Histology and Histopathology Techniques
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Questions and Answers

What type of fibers can be demonstrated using Aniline?

  • Nerve fibers
  • Coarse fibers
  • Muscle fibers
  • Delicate fibers (correct)

    • What is a consequence of using unbuffered formalin?

  • Reduced quality of fixation
  • Formation of formic acid
  • Formation of brown pigment granules
  • All of the above (correct)

    • What process is used to remove a 'burr'?

  • Stroping (correct)
  • Polishing
  • Grinding
  • Honing

    • What is involved in the freeze-substitution process?

    <p>Quenching and fixation with osmium tetroxide</p> Signup and view all the answers

    What is the purpose of embedding a specimen in a medium?

    <p>To ensure stability before microtomy</p> Signup and view all the answers

    What type of fibers can be visualized using Gomori silver stain?

    <p>Reticulum fibers</p> Signup and view all the answers

    What is true about Paraffin?

    <p>It caves in at the top of the mold</p> Signup and view all the answers

    What is the function of honing?

    <p>To remove a 'burr'</p> Signup and view all the answers

    What is the purpose of fixation?

    <p>To preserve the specimen</p> Signup and view all the answers

    What is used to demonstrate 'finest' fibers?

    <p>Orcein</p> Signup and view all the answers

    What is the purpose of adding Cadmium or Cobalt salt to Formalin?

    <p>To prevent fat from dissolving</p> Signup and view all the answers

    What is the type of incision used in autopsy for children and infants?

    <p>Straight incision</p> Signup and view all the answers

    Which of the following is NOT a function performed by a Medtech?

    <p>Microscopic examination of tissues</p> Signup and view all the answers

    What is the most common decalcifying agent used in histology?

    <p>Acid solution</p> Signup and view all the answers

    What is the recommended stain for Nissl granules?

    <p>Toluidine blue</p> Signup and view all the answers

    What is the type of honing that uses an electrical motor?

    <p>Machine honing</p> Signup and view all the answers

    What is the temperature at which paraffin sections are floated in a water bath?

    <p>45-50 degrees Celsius</p> Signup and view all the answers

    What is the purpose of a mordant in staining?

    <p>To increase the affinity of the dye</p> Signup and view all the answers

    What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?

    <p>DMP</p> Signup and view all the answers

    What is the ability of cells to respond to stimuli?

    <p>Irritability</p> Signup and view all the answers

    What is the term for the replacement of one adult cell type by another?

    <p>Metaplasia</p> Signup and view all the answers

    What is the fixative commonly used for embryo and bone tissue?

    <p>Bouin's</p> Signup and view all the answers

    What is the type of tissue that Wharton's jelly is?

    <p>Connective tissue</p> Signup and view all the answers

    What is the term for the swelling of tumor cells converted into jelly-like masses?

    <p>Mucoid degeneration</p> Signup and view all the answers

    What is the color of the cell after Gram-negative staining?

    <p>Red</p> Signup and view all the answers

    What is the license that needs to be renewed every 1 year?

    <p>Clinical Lab license</p> Signup and view all the answers

    What is the term for the structural change brought about by disease?

    <p>Lesion</p> Signup and view all the answers

    What is the class of hazardous material that includes explosives?

    <p>Explosive</p> Signup and view all the answers

    What is the term for the process of programmed cell death?

    <p>Apoptosis</p> Signup and view all the answers

    What is the type of staining used to demonstrate fibrin?

    <p>PAS stain</p> Signup and view all the answers

    What is the penalty if the blood bank didn't throw away expired blood within 24 hours?

    <p>10 years imprisonment</p> Signup and view all the answers

    What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?

    <p>Interlab quality assurance program</p> Signup and view all the answers

    Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?

    <p>0.025</p> Signup and view all the answers

    What type of blood banks were phased out due to the National Blood Services Act of 1994?

    <p>Commercial blood banks</p> Signup and view all the answers

    What type of hospital compatibility testing is based on categorization?

    <p>Hospital based category A</p> Signup and view all the answers

    Who is authorized to recruit, screen donor, and collect blood?

    <p>Either</p> Signup and view all the answers

    What is the process of hormonal evaluation?

    <p>Vaginal smear</p> Signup and view all the answers

    What type of microtome is the simplest?

    <p>Autotechnicon</p> Signup and view all the answers

    What is the term for the study of denotation and connotation of words and messages?

    <p>Semantics</p> Signup and view all the answers

    What is the most significant action to take to decrease the chance of getting sued?

