quiz image

Histology and Histopathology Techniques

PeeN00t avatar
PeeN00t
·
·
Download

Start Quiz

Study Flashcards

120 Questions

What type of fibers can be demonstrated using Aniline?

Delicate fibers

What is a consequence of using unbuffered formalin?

All of the above

What process is used to remove a 'burr'?

Stroping

What is involved in the freeze-substitution process?

Quenching and fixation with osmium tetroxide

What is the purpose of embedding a specimen in a medium?

To ensure stability before microtomy

What type of fibers can be visualized using Gomori silver stain?

Reticulum fibers

What is true about Paraffin?

It caves in at the top of the mold

What is the function of honing?

To remove a 'burr'

What is the purpose of fixation?

To preserve the specimen

What is used to demonstrate 'finest' fibers?

Orcein

What is the purpose of adding Cadmium or Cobalt salt to Formalin?

To prevent fat from dissolving

What is the type of incision used in autopsy for children and infants?

Straight incision

Which of the following is NOT a function performed by a Medtech?

Microscopic examination of tissues

What is the most common decalcifying agent used in histology?

Acid solution

What is the recommended stain for Nissl granules?

Toluidine blue

What is the type of honing that uses an electrical motor?

Machine honing

What is the temperature at which paraffin sections are floated in a water bath?

45-50 degrees Celsius

What is the purpose of a mordant in staining?

To increase the affinity of the dye

What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?

DMP

What is the ability of cells to respond to stimuli?

Irritability

What is the term for the replacement of one adult cell type by another?

Metaplasia

What is the fixative commonly used for embryo and bone tissue?

Bouin's

What is the type of tissue that Wharton's jelly is?

Connective tissue

What is the term for the swelling of tumor cells converted into jelly-like masses?

Mucoid degeneration

What is the color of the cell after Gram-negative staining?

Red

What is the license that needs to be renewed every 1 year?

Clinical Lab license

What is the term for the structural change brought about by disease?

Lesion

What is the class of hazardous material that includes explosives?

Explosive

What is the term for the process of programmed cell death?

Apoptosis

What is the type of staining used to demonstrate fibrin?

PAS stain

What is the penalty if the blood bank didn't throw away expired blood within 24 hours?

10 years imprisonment

What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?

Interlab quality assurance program

Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?

0.025

What type of blood banks were phased out due to the National Blood Services Act of 1994?

Commercial blood banks

What type of hospital compatibility testing is based on categorization?

Hospital based category A

Who is authorized to recruit, screen donor, and collect blood?

Either

What is the process of hormonal evaluation?

Vaginal smear

What type of microtome is the simplest?

Autotechnicon

What is the term for the study of denotation and connotation of words and messages?

Semantics

What is the most significant action to take to decrease the chance of getting sued?

Customer relationship

What type of fibers can be demonstrated using Janus Green?

Fine and delicate fibers

What is the consequence of using unbuffered formalin in tissue fixation?

Formation of brown pigment granules in blood-containing organs

What is the purpose of stroping in removing a 'burr'?

To remove the 'burr' from the blade

What is involved in the freeze-substitution process?

Quenching and filtration with osmium tetroxide

What is the purpose of embedding a specimen in a medium?

To ensure stability before placing in a block in the microtome

What type of fibers can be visualized using Gomori silver stain?

Reticulum fibers

What is true about Paraffin?

It does not solidify uniformly and generally caves in at the top of the mold

What is the purpose of using Orcein stain?

To demonstrate fine and delicate fibers

What is the consequence of using Cedarwood oil and chloroform?

It can remove Paraffin easily

What is the purpose of honing and stroping?

To sharpen and remove the 'burr' from a blade

What is the consequence of failing to dispose of contaminated blood products in a blood bank beyond 48 hours?

Imprisoned for 10 yrs

What is the purpose of adding Cadmium or Cobalt salt to Formalin?

To remove lipids

What type of incision is used in autopsy for children and infants?

Straight incision

What is the purpose of a mordant in staining?

To increase the staining power

What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?

DMP

What is the ability of cells to respond to stimuli?

Irritability

What is the term for the structural change brought about by disease?

