
- Gram-negative cells appear red after gram staining.
- Shock occurs when there is insufficient oxygen supply to tissues.
- Apoptosis is a type of programmed cell death.
Miscellaneous
- The correct order of signing autopsy is spouse, child, father, and mother.
- Paraffin wax is the best impregnating and embedding media.
- Atresia is a congenital anomaly where there is a closure of the external auditory canal.
- Apoptosis is a type of programmed cell death.
- Toxic dehydrating agents are used in red blood cells.
- Hypertrophy is a type of cellular adaptive change.
- Supravital staining is used to stain living cells immediately after removal from the living body.
- PAS stain is used to demonstrate fibrin.
Laboratory Safety and Quality Control
- EQAP is an interlab quality assurance program.
- Categorical based hospital compatibility testing is hospital-based category A.
- HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
- RA 9165 is the Dangerous Drugs Act of 2002.
- The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
- Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
- PAMET is an acronym for the hymn "In fields of advancement and learning".
Research and Statistics
- Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
- Semantics is the study of the denotation and connotation of words and messages.
- Customer relationship is the most significant action to take to decrease the chance of getting sued.
- A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
- Endogenous refers to the dependent variable.
- Mixed research involves emerging of questions and procedures, collecting in participants' settings.
Histotechnology and Microscopy
- Orcein is used to demonstrate "finest" and "delicate" fibers.
- Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
- Stroping is a process used to remove the "burr" from a knife.
- Freeze-substitution involves quenching and replacement of water with paraffin.
- Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.
Special Stains
- Reticulum is visible when stained with Gomori silver stain.
- Toluidine blue is the recommended stain for nissl granules.
- Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.
Autopsy and Histopathology
- Straight incision is used in autopsy procedures for children and infants.
- Canada balsam is a permanent mounting media.
- Simple columnar is not matched correctly with the urethra.
- Microscopic examination of tissues is not performed by a Medtech.
Laboratory Techniques
- Freeze-drying techniques involve quenching and desiccation.
- Cadmium or cobalt salt is added to prevent fat in formalin.
- Anhydrous copper sulfate is used to complete dehydration.
- Acetone is used to fix tissues for rabies diagnosis.
Cellular Biology
- Irritability is the ability of cells to respond to stimuli.
- Parabasal cells have a fried egg appearance like a sunny side up.
- Rhabdomyoma is a benign tumor of striated muscle.
- Wharton's jelly is a type of connective tissue.
- Lesion refers to a structural change brought about by disease.
Microbiology
- Gram-negative cells appear red after gram staining.
- Shock occurs when there is insufficient oxygen supply to tissues.
- Apoptosis is a type of programmed cell death.
Miscellaneous
- The correct order of signing autopsy is spouse, child, father, and mother.
- Paraffin wax is the best impregnating and embedding media.
- Atresia is a congenital anomaly where there is a closure of the external auditory canal.
- Apoptosis is a type of programmed cell death.
- Toxic dehydrating agents are used in red blood cells.
- Hypertrophy is a type of cellular adaptive change.
- Supravital staining is used to stain living cells immediately after removal from the living body.
- PAS stain is used to demonstrate fibrin.
Laboratory Safety and Quality Control
- EQAP is an interlab quality assurance program.
- Categorical based hospital compatibility testing is hospital-based category A.
- HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
- RA 9165 is the Dangerous Drugs Act of 2002.
- The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
- Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
- PAMET is an acronym for the hymn "In fields of advancement and learning".
Research and Statistics
- Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
- Semantics is the study of the denotation and connotation of words and messages.
- Customer relationship is the most significant action to take to decrease the chance of getting sued.
- A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
- Endogenous refers to the dependent variable.
- Mixed research involves emerging of questions and procedures, collecting in participants' settings.
Histotechnology and Microscopy
- Orcein is used to demonstrate "finest" and "delicate" fibers.
- Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
- Stroping is a process used to remove the "burr" from a knife.
- Freeze-substitution involves quenching and replacement of water with paraffin.
- Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.
Special Stains
- Reticulum is visible when stained with Gomori silver stain.
- Toluidine blue is the recommended stain for nissl granules.
- Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.
Autopsy and Histopathology
- Straight incision is used in autopsy procedures for children and infants.
- Canada balsam is a permanent mounting media.
- Simple columnar is not matched correctly with the urethra.
- Microscopic examination of tissues is not performed by a Medtech.
Laboratory Techniques
- Freeze-drying techniques involve quenching and desiccation.
- Cadmium or cobalt salt is added to prevent fat in formalin.
- Anhydrous copper sulfate is used to complete dehydration.
- Acetone is used to fix tissues for rabies diagnosis.
Cellular Biology
- Irritability is the ability of cells to respond to stimuli.
- Parabasal cells have a fried egg appearance like a sunny side up.
- Rhabdomyoma is a benign tumor of striated muscle.
- Wharton's jelly is a type of connective tissue.
- Lesion refers to a structural change brought about by disease.
Microbiology
- Gram-negative cells appear red after gram staining.
- Shock occurs when there is insufficient oxygen supply to tissues.
- Apoptosis is a type of programmed cell death.
Miscellaneous
- The correct order of signing autopsy is spouse, child, father, and mother.
- Paraffin wax is the best impregnating and embedding media.
- Atresia is a congenital anomaly where there is a closure of the external auditory canal.
- Apoptosis is a type of programmed cell death.
- Toxic dehydrating agents are used in red blood cells.
- Hypertrophy is a type of cellular adaptive change.
- Supravital staining is used to stain living cells immediately after removal from the living body.
- PAS stain is used to demonstrate fibrin.
Laboratory Safety and Quality Control
- EQAP is an interlab quality assurance program.
- Categorical based hospital compatibility testing is hospital-based category A.
- HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
- RA 9165 is the Dangerous Drugs Act of 2002.
- The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
- Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
- PAMET is an acronym for the hymn "In fields of advancement and learning".
Research and Statistics
- Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
- Semantics is the study of the denotation and connotation of words and messages.
- Customer relationship is the most significant action to take to decrease the chance of getting sued.
- A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
- Endogenous refers to the dependent variable.
- Mixed research involves emerging of questions and procedures, collecting in participants' settings.
This quiz assesses knowledge of various histology and histopathology techniques, including fiber demonstration, specimen preparation, and staining methods.
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