    <p>Customer relationship</p> Signup and view all the answers

    What type of fibers can be demonstrated using Janus Green?

    <p>Fine and delicate fibers</p> Signup and view all the answers

    What is the consequence of using unbuffered formalin in tissue fixation?

    <p>Formation of brown pigment granules in blood-containing organs</p> Signup and view all the answers

    What is the purpose of stroping in removing a 'burr'?

    <p>To remove the 'burr' from the blade</p> Signup and view all the answers

    What is involved in the freeze-substitution process?

    <p>Quenching and filtration with osmium tetroxide</p> Signup and view all the answers

    What is the purpose of embedding a specimen in a medium?

    <p>To ensure stability before placing in a block in the microtome</p> Signup and view all the answers

    What type of fibers can be visualized using Gomori silver stain?

    <p>Reticulum fibers</p> Signup and view all the answers

    What is true about Paraffin?

    <p>It does not solidify uniformly and generally caves in at the top of the mold</p> Signup and view all the answers

    What is the purpose of using Orcein stain?

    <p>To demonstrate fine and delicate fibers</p> Signup and view all the answers

    What is the consequence of using Cedarwood oil and chloroform?

    <p>It can remove Paraffin easily</p> Signup and view all the answers

    What is the purpose of honing and stroping?

    <p>To sharpen and remove the 'burr' from a blade</p> Signup and view all the answers

    What is the consequence of failing to dispose of contaminated blood products in a blood bank beyond 48 hours?

    <p>Imprisoned for 10 yrs</p> Signup and view all the answers

    What is the purpose of adding Cadmium or Cobalt salt to Formalin?

    <p>To remove lipids</p> Signup and view all the answers

    What type of incision is used in autopsy for children and infants?

    <p>Straight incision</p> Signup and view all the answers

    What is the purpose of a mordant in staining?

    <p>To increase the staining power</p> Signup and view all the answers

    What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?

    <p>DMP</p> Signup and view all the answers

    What is the ability of cells to respond to stimuli?

    <p>Irritability</p> Signup and view all the answers

    What is the term for the structural change brought about by disease?

    <p>Lesion</p> Signup and view all the answers

    What is the type of tissue that Wharton's jelly is?

    <p>Connective</p> Signup and view all the answers

    What is the recommended stain for Nissl granules?

    <p>Toluidine blue</p> Signup and view all the answers

    What is the purpose of deparaffinization?

    <p>To remove paraffin from the tissue</p> Signup and view all the answers

    What is the most common decalcifying agent used in histology?

    <p>Acid solution</p> Signup and view all the answers

    What is the term for the replacement of one adult cell type by another?

    <p>Metaplasia</p> Signup and view all the answers

    What is the type of fixative commonly used for embryo and bone tissue?

    <p>Bouin's</p> Signup and view all the answers

    What is the term for the swelling of tumor cells converted into jelly-like masses?

    <p>Mucoid degeneration</p> Signup and view all the answers

    What is the purpose of adding a mordant in staining?

    <p>To fix the stain to the tissue</p> Signup and view all the answers

    What is the class of hazardous material that includes explosives?

    <p>Explosive</p> Signup and view all the answers

    What is the term for the process of programmed cell death?

    <p>Apoptosis</p> Signup and view all the answers

    What is the type of staining used to demonstrate fibrin?

    <p>PAS stain</p> Signup and view all the answers

    What is the term for the process of programmed cell death?

    <p>Apoptosis</p> Signup and view all the answers

    What is the term for the study of denotation and connotation of words and messages?

    <p>Semiotics</p> Signup and view all the answers

    What is the purpose of adding Cadmium or Cobalt salt to Formalin?

    <p>To decalcify bone tissue</p> Signup and view all the answers

    What type of reagent dehydrates when reacted with water and forms methanol and acetone?

    <p>Glacial acetic acid</p> Signup and view all the answers

    What is the penalty if the blood bank didn't throw away expired blood within 24 hours?

    <p>License revocation</p> Signup and view all the answers

    Which of the following is NOT a function performed by a Medtech?

    <p>Resolve family issues</p> Signup and view all the answers

    What is the term for the structural change brought about by disease?

    <p>Lesion</p> Signup and view all the answers

    What is the type of communication that studies about denotation and connotation of words and messages?

    <p>Semantics</p> Signup and view all the answers

    What is the penalty if the blood bank didn't throw away expired blood within 24 hours?

    <p>10 years imprisonment</p> Signup and view all the answers

    What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?

    <p>Interlab quality assurance program</p> Signup and view all the answers

    Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?