Lesion

What is the type of tissue that Wharton's jelly is?

Connective

What is the recommended stain for Nissl granules?

Toluidine blue

What is the purpose of deparaffinization?

To remove paraffin from the tissue

What is the most common decalcifying agent used in histology?

Acid solution

What is the term for the replacement of one adult cell type by another?

Metaplasia

What is the type of fixative commonly used for embryo and bone tissue?

Bouin's

What is the term for the swelling of tumor cells converted into jelly-like masses?

Mucoid degeneration

What is the purpose of adding a mordant in staining?

To fix the stain to the tissue

What is the class of hazardous material that includes explosives?

Explosive

What is the term for the process of programmed cell death?

Apoptosis

What is the type of staining used to demonstrate fibrin?

PAS stain

What is the term for the process of programmed cell death?

Apoptosis

What is the term for the study of denotation and connotation of words and messages?

Semiotics

What is the purpose of adding Cadmium or Cobalt salt to Formalin?

To decalcify bone tissue

What type of reagent dehydrates when reacted with water and forms methanol and acetone?

Glacial acetic acid

What is the penalty if the blood bank didn't throw away expired blood within 24 hours?

License revocation

Which of the following is NOT a function performed by a Medtech?

Resolve family issues

What is the term for the structural change brought about by disease?

Lesion

What is the type of communication that studies about denotation and connotation of words and messages?

Semantics

What is the penalty if the blood bank didn't throw away expired blood within 24 hours?

10 years imprisonment

What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?

Interlab quality assurance program

Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?

0.025

What is the simplest type of microtome?

Rocking microtome

What is the primary purpose of using Orcein stain?

To demonstrate 'finest' fibers

What is the consequence of using Cedarwood oil and chloroform?

Easy removal of paraffin from tissue sections

What is the purpose of quenching in freeze-substitution?

To dehydrate tissue sections

What is the primary purpose of embedding a specimen in a medium?

To stabilize the specimen before sectioning

What type of fibers can be visualized using Gomori silver stain?

Reticulum fibers

What is the consequence of using unbuffered formalin?

All of the above

What is the primary purpose of stroping?

To remove the 'burr' from the blade

What is the primary purpose of infiltration in histology?

To infiltrate the tissue section with resin

What is the primary purpose of fixation in histology?

To fix tissue sections with formalin

What is the primary purpose of honing in histology?

To remove the 'burr' from the blade

What is the term for the structural change brought about by disease?

Lesion

What type of tissue is Wharton's jelly?

Connective

What is the ability of cells to respond to stimuli?

Irritability

What is the purpose of adding Cadmium or Cobalt salt to Formalin?

To prevent fat formation

What is the type of microtome used for rapid diagnosis?

Cryostat

What is the most common decalcifying agent used in histology?

Acid solution

What is the recommended stain for Nissl granules?

Toluidine blue

What is the purpose of a mordant in staining?

To increase the affinity of a dye for a tissue component

What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?

DMP

What is the term for the replacement of one adult cell type by another?

Metaplasia

What is the term for the state of collapse when there is insufficient oxygen supply to tissues?

Shock

What is the fixative commonly used for embryo and bone tissue?

Bouin's

What is the type of tumor that is benign and affects striated muscle?

Rhabdomyoma

What is the term for the swelling of tumor cells converted into jelly-like masses?

Mucoid degeneration

What is the temperature at which paraffin sections are floated in a water bath?

45-50°C

What is the license that needs to be renewed every 1 year?

Clinical laboratory license

What is the class of hazardous material that includes explosives?

Explosive

What is the term for programmed cell death?

Apoptosis

What is the type of staining used to demonstrate fibrin?

PAS stain

What is the term for the process of hormonal evaluation?

Assay

What is the penalty if the blood bank did not throw away expired blood within 24 hours?

10 years imprisonment

What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?

Interlab quality assurance program

Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?

0.025

What type of blood banks were phased out due to the National Blood Services Act of 1994?

Commercial blood banks

What is the process of hormonal evaluation?

Vaginal smear

What is the term for the study of denotation and connotation of words and messages?

Semantics

What is the most significant action to take to decrease the chance of getting sued?