    <p>0.025</p> Signup and view all the answers

    What is the simplest type of microtome?

    <p>Rocking microtome</p> Signup and view all the answers

    What is the primary purpose of using Orcein stain?

    <p>To demonstrate 'finest' fibers</p> Signup and view all the answers

    What is the consequence of using Cedarwood oil and chloroform?

    <p>Easy removal of paraffin from tissue sections</p> Signup and view all the answers

    What is the purpose of quenching in freeze-substitution?

    <p>To dehydrate tissue sections</p> Signup and view all the answers

    What is the primary purpose of embedding a specimen in a medium?

    <p>To stabilize the specimen before sectioning</p> Signup and view all the answers

    What type of fibers can be visualized using Gomori silver stain?

    <p>Reticulum fibers</p> Signup and view all the answers

    What is the consequence of using unbuffered formalin?

    <p>All of the above</p> Signup and view all the answers

    What is the primary purpose of stroping?

    <p>To remove the 'burr' from the blade</p> Signup and view all the answers

    What is the primary purpose of infiltration in histology?

    <p>To infiltrate the tissue section with resin</p> Signup and view all the answers

    What is the primary purpose of fixation in histology?

    <p>To fix tissue sections with formalin</p> Signup and view all the answers

    What is the primary purpose of honing in histology?

    <p>To remove the 'burr' from the blade</p> Signup and view all the answers

    What is the term for the structural change brought about by disease?

    <p>Lesion</p> Signup and view all the answers

    What type of tissue is Wharton's jelly?

    <p>Connective</p> Signup and view all the answers

    What is the ability of cells to respond to stimuli?

    <p>Irritability</p> Signup and view all the answers

    What is the purpose of adding Cadmium or Cobalt salt to Formalin?

    <p>To prevent fat formation</p> Signup and view all the answers

    What is the type of microtome used for rapid diagnosis?

    <p>Cryostat</p> Signup and view all the answers

    What is the most common decalcifying agent used in histology?

    <p>Acid solution</p> Signup and view all the answers

    What is the recommended stain for Nissl granules?

    <p>Toluidine blue</p> Signup and view all the answers

    What is the purpose of a mordant in staining?

    <p>To increase the affinity of a dye for a tissue component</p> Signup and view all the answers

    What is the type of reagent that dehydrates when reacted with water and forms methanol and acetone?

    <p>DMP</p> Signup and view all the answers

    What is the term for the replacement of one adult cell type by another?

    <p>Metaplasia</p> Signup and view all the answers

    What is the term for the state of collapse when there is insufficient oxygen supply to tissues?

    <p>Shock</p> Signup and view all the answers

    What is the fixative commonly used for embryo and bone tissue?

    <p>Bouin's</p> Signup and view all the answers

    What is the type of tumor that is benign and affects striated muscle?

    <p>Rhabdomyoma</p> Signup and view all the answers

    What is the term for the swelling of tumor cells converted into jelly-like masses?

    <p>Mucoid degeneration</p> Signup and view all the answers

    What is the temperature at which paraffin sections are floated in a water bath?

    <p>45-50°C</p> Signup and view all the answers

    What is the license that needs to be renewed every 1 year?

    <p>Clinical laboratory license</p> Signup and view all the answers

    What is the class of hazardous material that includes explosives?

    <p>Explosive</p> Signup and view all the answers

    What is the term for programmed cell death?

    <p>Apoptosis</p> Signup and view all the answers

    What is the type of staining used to demonstrate fibrin?

    <p>PAS stain</p> Signup and view all the answers

    What is the term for the process of hormonal evaluation?

    <p>Assay</p> Signup and view all the answers

    What is the penalty if the blood bank did not throw away expired blood within 24 hours?

    <p>10 years imprisonment</p> Signup and view all the answers

    What type of program is used to ensure the quality of different labs performing a test on a sample of unknown concentration?

    <p>Interlab quality assurance program</p> Signup and view all the answers

    Which of the following p-values may be interpreted as showing a statistically significant difference or association with a confidence level of 95%?

    <p>0.025</p> Signup and view all the answers

    What type of blood banks were phased out due to the National Blood Services Act of 1994?

    <p>Commercial blood banks</p> Signup and view all the answers

    What is the process of hormonal evaluation?

    <p>Vaginal smear</p> Signup and view all the answers

    What is the term for the study of denotation and connotation of words and messages?

    <p>Semantics</p> Signup and view all the answers

    What is the most significant action to take to decrease the chance of getting sued?

    <p>Establish a customer relationship</p> Signup and view all the answers

    What is the simplest type of microtome?

    <p>Autotechnicon</p> Signup and view all the answers

    What is the type of hospital compatibility testing based on categorization?

    <p>Hospital based category A</p> Signup and view all the answers

    Who is authorized to recruit, screen donor, and collect blood?

    <p>DOH</p> Signup and view all the answers

    Study Notes

    Histotechnology and Microscopy

    • Orcein is used to demonstrate "finest" and "delicate" fibers.
    • Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
    • Stroping is a process used to remove the "burr" from a knife.
    • Freeze-substitution involves quenching and replacement of water with paraffin.
    • Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.

    Special Stains

    • Reticulum is visible when stained with Gomori silver stain.
    • Toluidine blue is the recommended stain for nissl granules.
    • Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.

    Autopsy and Histopathology

    • Straight incision is used in autopsy procedures for children and infants.
    • Canada balsam is a permanent mounting media.
    • Simple columnar is not matched correctly with the urethra.
    • Microscopic examination of tissues is not performed by a Medtech.

    Laboratory Techniques

    • Freeze-drying techniques involve quenching and desiccation.
    • Cadmium or cobalt salt is added to prevent fat in formalin.
    • Anhydrous copper sulfate is used to complete dehydration.
    • Acetone is used to fix tissues for rabies diagnosis.

    Cellular Biology

    • Irritability is the ability of cells to respond to stimuli.
    • Parabasal cells have a fried egg appearance like a sunny side up.
    • Rhabdomyoma is a benign tumor of striated muscle.
    • Wharton's jelly is a type of connective tissue.
    • Lesion refers to a structural change brought about by disease.

    Microbiology

    • Gram-negative cells appear red after gram staining.
    • Shock occurs when there is insufficient oxygen supply to tissues.
    • Apoptosis is a type of programmed cell death.

    Miscellaneous

    • The correct order of signing autopsy is spouse, child, father, and mother.
    • Paraffin wax is the best impregnating and embedding media.
    • Atresia is a congenital anomaly where there is a closure of the external auditory canal.
    • Apoptosis is a type of programmed cell death.
    • Toxic dehydrating agents are used in red blood cells.
    • Hypertrophy is a type of cellular adaptive change.
    • Supravital staining is used to stain living cells immediately after removal from the living body.
    • PAS stain is used to demonstrate fibrin.

    Laboratory Safety and Quality Control

    • EQAP is an interlab quality assurance program.
    • Categorical based hospital compatibility testing is hospital-based category A.
    • HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
    • RA 9165 is the Dangerous Drugs Act of 2002.
    • The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
    • Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
    • PAMET is an acronym for the hymn "In fields of advancement and learning".

    Research and Statistics

    • Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
    • Semantics is the study of the denotation and connotation of words and messages.
    • Customer relationship is the most significant action to take to decrease the chance of getting sued.
    • A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
    • Endogenous refers to the dependent variable.
    • Mixed research involves emerging of questions and procedures, collecting in participants' settings.

    Histotechnology and Microscopy

    • Orcein is used to demonstrate "finest" and "delicate" fibers.
    • Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
    • Stroping is a process used to remove the "burr" from a knife.
    • Freeze-substitution involves quenching and replacement of water with paraffin.
    • Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.

    Special Stains

    • Reticulum is visible when stained with Gomori silver stain.
    • Toluidine blue is the recommended stain for nissl granules.
    • Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.

    Autopsy and Histopathology

    • Straight incision is used in autopsy procedures for children and infants.
    • Canada balsam is a permanent mounting media.
    • Simple columnar is not matched correctly with the urethra.
    • Microscopic examination of tissues is not performed by a Medtech.

    Laboratory Techniques

    • Freeze-drying techniques involve quenching and desiccation.
    • Cadmium or cobalt salt is added to prevent fat in formalin.
    • Anhydrous copper sulfate is used to complete dehydration.
    • Acetone is used to fix tissues for rabies diagnosis.

    Cellular Biology

    • Irritability is the ability of cells to respond to stimuli.
    • Parabasal cells have a fried egg appearance like a sunny side up.
    • Rhabdomyoma is a benign tumor of striated muscle.
    • Wharton's jelly is a type of connective tissue.
    • Lesion refers to a structural change brought about by disease.

    Microbiology

    • Gram-negative cells appear red after gram staining.
    • Shock occurs when there is insufficient oxygen supply to tissues.
    • Apoptosis is a type of programmed cell death.