Establish a customer relationship

What is the simplest type of microtome?

Autotechnicon

What is the type of hospital compatibility testing based on categorization?

Hospital based category A

Who is authorized to recruit, screen donor, and collect blood?

DOH

Study Notes

Histotechnology and Microscopy

  • Orcein is used to demonstrate "finest" and "delicate" fibers.
  • Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
  • Stroping is a process used to remove the "burr" from a knife.
  • Freeze-substitution involves quenching and replacement of water with paraffin.
  • Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.

Special Stains

  • Reticulum is visible when stained with Gomori silver stain.
  • Toluidine blue is the recommended stain for nissl granules.
  • Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.

Autopsy and Histopathology

  • Straight incision is used in autopsy procedures for children and infants.
  • Canada balsam is a permanent mounting media.
  • Simple columnar is not matched correctly with the urethra.
  • Microscopic examination of tissues is not performed by a Medtech.

Laboratory Techniques

  • Freeze-drying techniques involve quenching and desiccation.
  • Cadmium or cobalt salt is added to prevent fat in formalin.
  • Anhydrous copper sulfate is used to complete dehydration.
  • Acetone is used to fix tissues for rabies diagnosis.

Cellular Biology

  • Irritability is the ability of cells to respond to stimuli.
  • Parabasal cells have a fried egg appearance like a sunny side up.
  • Rhabdomyoma is a benign tumor of striated muscle.
  • Wharton's jelly is a type of connective tissue.
  • Lesion refers to a structural change brought about by disease.

Microbiology

  • Gram-negative cells appear red after gram staining.
  • Shock occurs when there is insufficient oxygen supply to tissues.
  • Apoptosis is a type of programmed cell death.

Miscellaneous

  • The correct order of signing autopsy is spouse, child, father, and mother.
  • Paraffin wax is the best impregnating and embedding media.
  • Atresia is a congenital anomaly where there is a closure of the external auditory canal.
  • Apoptosis is a type of programmed cell death.
  • Toxic dehydrating agents are used in red blood cells.
  • Hypertrophy is a type of cellular adaptive change.
  • Supravital staining is used to stain living cells immediately after removal from the living body.
  • PAS stain is used to demonstrate fibrin.

Laboratory Safety and Quality Control

  • EQAP is an interlab quality assurance program.
  • Categorical based hospital compatibility testing is hospital-based category A.
  • HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
  • RA 9165 is the Dangerous Drugs Act of 2002.
  • The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
  • Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
  • PAMET is an acronym for the hymn "In fields of advancement and learning".

Research and Statistics

  • Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
  • Semantics is the study of the denotation and connotation of words and messages.
  • Customer relationship is the most significant action to take to decrease the chance of getting sued.
  • A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
  • Endogenous refers to the dependent variable.
  • Mixed research involves emerging of questions and procedures, collecting in participants' settings.

Histotechnology and Microscopy

  • Orcein is used to demonstrate "finest" and "delicate" fibers.
  • Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
  • Stroping is a process used to remove the "burr" from a knife.
  • Freeze-substitution involves quenching and replacement of water with paraffin.
  • Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.

Special Stains

  • Reticulum is visible when stained with Gomori silver stain.
  • Toluidine blue is the recommended stain for nissl granules.
  • Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.

Autopsy and Histopathology

  • Straight incision is used in autopsy procedures for children and infants.
  • Canada balsam is a permanent mounting media.
  • Simple columnar is not matched correctly with the urethra.
  • Microscopic examination of tissues is not performed by a Medtech.

Laboratory Techniques

  • Freeze-drying techniques involve quenching and desiccation.
  • Cadmium or cobalt salt is added to prevent fat in formalin.
  • Anhydrous copper sulfate is used to complete dehydration.
  • Acetone is used to fix tissues for rabies diagnosis.

Cellular Biology

  • Irritability is the ability of cells to respond to stimuli.
  • Parabasal cells have a fried egg appearance like a sunny side up.
  • Rhabdomyoma is a benign tumor of striated muscle.
  • Wharton's jelly is a type of connective tissue.
  • Lesion refers to a structural change brought about by disease.

Microbiology

  • Gram-negative cells appear red after gram staining.
  • Shock occurs when there is insufficient oxygen supply to tissues.
  • Apoptosis is a type of programmed cell death.

Miscellaneous

  • The correct order of signing autopsy is spouse, child, father, and mother.
  • Paraffin wax is the best impregnating and embedding media.
  • Atresia is a congenital anomaly where there is a closure of the external auditory canal.
  • Apoptosis is a type of programmed cell death.
  • Toxic dehydrating agents are used in red blood cells.
  • Hypertrophy is a type of cellular adaptive change.
  • Supravital staining is used to stain living cells immediately after removal from the living body.
  • PAS stain is used to demonstrate fibrin.

Laboratory Safety and Quality Control

  • EQAP is an interlab quality assurance program.
  • Categorical based hospital compatibility testing is hospital-based category A.
  • HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
  • RA 9165 is the Dangerous Drugs Act of 2002.
  • The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
  • Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
  • PAMET is an acronym for the hymn "In fields of advancement and learning".

Research and Statistics

  • Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
  • Semantics is the study of the denotation and connotation of words and messages.
  • Customer relationship is the most significant action to take to decrease the chance of getting sued.
  • A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
  • Endogenous refers to the dependent variable.
  • Mixed research involves emerging of questions and procedures, collecting in participants' settings.

Histotechnology and Microscopy

  • Orcein is used to demonstrate "finest" and "delicate" fibers.
  • Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
  • Stroping is a process used to remove the "burr" from a knife.
  • Freeze-substitution involves quenching and replacement of water with paraffin.
  • Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.

Special Stains

  • Reticulum is visible when stained with Gomori silver stain.
  • Toluidine blue is the recommended stain for nissl granules.
  • Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.

Autopsy and Histopathology

  • Straight incision is used in autopsy procedures for children and infants.
  • Canada balsam is a permanent mounting media.
  • Simple columnar is not matched correctly with the urethra.
  • Microscopic examination of tissues is not performed by a Medtech.

Laboratory Techniques

  • Freeze-drying techniques involve quenching and desiccation.
  • Cadmium or cobalt salt is added to prevent fat in formalin.
  • Anhydrous copper sulfate is used to complete dehydration.
  • Acetone is used to fix tissues for rabies diagnosis.

Cellular Biology

  • Irritability is the ability of cells to respond to stimuli.
  • Parabasal cells have a fried egg appearance like a sunny side up.
  • Rhabdomyoma is a benign tumor of striated muscle.
  • Wharton's jelly is a type of connective tissue.
  • Lesion refers to a structural change brought about by disease.

Microbiology

  • Gram-negative cells appear red after gram staining.
  • Shock occurs when there is insufficient oxygen supply to tissues.
  • Apoptosis is a type of programmed cell death.

Miscellaneous

  • The correct order of signing autopsy is spouse, child, father, and mother.
  • Paraffin wax is the best impregnating and embedding media.
  • Atresia is a congenital anomaly where there is a closure of the external auditory canal.
  • Apoptosis is a type of programmed cell death.
  • Toxic dehydrating agents are used in red blood cells.
  • Hypertrophy is a type of cellular adaptive change.
  • Supravital staining is used to stain living cells immediately after removal from the living body.
  • PAS stain is used to demonstrate fibrin.

Laboratory Safety and Quality Control

  • EQAP is an interlab quality assurance program.
  • Categorical based hospital compatibility testing is hospital-based category A.
  • HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
  • RA 9165 is the Dangerous Drugs Act of 2002.
  • The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
  • Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
  • PAMET is an acronym for the hymn "In fields of advancement and learning".

Research and Statistics

  • Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
  • Semantics is the study of the denotation and connotation of words and messages.
  • Customer relationship is the most significant action to take to decrease the chance of getting sued.
  • A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
  • Endogenous refers to the dependent variable.
  • Mixed research involves emerging of questions and procedures, collecting in participants' settings.

This quiz assesses knowledge of various histology and histopathology techniques, including fiber demonstration, specimen preparation, and staining methods.

Make Your Own Quizzes and Flashcards

Convert your notes into interactive study material.

Get started for free

More Quizzes Like This

Use Quizgecko on...
Browser
Browser