    Miscellaneous

    • The correct order of signing autopsy is spouse, child, father, and mother.
    • Paraffin wax is the best impregnating and embedding media.
    • Atresia is a congenital anomaly where there is a closure of the external auditory canal.
    • Apoptosis is a type of programmed cell death.
    • Toxic dehydrating agents are used in red blood cells.
    • Hypertrophy is a type of cellular adaptive change.
    • Supravital staining is used to stain living cells immediately after removal from the living body.
    • PAS stain is used to demonstrate fibrin.

    Laboratory Safety and Quality Control

    • EQAP is an interlab quality assurance program.
    • Categorical based hospital compatibility testing is hospital-based category A.
    • HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
    • RA 9165 is the Dangerous Drugs Act of 2002.
    • The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
    • Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
    • PAMET is an acronym for the hymn "In fields of advancement and learning".

    Research and Statistics

    • Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
    • Semantics is the study of the denotation and connotation of words and messages.
    • Customer relationship is the most significant action to take to decrease the chance of getting sued.
    • A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
    • Endogenous refers to the dependent variable.
    • Mixed research involves emerging of questions and procedures, collecting in participants' settings.

    Histotechnology and Microscopy

    • Orcein is used to demonstrate "finest" and "delicate" fibers.
    • Unbuffered formalin can lead to the formation of formic acid, brown pigment granules in blood-containing organs, and reduce quality.
    • Stroping is a process used to remove the "burr" from a knife.
    • Freeze-substitution involves quenching and replacement of water with paraffin.
    • Embedding is a process in which a specimen is placed in a medium to ensure stability before being placed in a block in a microtome.

    Special Stains

    • Reticulum is visible when stained with Gomori silver stain.
    • Toluidine blue is the recommended stain for nissl granules.
    • Paraffin does not solidify uniformly, and it generally caves in at the top of the mold.

    Autopsy and Histopathology

    • Straight incision is used in autopsy procedures for children and infants.
    • Canada balsam is a permanent mounting media.
    • Simple columnar is not matched correctly with the urethra.
    • Microscopic examination of tissues is not performed by a Medtech.

    Laboratory Techniques

    • Freeze-drying techniques involve quenching and desiccation.
    • Cadmium or cobalt salt is added to prevent fat in formalin.
    • Anhydrous copper sulfate is used to complete dehydration.
    • Acetone is used to fix tissues for rabies diagnosis.

    Cellular Biology

    • Irritability is the ability of cells to respond to stimuli.
    • Parabasal cells have a fried egg appearance like a sunny side up.
    • Rhabdomyoma is a benign tumor of striated muscle.
    • Wharton's jelly is a type of connective tissue.
    • Lesion refers to a structural change brought about by disease.

    Microbiology

    • Gram-negative cells appear red after gram staining.
    • Shock occurs when there is insufficient oxygen supply to tissues.
    • Apoptosis is a type of programmed cell death.

    Miscellaneous

    • The correct order of signing autopsy is spouse, child, father, and mother.
    • Paraffin wax is the best impregnating and embedding media.
    • Atresia is a congenital anomaly where there is a closure of the external auditory canal.
    • Apoptosis is a type of programmed cell death.
    • Toxic dehydrating agents are used in red blood cells.
    • Hypertrophy is a type of cellular adaptive change.
    • Supravital staining is used to stain living cells immediately after removal from the living body.
    • PAS stain is used to demonstrate fibrin.

    Laboratory Safety and Quality Control

    • EQAP is an interlab quality assurance program.
    • Categorical based hospital compatibility testing is hospital-based category A.
    • HIV window period is 1-4 weeks, and it shows negative results when tested but can still infect.
    • RA 9165 is the Dangerous Drugs Act of 2002.
    • The penalty for not throwing away expired blood within 24 hours is 10 years imprisonment.
    • Newborn Screening Act is exempt from testing if it is against the parent's religious beliefs.
    • PAMET is an acronym for the hymn "In fields of advancement and learning".

    Research and Statistics

    • Flexible budgeting is a type of operational budgeting where the budget is based on a variable workload.
    • Semantics is the study of the denotation and connotation of words and messages.
    • Customer relationship is the most significant action to take to decrease the chance of getting sued.
    • A theoretical framework explains why the problem under investigation exists and its connection to certain factors.
    • Endogenous refers to the dependent variable.
    • Mixed research involves emerging of questions and procedures, collecting in participants' settings.

    Studying That Suits You

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    Description

    This quiz assesses knowledge of various histology and histopathology techniques, including fiber demonstration, specimen preparation, and staining methods.

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